Ulandelelwano olupheleleyo lwe-transcriptome - Illumina
Iimbonakalo
● Ithala leencwadi elinamacala amabini ukuze lilandelelanise i-transcriptome epheleleyo: Ukuncipha kwe-rRNA kulandelwa yi-PE150 yolungiselelo lwethala leencwadi kunye nokukhethwa kobungakanani kulandelwa yi-SE50 yokulungiselela ithala leencwadi.
● Gqibezela uhlalutyo lwe-bioinformatics ye-mRNA, i-lncRNA, i-circRNA kunye ne-miRNA kwiingxelo ezahlukeneyo ze-bioinformatics.
● Uhlalutyo oludityanisiweyo lwazo zonke inkcazo ye-RNA kwingxelo edibeneyo, kubandakanywa uhlalutyo lwenethiwekhi ye-ceRNA.
Iinkonzo eziluncedo
●Uhlalutyo olunzulu lwamanethiwekhi olawulo: Uhlalutyo lwenethiwekhi ye-ceRNA lwenziwe lulandelelwano oludibeneyo lwe-mRNA, i-lncRNA, i-circRNA kunye ne-miRNA kunye nokuhamba komsebenzi we-bioinformamatic.
●Isichasiselo esiBanzi: sisebenzisa ii-database ezininzi ukuze sichaze ngokusebenzayo i-Differentially Expressed Genes (DEGs) kwaye senze uhlalutyo oluhambelanayo lokutyebisa, ukubonelela ngengqiqo kwiinkqubo zeselula kunye neemolekyuli eziphantsi kwempendulo ye-transcriptome.
●UbuGcisa obuBanzi: ngerekhodi lokuvala ngempumelelo ngaphezulu kwe-2000 yeeprojekthi ezipheleleyo ze-transcriptome kwindawo eyahlukeneyo yophando, iqela lethu lizisa ubutyebi bamava kwiprojekthi nganye.
●Ulawulo loMgangatho olungqongqo: siphumeza iindawo zolawulo ezingundoqo kuzo zonke izigaba, ukusuka kwisampulu kunye nolungiselelo lwethala leencwadi ukuya kulandelelwano kunye ne-bioinformatics.Olu hlolo lucokisekileyo luqinisekisa ukuhanjiswa kweziphumo ezihlala zikumgangatho ophezulu.
● Isihlomelo esiBanzi: sisebenzisa ii-database ezininzi ukuze sichaze ngokusebenzayo i-Differentially Expressed Genes (DEGs) kwaye senze uhlalutyo oluhambelanayo lokutyebisa, ukubonelela ngengqiqo kwiinkqubo zeselula kunye neemolekyuli eziphantsi kwempendulo ye-transcriptome.
●Inkxaso yePost-Sales: Ukuzinikela kwethu kudlulela ngaphaya kokugqitywa kweprojekthi kunye neenyanga ezi-3 emva kokuthengiswa kwenkonzo.Ngeli xesha, sinikezela ngokulandelelana kweprojekthi, uncedo lokusombulula ingxaki, kunye neeseshoni ze-Q&A ukujongana nayo nayiphi na imibuzo enxulumene neziphumo.
IiMfuno zeSampulu kunye nokuhanjiswa
| Ithala leencwadi | Isicwangciso sokulandelelana | Idatha iyacetyiswa | Ulawulo lwemeko |
| I-rRNA iphelile | Illumina PE150 | I-GB engama-16 | Q30≥85% |
| Ubungakanani obukhethiweyo | Illumina SE50 | I-10-20M ifundeka |
IiMfuno zeSampulu:
IiNucleotides:
| Conc.(ng/μl) | Imali (μg) | Ubunyulu | Ingqibelelo |
| ≥ 100 | ≥ 1 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Unyino okanye akukho protein okanye ungcoliseko lweDNA olubonakaliswe kwijeli. | Izityalo: RIN≥6.5 Isilwanyana: RIN≥7.0 5.0≥28S/18S≥1.0; umphakamo osisiseko okanye ongekhoyo |
Unikezelo lweSampuli eCetyisiweyo
Isikhongozeli:
I-2 ml yetyhubhu ye-centrifuge (i-tin foil ayikhuthazwa)
Isampuli yokuleyibhela: Iqela+liphindaphinda umz. A1, A2, A3;B1, B2, B3... ...
Ukuthunyelwa ngenqanawa:
1.Umkhenkce owomileyo: Iisampuli kufuneka zipakishwe kwiingxowa kwaye zingcwatywe kwindawo eyomileyo.
I-2.RNAstable tubes: Iisampulu ze-RNA zingomiswa kwi-tube yokuzinzisa i-RNA (umzekelo, i-RNAstable®) kwaye ithunyelwe kwiqondo lokushisa.
Ukuhamba komsebenzi weNkonzo
Uyilo lovavanyo
Ukuhanjiswa kwesampuli
Ukukhutshwa kwe-RNA
Ukwakhiwa kwethala leencwadi
Ukulandelelanisa
Uhlalutyo lwedatha
Iinkonzo emva kokuthengisa
Bioinformatics
RNA isishwankathelo sokubonisa
Iimfuza ezivezwe ngokwahlukileyo
Uhlalutyo lwe-ceRNA
Ii-miRNA ezichazwe ngokwahlukileyo kunye nee-RNA ezinxulumeneyo
Phonononga ukuqhubela phambili kophando oluququzelelwa yi-BMKGene 'yonke iinkonzo zolandelelwano lwe-transcriptome ngengqokelela egciniweyo yopapasho.
Dai, Y. et al.(2022) 'Iiprofayili zenkcazo ebanzi ye-mRNAs, i-lncRNAs kunye ne-miRNAs kwi-Kashin-Beck isifo esichongwe nge-RNA-sequencing', i-Molecular Omics, i-18 (2), iphe. 154-166.doi: 10.1039/D1MO00370D.
U-Liu, N. nan et al.(2022) 'Uhlalutyo olupheleleyo lwee-transcriptomes zokumelana nokubanda kwe-Apis cerana kwiNtaba yaseChangbai ngexesha lobusika.', Gene, 830, iphe. 146503-146503.doi: 10.1016/J.GENE.2022.146503.
Wang, XJ et al.(2022) 'I-Multi-Omics Integration-Based Prioritization of Competing Endogenous RNA Regulation Networks in Small Cell Lung Cancer: Iimpawu zeMolekyuli kunye naBagqatswa beziyobisi', iFrontiers kwi-Oncology, i-12, iphe.904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xu, P. et al.(2022) 'Uhlalutyo oludibeneyo lwe-lncRNA / circRNA-miRNA-mRNA inkcazo yeeprofayili ityhila ingqiqo yenoveli kwiindlela ezinokuthi ziphendule kwiingcambu ze-nematodes kwi-peanut', i-BMC Genomics, i-23 (1), iphepha 1-12.doi: 10.1186/S12864-022-08470-3/AMAFIGURES/7.
Yan, Z. et al.(2022) 'I-Whole-transcriptome yokulandelelana kwe-RNA igxininisa iindlela ze-molecular ezinxulumene nokugcinwa komgangatho we-postharvest kwi-broccoli nge-red LED irradiation', i-Postharvest Biology and Technology, i-188, iphe.111878. doi: 10.1016/J.POSTHARVBIO.2022.111878.
