DNA/RNA Sequence – Illumina Sequencer
Awọn ẹya ara ẹrọ Awọn alaye Iṣẹ
| Platform | Ipo Atẹle | Awọn ohun elo |
| Illumina Nova eto | SE50 PE50 PE150 PE250 | 1. Yiyara ọkọọkan gbogbo genomes 2. Sun-un si awọn agbegbe ibi-afẹde ọkọọkan 3. Lo ilana RNA (RNA-Seq) lati ṣawari awọn iyatọ RNA aramada ati awọn aaye splice, tabi ṣe iwọn awọn mRNA fun itupalẹ ikosile pupọ. 4. Ṣe itupalẹ awọn ifosiwewe epigenetic gẹgẹbi jiini-jakejado DNA methylation ati awọn ibaraẹnisọrọ DNA-amuaradagba 5. Awọn ayẹwo alakan lẹsẹsẹ lati ṣe iwadi awọn iyatọ somatic toje, awọn subclones tumo, ati diẹ sii Iwadi oniruuru makirobia ninu eniyan tabi ni agbegbe |
Awọn anfani Iṣẹ
● Iriri ti o gbooro lori Syeed ilana ilana Illumina pẹlu ẹgbẹẹgbẹrun awọn iṣẹ akanṣe pipade pẹlu awọn oriṣi oriṣiriṣi.
● Ogbo ilana ilana atẹle pẹlu akoko kukuru-yika.
● Pari awọn iṣẹ itupalẹ bioinformatics ni isalẹ.
● Eto iṣakoso didara ti o muna: boṣewa QC ti o muna ti data atẹle.
Awọn ibeere Apeere*
| Syeed titele & amupu; Ilana titele | Iye data |
Ifojusi
| Iwọn didun | Iye | abajade QC |
| Eto Novaseq, so pọ-opin 150bp | X≤30Gb | ≥1ng/μl | ≥20 μl | ≥20ng | Ti o peye |
| 30Gb <X≤100Gb | ≥1ng/μl | ≥25 μl | ≥30ng | ||
| X>100Gb | ≥2ng/μl | ≥25 μl | ≥50ng |
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Ni afikun si ifọkansi ati iye lapapọ, apẹrẹ tente oke ti o yẹ tun nilo.
Ọ̀nà Kan ṣoṣo (Imọlẹ)
| Platform
|
Ifojusi
| Iwọn didun | abajade QC |
| HiseqXten | ≥2.5nM | ≥20μL | Ti o peye |
| NovaSeq 6000 S4 | ≥2nM | ≥30μL | |
| NovaSeg 6000 SP | ≥2nM | ≥30μL |
Jọwọ kan si wa ti awọn ayẹwo rẹ ko ba pade awọn ibeere ohun elo ibẹrẹ.
Ṣiṣẹ Iṣẹ Iṣẹ
Iṣakoso didara ìkàwé
Titele
Iṣakoso didara data
Ifijiṣẹ ise agbese
Ayẹwo Didara Data ti Ayẹwo RNA
Table 1. Statistics on data lesese.
| Apeere ID | BMKID | Aise kika | Data aise (bp) | Awọn kika mimọ (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22.870.120 | 6.861.036.000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14.717.867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Ṣe nọmba 1. Didara pinpin pẹlu kika ni ayẹwo kọọkan
olusin 2. Ipilẹ akoonu pinpin
Ṣe nọmba 3. Pipin awọn akoonu kika ni data tito lẹsẹsẹ
