BMKCloud Log in
条形 ibhanile-03

Iimveliso

I-RNA encinci yokulandelelana-Illumina

Iimolekyuli ezincinci ze-RNA (sRNA), ngokuqhelekileyo phantsi kwe-nucleotides ye-200 ubude, zibandakanya i-microRNAs (miRNAs), i-RNAs encinci ephazamisayo (siRNAs), kunye ne-piwi-interacting RNAs (piRNAs).Phakathi kwezi, ii-miRNAs, malunga ne-20-24 nucleotides ubude, ziphawuleka ngakumbi kwiindima zazo zolawulo olubalulekileyo kwiinkqubo ezahlukeneyo zeselula.Ngeepateni zokuchaza ezithe ngqo kunye nenqanaba elithile, ii-miRNAs zibonisa ulondolozo oluphezulu kuzo zonke iintlobo ezahlukeneyo.

Iqonga: Illumina NovaSeq


Iinkcukacha zeNkonzo

Bioinformatics

Iziphumo zeDemo

Iimpapasho Ezikhoyo

Iimbonakalo

● Ubungakanani bokukhetha i-RNA phambi kokulungiswa kwethala leencwadi

● Uhlalutyo lwe-Bioinformatic lugxile kuqikelelo lwe-miRNA kunye nezinto ekujoliswe kuzo

Iinkonzo eziluncedo

Uhlalutyo olubanzi lwe-bioinformatics:ukwenza ukuba kuchongwe zombini ii-miRNAs ezaziwayo kunye neenoveli, ukuchongwa kweethagethi ze-miRNAs kunye nochazo olusebenzayo oluhambelanayo kunye nokutyebisa ngoovimba abaninzi (KEGG, GO)

Ulawulo loMgangatho olungqongqo: siphumeza iindawo zolawulo ezingundoqo kuzo zonke izigaba, ukusuka kwisampulu kunye nolungiselelo lwethala leencwadi ukuya kulandelelwano kunye ne-bioinformatics.Olu hlolo lucokisekileyo luqinisekisa ukuhanjiswa kweziphumo ezihlala zikumgangatho ophezulu.

Inkxaso yePost-Sales: Ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye neenyanga ezi-3 emva kokuthengiswa kwenkonzo.Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.

UbuGcisa obuBanzi: ngerekhodi yokuvala ngempumelelo iiprojekthi ezininzi ze-sRNA ezigubungela iintlobo ezingaphezu kwe-100 kwiindawo ezahlukeneyo zophando, iqela lethu lizisa ubutyebi bamava kwiprojekthi nganye.

IiMfuno zeSampulu kunye nokuhanjiswa

Ithala leencwadi

Iqonga

Idatha ecetyiswayo

Idatha ye-QC

Ubungakanani obukhethiweyo

Illumina SE50

I-10M-20M ifundeka

Q30≥85%

IiMfuno zeSampulu:

IiNucleotides:

Conc.(ng/μl)

Imali (μg)

Ubunyulu

Ingqibelelo

≥ 80

≥ 0.5

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Unyino okanye akukho protein okanye ungcoliseko lweDNA olubonakaliswe kwijeli.

I-RIN≥6.5;

5.0≥28S/18S≥1.0;

umphakamo osisiseko okanye ongekhoyo

● Izityalo:

Ingcambu, Isiqu okanye iPetal: 450 mg

Igqabi okanye Imbewu: 300 mg

Isiqhamo: 1.2 g

● Isilwanyana:

Intliziyo okanye Amathumbu: 450 mg

Viscera okanye Brain: 240 mg

Izihlunu: 600 mg

Amathambo, Iinwele okanye Ulusu: 1.5g

● IiArthropods:

Izinambuzane: 9g

I-crustacea: 450 mg

● Igazi elipheleleyo: 2 iityhubhu

● Iiseli: 106 iiseli

● ISerum nePlasma:6 ml

Unikezelo lweSampuli eCetyisiweyo

Isikhongozeli:
I-2 ml yetyhubhu ye-centrifuge (i-tin foil ayikhuthazwa)
Isampuli yokuleyibhela: Iqela+liphindaphinda umz. A1, A2, A3;B1, B2, B3... ...

Ukuthunyelwa ngenqanawa:
1.Umkhenkce owomileyo: Iisampuli kufuneka zipakishwe kwiingxowa kwaye zingcwatywe kwindawo eyomileyo.
I-2.RNAstable tubes: Iisampulu ze-RNA zingomiswa kwi-tube yokuzinzisa i-RNA (umzekelo, i-RNAstable®) kwaye ithunyelwe kwiqondo lokushisa.

Ukuhamba komsebenzi weNkonzo

Isampulu ye-QC

Uyilo lovavanyo

ukuhanjiswa kwesampuli

Ukuhanjiswa kwesampuli

Ulingo lokulinga

Ukukhutshwa kwe-RNA

ULungiselelo lweThala leeNcwadi

Ukwakhiwa kwethala leencwadi

Ukulandelelanisa

Ukulandelelanisa

Uhlalutyo lwedatha

Uhlalutyo lwedatha

Emva kweeNkonzo zentengiso

Iinkonzo emva kokuthengisa


  • Ngaphambili:
  • Okulandelayo:

  • Bioinformatics

    wps_doc_14

    Ukuchongwa kwe-miRNA: isakhiwo kunye nobunzulu

     

     

     I-miRNA-isandulela-isakhiwo-kunye-nolandelelwano-bunzulu

     

    Ukubonakaliswa okwahlukileyo kwe-miRNA - ukuhlanganisana kwe-hiearchical

    Umfanekiso we34

     

    Inkcazo esebenzayo yethagethi yee-miRNA ezichazwe ngokwahlukileyo

    图片35

    Phonononga ukuqhubela phambili kophando oluququzelelwa yi-BMKGene' sRNA iinkonzo zolandelelwano ngengqokelela egciniweyo yopapasho.

      

    Chen, H. et al.(2023) 'Izifo zentsholongwane zivimbela i-saponin biosynthesis kunye ne-photosynthesis kwi-Panax notoginseng', i-Plant Physiology kunye ne-Biochemistry, i-203, iphe.108038. doi: 10.1016/J.PLAPHY.2023.108038.

    Li, H. et al.(2023) 'Isityalo se-FYVE esineprotheyini ye-FREE1 enxulumana namalungu e-microprocessor ukucinezela i-miRNA biogenesis', iingxelo ze-EMBO, 24(1).doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.

    Yu, J. et al.(2023) 'I-MicroRNA Ame-Bantam-3p iLawula uPhuhliso lweLarval Pupal ngokuJonga i-Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) kwi-Honeybee, i-Apis mellifera', i-International Journal ye-Molecular Sciences, 24(6), p. .5726. doi: 10.3390/IJMS24065726/S1.

    UZhang, M. et al.(2018) 'Uhlalutyo oludityanisiweyo lwe-MiRNA kunye neGene eNxulunyaniswe noMgangatho weNyama Ibonisa ukuba i-Gga-MiR-140-5p ichaphazela i-Intramuscular Fat Deposition kwiiNkukhu', i-Cellular Physiology kunye ne-Biochemistry, i-46 (6), iphe. 2421-2433.doi: 10.1159/000489649.

    fumana isicatshulwa

    Bhala umyalezo wakho apha kwaye uwuthumele kuthi

    Thumela umyalezo wakho kuthi: