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Awọn ọja

Awọn kokoro arun pipe Genome

Awọn imọ-ẹrọ Biomarker n pese iṣẹ ṣiṣe lẹsẹsẹ lori kikọ jiini pipe ti kokoro arun pẹlu aafo odo.Ṣiṣan iṣẹ akọkọ ti awọn kokoro arun pipe iṣelọpọ jiini pẹlu tito lẹsẹsẹ iran kẹta, apejọ, asọye iṣẹ-ṣiṣe ati itupalẹ bioinformatic ti ilọsiwaju ti nmu awọn ibi-afẹde iwadii kan pato ṣẹ.Ifitonileti pipe diẹ sii ti jiini kokoro arun n fun ni agbara ṣiṣafihan ti awọn ilana ipilẹ ti o wa labẹ awọn ilana ti ibi wọn, eyiti o tun le pese itọkasi ti o niyelori fun awọn iwadii jinomiki ni awọn eya eukaryotic ti o ga julọ.

Syeed:Nanopore PromethION P48 + Illumina NovaSeq 6000

PacBio Sequel II


Awọn alaye Iṣẹ

Awọn abajade Ririnkiri

Awọn anfani Iṣẹ

ØỌpọ ilana ilana ilana ti o wa fun awọn ibi-afẹde iwadii oriṣiriṣi

ØAwọn kokoro arun pipe jiini pẹlu 0 Gap ẹri.

ØNi iriri giga ni apejọ jiini kokoro arun pẹlu diẹ sii ju 10,000 ti o pejọ ti o pejọ.

ØỌjọgbọn lẹhin-tita atilẹyin imọ-ẹrọ egbe nmu awọn ibeere iwadii pato diẹ sii.

Awọn pato Iṣẹ

TiteleIlana

Ile-ikawe

Didara ẹri

Ifoju-yika akoko

Nanopore 100X + Illumina 50X

Nanopore 20K

PE150

0 Aafo

30 Ọjọ

PacBio HiFi 30X

PacBio 10K

Awọn itupalẹ bioinformatics

üAise data didara iṣakoso

üApejọ Genome

üItupalẹ ẹya ara genome

üJiini iṣẹ asọye

üIṣayẹwo genomic afiwera

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Awọn ibeere Ayẹwo ati Ifijiṣẹ

Awọn ibeere Apeere:

FunDNA ayokuro:

Apeere Iru

Iye

Ifojusi

Mimo

DNA ayokuro

3 μg

20ng/μl

OD260/280 = 1.6-2.5

Fun awọn ayẹwo iṣan:

Iru apẹẹrẹ Itọju ayẹwo ti a ṣe iṣeduro Ayẹwo ipamọ ati sowo
Awọn kokoro arun Ṣe akiyesi awọn kokoro arun labẹ maikirosikopu ki o gba awọn kokoro arun ni ipele ipari wọn

Gbigbe aṣa kokoro arun (ti o ni isunmọ. 3-4.5e9 awọn sẹẹli) sinu eppendorf 1.5 tabi 2 milimita.(Tẹ lori yinyin)

Centrifuge tube naa fun iṣẹju 1 ni 14000 g lati gba awọn kokoro arun ati yọọ supernatant ni pẹkipẹki.

Pa tube naa ki o di awọn kokoro arun sinu nitrogen olomi fun o kere 30 min.Tọju tube ni -80 ℃ firiji.

Di awọn ayẹwo naa sinu omi nitrogen fun awọn wakati 3-4 ati fipamọ sinu nitrogen olomi tabi iwọn -80 si ifiṣura igba pipẹ.Ayẹwo gbigbe pẹlu yinyin gbigbẹ ni a nilo.

Sisan Iṣẹ Iṣẹ

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Ifijiṣẹ apẹẹrẹ

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Ikole ìkàwé

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Titele

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Ayẹwo data

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Lẹhin-tita awọn iṣẹ


  • Ti tẹlẹ:
  • Itele:

  • 1.Circos ti kokoro arun genome

    3

    2.Coding pupọ asọtẹlẹ

    4

    3.Comparative genomics onínọmbà: phylogenetic igi

    5

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