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条形pasia-03

Awọn ọja

Awọn kokoro arun pipe Genome

Awọn imọ-ẹrọ Biomarker n pese iṣẹ ṣiṣe lẹsẹsẹ lori ṣiṣe jiini pipe ti kokoro arun pẹlu aafo odo.Ṣiṣan iṣẹ akọkọ ti awọn kokoro arun pipe iṣelọpọ jiini pẹlu tito lẹsẹsẹ iran kẹta, apejọ, asọye iṣẹ ṣiṣe ati itupalẹ bioinformatic ti ilọsiwaju ti nmu awọn ibi-afẹde iwadii kan pato ṣẹ.Ifitonileti okeerẹ diẹ sii ti jiini kokoro arun n fun ni agbara ṣiṣafihan ti awọn ilana ipilẹ ti o wa labẹ awọn ilana ti ibi wọn, eyiti o tun le pese itọkasi to niyelori fun awọn iwadii jiinidi ninu awọn eya eukaryotic ti o ga julọ.

Syeed:Nanopore PromethION P48 + Illumina NovaSeq Platform

PacBio Sequel II


Awọn alaye Iṣẹ

Awọn abajade Ririnkiri

Awọn anfani Iṣẹ

● Awọn ilana ilana ilana pupọ ti o wa fun awọn ibi-afẹde iwadii oriṣiriṣi

● Awọn kokoro arun pipe jiini pẹlu 0 Gap ẹri.

● Ni iriri giga ni apejọ genome kokoro arun pẹlu diẹ sii ju 10,000 ti o pejọ.

● Ọjọgbọn lẹhin-tita atilẹyin imọ-ẹrọ egbe nmu awọn ibeere iwadi pato diẹ sii.

Awọn pato Iṣẹ

TiteleIlana

Ile-ikawe

Didara ẹri

Ifoju-yika akoko

Nanopore 100X + Illumina 50X

Nanopore 20K

PE150

0 Aafo

30 Ọjọ

PacBio HiFi 30X

PacBio 10K

Awọn itupalẹ bioinformatics

● Aise data didara iṣakoso

● Apejọ Genome

● Ayẹwo paati Jinom

● Atọka iṣẹ Jiini

● Ayẹwo genomic afiwera

2

Awọn ibeere Ayẹwo ati Ifijiṣẹ

Awọn ibeere Apeere:

FunDNA ayokuro:

Apeere Iru

Iye

Ifojusi

Mimo

DNA ayokuro

2 μg

20ng/μl

OD260/280 = 1.6-2.5

Fun awọn ayẹwo iṣan:

Iru apẹẹrẹ Itọju ayẹwo ti a ṣe iṣeduro Ayẹwo ipamọ ati sowo
Awọn kokoro arun Ṣe akiyesi awọn kokoro arun labẹ maikirosikopu ki o gba awọn kokoro arun ni ipele ipari wọn

Gbigbe aṣa kokoro arun (ti o ni isunmọ. 3-4.5e9 awọn sẹẹli) sinu eppendorf 1.5 tabi 2 milimita.(Tẹ lori yinyin)

Centrifuge tube fun iṣẹju 1 ni 14000 g lati gba awọn kokoro arun ati yọọ supernatant ni pẹkipẹki.

Pa tube naa ki o di awọn kokoro arun sinu nitrogen olomi fun o kere 30 min.Tọju tube ni -80 ℃ firiji.

Di awọn ayẹwo naa sinu omi nitrogen fun awọn wakati 3-4 ati fipamọ sinu nitrogen olomi tabi iwọn -80 si ifiṣura igba pipẹ.Ayẹwo gbigbe pẹlu yinyin gbigbẹ ni a nilo.

Sisan Iṣẹ Iṣẹ

ifijiṣẹ ayẹwo

Ifijiṣẹ apẹẹrẹ

Igbaradi Library

Ikole ìkàwé

Titele

Titele

Itupalẹ data

Itupalẹ data

Lẹhin ti sale Services

Lẹhin-tita awọn iṣẹ


  • Ti tẹlẹ:
  • Itele:

  • 1.Circos ti kokoro arun genome

    3

    2.Coding pupọ asọtẹlẹ

    4

    3.Comparative genomics onínọmbà: phylogenetic igi

    5

    gba agbasọ

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