BMKCloud Log in

Iimveliso

Umekelo oluNcitshisiweyo lweBisulfite Sequencing (RRBS)

UMelo oNcitshisiweyo we-Bisulfite Sequencing (RRBS) Umfanekiso obonakalisiweyo

Uphando lwe-DNA methylation luhlala luyisihloko esishushu kuphando lwezifo, kwaye luhambelana ngokusondeleyo ne-gene expression kunye ne-pheno-typic traits.I-RRBS yindlela echanekileyo, esebenzayo kunye neyoqoqosho yophando lwe-DNA methylation.Ukutyetyiswa komgqugquzeli kunye nemimandla yesiqithi se-CpG nge-enzymatic cleavage (Msp I), edityaniswe nokulandelelana kwe-Bisulfite, ibonelela ngesisombululo esiphezulu se-DNA methylation.

Iqonga: I-Illumina NovaSeq Platform

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Iziphumo zeDemo

IiNgcaciso zeNkonzo

Iqonga: iqonga leNovaSeq, PE150

Uhlobo lwethala leencwadi: 200-400bp faka i-bisulfite enyangwa kwithala leencwadi le-DNA

Isicwangciso sokulandelelana: I-Paired-End 150 bp

Cebisa imveliso yedatha: 10 Gb idatha ekrwada / isampuli

IiMfuno zeSampulu

DNA ubuninzi: ≥ 2ug

Ugxininiso lweDNA:≥ 20ng/μl.

Ubunyulu: OD260/280 = 1.8 ukuya ku-2.0 ngaphandle kokuthotywa okanye ukungcoliswa kwe-RNA

Uhlalutyo lwe-Bioinformatics

umfanekiso weskrini 4-03

Ukuhamba komsebenzi weNkonzo

ukuhanjiswa kwesampuli

Ukuhanjiswa kwesampuli

ULungiselelo lweThala leeNcwadi

Ukwakhiwa kwethala leencwadi

Ukulandelelanisa

Ukulandelelanisa

Uhlalutyo lwedatha

Uhlalutyo lwedatha

Emva kweeNkonzo zentengiso

Iinkonzo emva kokuthengisa

Ngaphambili: Prokaryotic RNA ulandelelwano

Okulandelayo: I-PacBio-ubude obugcweleyo be-Transcriptome (Non-Reference)

a.Iinkcukacha-manani zolungelelwaniso lwe-genome

Ubalo lwemephu lufundeka

SIzibalo zobunzulu bolandelelwano kunye nokugubungela indawo yeC

Aukhuselo oluqokelelweyo

Dukusebenza kakuhle kwesampulu nganye

b.Ukufunyanwa kwendawo ye-methylation

Uluhlu lwamanqanaba e-methylation kwindawo ye-C

BS ukuguqulwa

Methylated cytosine

Ukuhanjiswa kwenqanaba le-methylation

c.Imephu ye-methylation

Genome-banzi

Genome element

d.Ukuthelekiswa kwamanqanaba e-methylation phakathi kweesampuli

Pemoyeni Unxulumano lwe-CG methylation

PI-CA-CG methylation

CUkukhanya kwe-CG methylation

d.Uhlalutyo lwe-methylation ngokwahlukileyo

SIinkcukacha-manani zeDMR

Ukuhanjiswa kobude be-DMR

Hyitya imephu

DUkusasazwa kwe-MR kwindawo ekujoliswe kuyo

fumana isicatshulwa

Bhala umyalezo wakho apha kwaye uwuthumele kuthi

Imvelisoiindidi

Thumela umyalezo wakho kuthi: