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Iimveliso

Ikhithi yokuCoca i-DNA ehlakaniphileyo ye-TGuide

Umfanekiso obonisiweyo weKit yokuHlambulula i-TGuide Smart DNA

Ifumana i-DNA esemgangathweni ophezulu kwimveliso ye-PCR okanye kwiijeli ze-agarose.

Qhagamshelana nathi

Iinkcukacha zeNkonzo

Inombolo yekhathalogu / ukupakisha

Hayi ikati	ID	Inani lamalungiselelo
4993550 (ikhatriji)	I-DP642-DE	48

Isikhokelo sokukhetha i-reagent ye-TGuide S16

I-Nuclei Acid	Uhlobo lweSampuli	Igama lemveliso	Ubungakanani bokupakisha (ukulungiselela) Ikhatriji/ ipleyiti	Inombolo yekati. Ikhatriji/ipleyiti
i-DNA	Izicubu zezilwanyana	I-TGuide Smart Magnetic Tissue DNA Kit	48/96	4993547/4995038
i-DNA	Isityalo kunye neMbewu	I-TGuide Smart Magnetic Plant DNA Kit	48	4993548
i-DNA	Umhlaba/Isitulo	I-TGuide Smart Soil/Stool DNA Kit	48	4993549
i-DNA	Iimveliso zeGel kunye nePCR	Ikhithi yokuCoca i-DNA ehlakaniphileyo ye-TGuide	48	4993550
i-DNA	Igazi Elipheleleyo	I-TGuide Smart Blood Genomic DNA Kit	48/96	4993703/4995206
i-DNA	Amabala eSeli/eSwab/omileyo, njl.	I-TGuide Smart Universal DNA Kit	48/96	4993704/4995040
I-RNA	Igazi/Iseli/Izicubu	I-TGuide Smart Blood/Cell/Tissue RNA Kit	48/96	4993551/4995043
I-RNA	Isityalo kunye neMbewu	I-TGuide Smart Magnetic Plant RNA Kit	48	4993552
I-DNA/RNA	Ii-Fluids ezingenamaSeli (i-Serum, njl.njl.)	I-TGuide Smart Viral DNA/RNA Kit	48/96	4993702/4995207

Intshayelelo yemveliso

Ifumana i-DNA ngokukhawuleza nangokufanelekileyo kwijeli ye-TAE/TBE kunye nemveliso ye-PCR.

Uluhlu lobungakanani besiqwenga se-DNA: 100 bp-15 kb.

I-DNA efunyenwe ngokusebenzisa le khithi ingasetyenziselwa ukugaya ukutya, i-PCR, ulandelelwano, ukuvavanya ilayibrari, ukubopha, ukuguqulwa kunye nezinye iimvavanyo.

Iimbonakalo

Kulula kakhulu ukuyisebenzisa

Akukho msebenzi wongezelelweyo wokufaka imibhobho.

Umthamo we-elution ungalungiswa njengoko kufuneka.

Ukufakela nokusebenza kufuna uqeqesho oluncinci. Ngeenkqubo ezifakwe kwangaphambili, khupha ikhatriji, khetha iprotocol kwaye usebenzise uvavanyo lwakho. Iinkqubo zilungele ukusetyenziswa, kwaye ziyakwazi ukuhlengahlengiswa.

Ii-reagents ezilayishwe kwangaphambili kunye nezinto ezisetyenziswayo ezilahlwayo ezifanayo.

Ukuphathwa kweekhemikhali kuyasuswa kakhulu ukuqinisekisa ukhuseleko lokusebenza.

Isiphumo seDemo

Ukucocwa nokubuyiselwa kweDNA ye-agarose gel eyi-300 bp
Uxinzelelo lwejeli ye-Agarose :1.5%(TBE)
Iyalayisha
umthamo: 8 μl
Uphawu: 50 bp ileli ye-DNA, iTIANGEN
I-S16-01, i-S16-02, kunye ne-S16-03 zimele uvavanyo lokucoca nokubuyisela isampuli oluhambelanayo kusetyenziswa ii-3 TGuide S16 Nucleic Acid Extractors ezine-TGuide Smart DNA Purification Kit (4993550) ngaxeshanye.
Inkampani: ikhithi yokukhupha iikholamu ezijikelezayo ngesandla yophawu oludumileyo

Ukucocwa nokubuyiselwa kweDNA ye-agarose segment ye-2000 bp
Uxinzelelo lwejeli ye-Agarose:1.5% (TBE)
Umthamo wokulayisha: 8 μl
Uphawu: D2000, TIANGEN
I-S16-01, i-S16-02, kunye ne-S16-03 zimele ukucocwa kunye nokubuyiselwa kwesampuli ngaxeshanye
zama ukusebenzisa ii-TGuide S16 Nucleic Acid Extractors ezi-3 ezine-TGuide Smart DNA Purification Kit (4993550) ngaxeshanye.
Inkampani: ikhithi yokukhupha iikholamu ezijikelezayo ngesandla yophawu oludumileyo

Isiphumo sovavanyo: ukusebenzisa i-TGuide S16 Nucleic Acid Extractor enekhithi ekhethekileyo i-TGuide Smart DNA Purification Kit (4993550) kunokufezekisa ukwenziwa ngokuzenzekelayo kokubuyiswa kwejeli. Ukuhambelana kokuhlanjululwa nokubuyiselwa kwayo kugqwesile, kwaye isiphumo sokukhupha singcono kuneemveliso ezikhuphisanayo zezinye iinkampani.

Ukubuyiswa kwe-DNA ye-15000 bp section agarose gel kunye nokucocwa kwemveliso ye-PCR
Uxinzelelo lwejeli ye-Agarose :1% (TBE)
Umthamo wokulayisha: 3 μl
Uphawu:
D15000,TIANGEN
G1, G2: ukubuyiswa kwejeli ye-agarose
C1: Ukucocwa kwemveliso ye-PCR.
Iprotokholi eyenziwe ngesandla: ikhithi yokukhupha i-spin-column ngesandla yophawu oludumileyo lwe-B
Inkampani: ikhithi yokukhupha esekwe kwindlela yemagnethi yophawu oludumileyo

Iziphumo zovavanyo: kusetyenziswa i-TGuide S16 Nucleic Acid Extractor enekhithi ye-TGuide Smart DNA Purification Kit (4993550), ukubuyiswa kwejeli ye-agarose kunye nokucocwa kwemveliso ye-PCR kunokufezekiswa ngokuzenzekelayo, kwaye iziphumo zokucoca ziyafana nezo zokucoca okusekwe kwi-spin-column.