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Iilayibrari ezenziwe kwangaphambili ze-DNBSEQ
I-DNBSEQ, eyaphuhliswa yi-MGI, yitekhnoloji entsha ye-NGS eye yakwazi ukunciphisa iindleko zokulandelelana nokunyusa umthamo. Ukulungiswa kweelayibrari ze-DNBSEQ kubandakanya ukuqhekeka kwe-DNA, ukulungiswa kwe-ssDNA, kunye nokwandiswa kwesangqa esijikelezayo ukuze kufunyanwe ii-nanoballs ze-DNA (DNB). Ezi emva koko zilayishwa kumphezulu oqinileyo kwaye emva koko zilandelelaniswe yi-combinatorial Probe-Anchor Synthesis (cPAS). Itekhnoloji ye-DNBSEQ idibanisa iingenelo zokuba nesantya esiphantsi sempazamo yokukhulisa kunye nokusebenzisa iipateni zempazamo ezinoxinano oluphezulu kunye nee-nanoballs, nto leyo ekhokelela kulandelelwano olune-throughput ephezulu kunye nokuchaneka.
Inkonzo yethu yokulandelelanisa iilayibrari eyenziwe kwangaphambili ivumela abathengi ukuba balungiselele iilayibrari zokulandelelanisa ii-Illumina ezivela kwimithombo eyahlukeneyo (i-mRNA, i-genome epheleleyo, i-amplicon, iilayibrari ezili-10x, phakathi kwezinye), eziguqulwa zibe ziilayibrari ze-MGI kwiilabhoratri zethu ukuze zilandelelaniswe kwi-DNBSEQ-T7, nto leyo evumela ukuba kubekho idatha ephezulu ngeendleko eziphantsi.
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Iilayibrari ezenziwe kwangaphambili ze-Illumina
Itekhnoloji yokulandelelanisa i-Illumina, esekelwe kwi-Sequencing by Synthesis (SBS), yindlela entsha ye-NGS eyamkelweyo kwihlabathi liphela, enoxanduva lokuvelisa ngaphezulu kwe-90% yedatha yokulandelelanisa yehlabathi. Umgaqo we-SBS ubandakanya ukufota ii-terminators eziguquguqukayo ezibhalwe nge-fluorescent njengoko i-dNTP nganye yongezwa, kwaye emva koko ziqhekeke ukuvumela ukufakwa kwesiseko esilandelayo. Njengoko zonke ii-dNTP ezine eziguqulweyo ze-terminator-bound zikhona kumjikelo ngamnye wokulandelelanisa, ukhuphiswano lwendalo lunciphisa ukukhetha kokufakwa. Le teknoloji iguquguqukayo ixhasa zombini iilayibrari ezifundwayo kunye nezimbini, ilungiselela uluhlu lwezicelo ze-genomic. Amandla aphezulu okusebenzisa i-Illumina sequencing kunye nokuchaneka kwayo kuyibeka njengesiseko sophando lwe-genomics, inika amandla izazinzulu ukuba zityhile ubunzima bee-genomes ngeenkcukacha ezingenakuthelekiswa nanto kunye nokusebenza kakuhle.
Inkonzo yethu yokulandelelanisa iilayibrari eyenziwe kwangaphambili ivumela abathengi ukuba balungiselele iilayibrari zokulandelelanisa ezivela kwimithombo eyahlukeneyo (i-mRNA, i-genome epheleleyo, i-amplicon, iilayibrari ezili-10x, phakathi kwezinye). Emva koko, ezi layibrari zingathunyelwa kumaziko ethu okulandelelanisa ukuze kulawulwe umgangatho kunye nokulandelelanisa kwiiplatifomu ze-Illumina.
