● Ufuna ireferensi i-genome.
● I-DNA ye-ixane isetyenziselwa ukubeka esweni ukusebenza kwe-basiulfite.
● I-MSPI yokugaya ukusebenza ikwajongwa kwakhona.
● I-enzyme ephindwe kabini kwiisampulu zezityalo.
● Ukulandelelana kwi-Ilimina Novaq.
●Inye indlela esebenzayo nefanelekileyo kwii-wgbs: Ukwenza ukuba uhlalutyo luqhutywa ngendleko eziphantsi kwaye neemfuno ezisezantsi zesampulu.
●Gcwalisa iqonga:Nika inkonzo yokumisa enye ebalaseleyo kulungiso lwesampulu, ulwakhiwo lwelayibrari, kunye nokulandelelana kuhlalutyo lwe-biinorinformatics.
●Ubuchule obubanzi: Ngeeprojekthi zokulandelelana kwe-RRBs zigqitywe ngempumelelo kuluhlu oluqhelekileyo lweentlobo, i-Bmkgene izisa ngaphezulu kweshumi leminyaka, iqela lohlalutyo olupheleleyo, kunye nenkxaso epheleleyo yentengiso.
Ithala leencwadi | Isicwangciso sokulandelelana | Imveliso yedatha ecetyiswayo | Ulawulo lwemeko |
Ithamsanqa le-MSPI kunye neLasulfite Library | I-Mulmina Pe150 | I-8 GB | I-Q30 ≥ 85% Ukuguqulwa kwe-basulfite> 99% I-MSPI yokusika ukusebenza kakuhle> 95% |
Uxinzelelo (nge / μl) | Inani lilonke (μg) |
| |
I-DNAIC ye-DNA | ≥ 30 | ≥ 1 | Ukonakaliswa okulinganiselweyo okanye ungcoliseko |
Kubandakanya nohlalutyo olulandelayo:
● Ukulandelela komgangatho osemgangathweni;
● Imephu kwireferensi ye-genome;
● Ukufunyanwa kweziseko ze-5MC ze-5Mc methylates kunye nokuchongwa kwe-Motif;
● Uhlalutyo loKusasazwa kweMethylity kunye neSampulu;
● Uhlalutyo lwemimandla eyahlukileyo ye-methyty (i-DMrs);
● Ukunyanzeliswa kokunyanzelwa kweemfuza ezinxulumene ne-DMRS.
Ulawulo loMgangatho: Ukugaya ukusebenza ngempumelelo (kwi-genome yemephu)
Ulawulo loMgangatho: Ukuguqulwa kwe-bisulfite (kwi-MethyIst yolwazi)
Imephu ye-methyyration: 5mc methytrass genome-ngokubanzi
Uthelekiso lweSampulu: Uhlalutyo lwecandelo eziphambili
Uhlalutyo olwahlukileyo lwe-methy
Jonga iNdawo yoPhando eye yaququzelelwa yinkonzo ye-BMKgene ye-BMKgene ye-Bisulfite ngengqokelela yeempapasho ezinyanyelweyo.
Li, z. et al. .PNA NEXUS, 1 (4). I-DoI: 10.1093 / PNASnexus / pgac179.
Tian, H. ET Al. .Ijenali yaseYurophu yoPhando lweKlinikhi, 53 (11), iphe. I-E14055. Doi: 10.1111 / i-ECI.14055.
Wu, y. et al. .Ijenali yoPhando lwe-Endorcrinoinoinologion, (12), iphe. 2365-2376. DoI: 10.1007 / S40618-022-018788.