条形 isibhengezo-03

Iimveliso

Ukuncitshiswa kwe-bisulfite ukulandelelana (i-RRBs)

84

Ukuncitshiswa kwe-bisulfite ukulandelelana (i-RRBs) iye yavela njengenye indlela esebenzayo nefanelekileyo kwi-genome genome genome bisulfite Ngelixa ii-WGBs zibonelela ngokuqonda ngokubanzi ngokuhlolisisa yonke i-genome kwisigqibo esisesikweni esinye, iindleko zayo eziphezulu zinokuba yinto ethintelayo. I-RRBs ithobela ngobuchule lo mngeni ngokukhetha uhlalutya inxalenye ye-genome. Le ndlela ixhomekeke ekuphuculeni imimandla ye-CPG yeSiqithi ye-CPG yeSiqithi ngu-MSPI Cleavivage ilandelwa kukhetho lwe-200-500 / 600 BPS. Ngenxa yoko, kuphela imimandla edlulisela kwi-CPG Islands ilandelelana, ngelixa ezo zikwiziqithi ezikude ze-CPG azifakwanga kuhlalutyo. Le nkqubo, idityaniswe ne-bisulfite ye-bisulfite, ivumela ukufunyanwa kwesigqibo esiphezulu seDNA, kunye nendlela yokulandelelana, i-PE150, igxile ikakhulu kwiziphelo zeflogion kunendlela ye-methyction. I-RRBs sisixhobo esibalulekileyo esenza uphando lwe-DNA ye-methylity ye-DNA ye-methyytives kunye nokuxhasa ulwazi lwe-epigenenetiic.


Iinkcukacha zenkonzo

I-Bioinformatics

Dumbulula

Ushicilelo olubonisiweyo

Iimpawu zenkonzo

● Ufuna ireferensi i-genome.

● I-DNA ye-ixane isetyenziselwa ukubeka esweni ukusebenza kwe-basiulfite.

● I-MSPI yokugaya ukusebenza ikwajongwa kwakhona.

● I-enzyme ephindwe kabini kwiisampulu zezityalo.

● Ukulandelelana kwi-Ilimina Novaq.

Izibonelelo zeNkonzo

Inye indlela esebenzayo nefanelekileyo kwii-wgbs: Ukwenza ukuba uhlalutyo luqhutywa ngendleko eziphantsi kwaye neemfuno ezisezantsi zesampulu.

Gcwalisa iqonga:Nika inkonzo yokumisa enye ebalaseleyo kulungiso lwesampulu, ulwakhiwo lwelayibrari, kunye nokulandelelana kuhlalutyo lwe-biinorinformatics.

Ubuchule obubanzi: Ngeeprojekthi zokulandelelana kwe-RRBs zigqitywe ngempumelelo kuluhlu oluqhelekileyo lweentlobo, i-Bmkgene izisa ngaphezulu kweshumi leminyaka, iqela lohlalutyo olupheleleyo, kunye nenkxaso epheleleyo yentengiso.

Inkcazo yenkonzo

Ithala leencwadi

Isicwangciso sokulandelelana

Imveliso yedatha ecetyiswayo

Ulawulo lwemeko

Ithamsanqa le-MSPI kunye neLasulfite Library

I-Mulmina Pe150

I-8 GB

I-Q30 ≥ 85%

Ukuguqulwa kwe-basulfite> 99%

I-MSPI yokusika ukusebenza kakuhle> 95%

Iifoto zeSampula

 

Uxinzelelo (nge / μl)

Inani lilonke (μg)

 

I-DNAIC ye-DNA

≥ 30

≥ 1

Ukonakaliswa okulinganiselweyo okanye ungcoliseko

Ukuhamba kwenkonzo

Ukuhanjiswa kwesampula

Ukuhanjiswa kwesampula

Ukulungiswa kwethala leencwadi

Ulwakhiwo lwethala leencwadi

Ukulandelelana

Ukulandelelana

Uhlalutyo lwedatha

Uhlalutyo lwedatha

Emva kokuthengisa iinkonzo

Iinkonzo zentengiso emva


  • Ngaphambili:
  • Okulandelayo:

  • 85

    Kubandakanya nohlalutyo olulandelayo:

    ● Ukulandelela komgangatho osemgangathweni;

    ● Imephu kwireferensi ye-genome;

    ● Ukufunyanwa kweziseko ze-5MC ze-5Mc methylates kunye nokuchongwa kwe-Motif;

    ● Uhlalutyo loKusasazwa kweMethylity kunye neSampulu;

    ● Uhlalutyo lwemimandla eyahlukileyo ye-methyty (i-DMrs);

    ● Ukunyanzeliswa kokunyanzelwa kweemfuza ezinxulumene ne-DMRS.

    Ulawulo loMgangatho: Ukugaya ukusebenza ngempumelelo (kwi-genome yemephu)

     

    86

     

    Ulawulo loMgangatho: Ukuguqulwa kwe-bisulfite (kwi-MethyIst yolwazi)

     

    87

     

    Imephu ye-methyyration: 5mc methytrass genome-ngokubanzi

    88

     

    Uthelekiso lweSampulu: Uhlalutyo lwecandelo eziphambili

     

    图片 89

     

    Uhlalutyo olwahlukileyo lwe-methy

     

    图片 90

     

     

    Jonga iNdawo yoPhando eye yaququzelelwa yinkonzo ye-BMKgene ye-BMKgene ye-Bisulfite ngengqokelela yeempapasho ezinyanyelweyo.

    Li, z. et al. .PNA NEXUS, 1 (4). I-DoI: 10.1093 / PNASnexus / pgac179.

    Tian, ​​H. ET Al. .Ijenali yaseYurophu yoPhando lweKlinikhi, 53 (11), iphe. I-E14055. Doi: 10.1111 / i-ECI.14055.

    Wu, y. et al. .Ijenali yoPhando lwe-Endorcrinoinoinologion, (12), iphe. 2365-2376. DoI: 10.1007 / S40618-022-018788.

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