Ulandelelwano lwegenome bisulfite epheleleyo
Iinkonzo eziluncedo
● Iqonga elipheleleyo: ukubonelela ngenkonzo egqwesileyo kwindawo enye ukusuka kwisampula yokusetyenzwa, ukwakhiwa kwethala leencwadi, ulandelelwano ukuya kuhlalutyo lwe-bioinformatics.
● Ukuchaneka okuphezulu: Iteknoloji yokuguqulwa kwe-methylation ekhulileyo inokuhlalutya ngokuchanekileyo imeko ye-methylation yesiseko esisodwa se-C.
● Ukhuseleko olubanzi: ukufunyanwa kweendawo ze-methylation kwinqanaba le-genome-wide.
● Ukuphindaphinda okulungileyo: ukuqhutyelwa phambili okuzinzile, okufanelekileyo kuhlalutyo lokuthelekisa phakathi kweesampuli ezininzi.
IiNkcazelo zeSampule
|
Iqonga
|
Funda Ubude
|
Isicwangciso solandelelwano
|
| Illumina | PE 150 | I-30X, iikopi ezintathu zebhayoloji, ezinomgangatho ophezulu we-genome yesalathiso |
IiMfuno zeSampulu
|
Uhlobo lweSampuli
|
Imali (μg)
|
Conc.(ng/μl)
|
Umthamo (μl)
|
Ubunyulu
|
| gDNA | ≥ 2 | ≥ 20
| ≥ 20
| Ukuthotywa okulinganiselweyo kunye nokungcoliseka
|
Ukuhamba komsebenzi weNkonzo
Ukuhanjiswa kwesampuli
Ukutsalwa kwe-DNA
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Ukuhanjiswa kwedatha
Bioinformatics
1.I-Genome-wide DNA methylation distribution
I-2.Inkcazo kwingingqi ye-CGI ene-methylated kakhulu
