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Illumina kunye ne-BGI
Itekhnoloji yolandelelwano ye-Illumina, esekwe kulandelelwano nge-Synthesis (SBS), yinto entsha ye-NGS eyamkelwe kwihlabathi jikelele, enoxanduva lokuvelisa ngaphezulu kwe-90% yedatha yolandelelwano lwehlabathi.Umthetho-siseko we-SBS ubandakanya umboniso we-fluorescent obhalwe izitermina ezibuyiselwa umva njengoko i-dNTP nganye yongezwa, kwaye emva koko yacandwa ukuvumela ukufakwa kwesiseko esilandelayo.Ngazo zone ii-dNTP ezine-terminator-bound bounded dNTP ezikhoyo kumjikelo ngamnye wolandelelwano, ukhuphiswano lwendalo lunciphisa ukubandakanywa kokubandakanya.Ubuchwephesha obuguquguqukayo buxhasa amathala eencwadi afundwe kunye kunye nesiphelo sesibini, silungiselela uluhlu lwezicelo ze-genomic.I-Illumina yokulandelanisa i-high-throughput i-high-throughput ibeka njengelitye lembombo kuphando lwe-genomics, ixhobisa izazinzulu ukuba ziveze ubucukubhede be-genomes ngeenkcukacha ezingenakulinganiswa kunye nokusebenza kakuhle.
I-DNBSEQ, ephuhliswe yi-BGI, yenye itekhnoloji ye-NGS ekwaziyo ukuhla ngakumbi kwiindleko zolandelelwano kunye nokunyusa umthamo.Ukulungiswa kweelayibrari ze-DNBSEQ kubandakanya ukuhlukana kwe-DNA, ukulungiswa kwe-ssDNA kunye nokukhulisa isangqa esijikelezayo ukufumana i-nanoballs ye-DNA (DNB).Ezi ke ziye zilayishwe kumphezulu oqinileyo kwaye emva koko zilandelelaniswe ngokudityaniswa kweProbe-Anchor Synthesis (cPAS).
Inkonzo yethu yolandelelwano yethala leencwadi eyenziwe kwangaphambili iququzelela abathengi ekulungiseleleni iilayibrari zabo ezilandelelanayo ezivela kwimithombo eyahlukeneyo (mRNA, genome yonke, amplicon, phakathi kwabanye).Emva koko, la mathala eencwadi anokuthunyelwa kumaziko ethu okulandelelanisa ulawulo lomgangatho kunye nokulandelelana kwiiplatifti ze-Illumina okanye ze-BGI.
