I-RNA encinci Ukulandelelana-Illumina
Iimbonakalo
● Ukulungiswa kwethala leencwadi lokukhetha ubukhulu.
● Uhlalutyo lwe-Bioinformatic lugxile kuqikelelo lwe-miRNA kunye nezinto ekujoliswe kuzo.
Iinkonzo eziluncedo
●UbuGcisa obuBanzi: Senze iisampulu ezingaphezulu kwama-300, ezithatha iintlobo ngeentlobo zeesampulu. Sizisa ubutyebi bobuchule kwiprojekthi nganye.
●Ulawulo loMgangatho olungqongqo: Sisebenzisa iindawo zolawulo ezingundoqo kuzo zonke izigaba, ukusuka kulungiselelo lwesampulu ukuya kulungiselelo lwethala leencwadi, ulandelelwano kunye ne-bioinformatics. Ukubeka iliso kwethu ngononophelo kuqinisekisa ukuhanjiswa kweziphumo ezihlala zikumgangatho ophezulu.
●Uhlalutyo olubanzi lwe-bioinformatics:Ivumela ukuchongwa kwee-miRNA zombini ezaziwayo kunye neenoveli, ukuchongwa kweethagethi ze-miRNAs, kunye nochazo olusebenzayo oluhambelanayo kunye nokutyebisa ngoovimba beenkcukacha ezininzi (KEGG, GO).
●Inkxaso yePost-Sales: Siyayiqonda indlela okubaluleke ngayo ukubakho, yiyo loo nto ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi ngeenyanga ezi-3 emva kokuthengiswa kwenkonzo. Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.
IiMfuno zeSampulu kunye nokuhanjiswa
| Ithala leencwadi | Iqonga | Idatha ecetyiswayo | Idatha ye-QC |
| Ubungakanani obukhethiweyo | Illumina SE50 | I-10M-20M ifundeka | Q30≥85% |
IiMfuno zeSampulu:
IiNucleotides:
| Conc.(ng/μl) | Imali (μg) | Ubunyulu | Ingqibelelo |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Unyino okanye akukho protein okanye ungcoliseko lweDNA olubonakaliswe kwijeli. | I-RIN≥6.0; 5.0≥28S/18S≥1.0; umphakamo osisiseko okanye ongekhoyo |
● Izityalo:
Ingcambu, Isiqu okanye iPetal: 450 mg
Igqabi okanye Imbewu: 300 mg
Isiqhamo: 1.2 g
● Isilwanyana:
Intliziyo okanye Amathumbu: 450 mg
Viscera okanye Brain: 240 mg
Izihlunu: 600 mg
Amathambo, Iinwele okanye Ulusu: 1.5g
● IiArthropods:
Izinambuzane: 9g
I-crustacea: 450 mg
● Igazi elipheleleyo: 2 iityhubhu
● Iiseli: 106 iiseli
● ISerum nePlasma:6 ml
Unikezelo lweSampuli eCetyisiweyo
Isikhongozeli: 2 ml centrifuge ityhubhu (Ifoyile yeTin ayikhuthazwa)
Isampuli yokuleyibhela: Iqela+liphindaphinda umz. A1, A2, A3; B1, B2, B3.
Ukuthunyelwa ngenqanawa:
1. Umkhenkce owomileyo: Iisampulu kufuneka zipakishwe kwiingxowa kwaye zingcwatywe kumkhenkce owomileyo.
2. Iityhubhu ze-RNAstable: Iisampulu ze-RNA zingomiswa kwi-tube ye-RNA yokuzinzisa (umzekelo, i-RNAstable®) kwaye ithunyelwe kwiqondo lokushisa.
Ukuhamba komsebenzi weNkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampuli
Ukukhutshwa kwe-RNA
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
Bioinformatics
● Ulawulo lomgangatho wedatha ekrwada
● Ukuhlelwa kweRNA encinci
● Ukuchazwa kwe-miRNA kunye nohlalutyo lwentetho eyahlukileyo
● I-MiRNA yejini ekujoliswe kuyo kunye ne-miRNA echazwe ngokwahlukileyo yegene yochazo
● Inkcazo yemfuza ekujoliswe kuyo kunye nokuchazwa ngokwahlukileyo kokwenziwa komsebenzi wemfuza we-miRNA
Ukuchongwa kwe-miRNA: isakhiwo kunye nobunzulu
Ukubonakaliswa okwahlukileyo kwe-miRNA - ukuhlanganisana kwe-hiearchical
Inkcazo esebenzayo yethagethi yee-miRNA ezichazwe ngokwahlukileyo
Phonononga ukuqhubela phambili kophando oluququzelelwa yi-BMKGene' sRNA iinkonzo zolandelelwano ngengqokelela egciniweyo yopapasho.
Chen, H. et al. (2023) 'Usulelo lwentsholongwane lunqanda i-saponin biosynthesis kunye ne-photosynthesis kwiPanax notoginseng',IPhysiology yezityalo kunye neBiochemistry, 203, iphe. 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al. (2023) 'Isityalo se-FYVE-esineprotheyini ye-FREE1 edibanisa nezinto ezincinci zokucinezela i-miRNA biogenesis',Iingxelo ze-EMBO, 24(1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. et al. (2023) 'I-MicroRNA Ame-Bantam-3p Ilawula uPhuhliso lweLarval Pupal ngokuJonga i-Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) kwi-Honeybee, i-Apis mellifera',Ijenali yaMazwe ngaMazwe yeNzululwazi yeMolekyuli, 24(6), iphe. 5726. doi: 10.3390/IJMS24065726/S1.
UZhang, M. et al. (2018) 'Uhlalutyo oludibeneyo lwe-MiRNA kunye neGene eNxulunyaniswe noMgangatho weNyama ibonisa ukuba i-Gga-MiR-140-5p ichaphazela i-Intramuscular Fat Deposition in Chickens',IFiziyoloji yeSelula kunye neBiochemistry, 46(6), iphepha 2421–2433. doi: 10.1159/000489649.
