DNA/RNA Sequence – Nanopore Sequencer
Awọn ẹya ara ẹrọ Awọn alaye Iṣẹ
| Platform | Iwon ikawe | Ikore data imọ-jinlẹ (Ni ẹẹẹkeji) | Nikan-mimọ Yiye | Awọn ohun elo |
| Nanopore | 8Kb, 10kb, 20kb, Ultralong, cDNA-PCR | 70-90Gb/ẹyin | 85-92% | Ipe SV, De novo, Atẹle ipari-kikun, Iso-Seq, Atọka Gene, Iwari ti methylation DNA |
Awọn anfani Iṣẹ
● Lori 5 years 'iriri lori PacBio sequencing Syeed pẹlu egbegberun ti titi ise agbese pẹlu orisirisi eya.
● BMKGENE jẹ alabaṣepọ osise ti Oxford Nanopore, pẹlu iwe-ẹri RNA/DNA meji.
● Awọn awoṣe ojulowo ti awọn olutẹẹrẹ wa pẹlu ohun elo pipe ati igbejade ilana ti o to.
● Ni ipilẹ lori pẹpẹ Nanopore, diẹ sii ju awọn iwadii ẹranko ati ọgbin Denovo 10 ti a ti tẹjade ninu awọn iwe iroyin olokiki agbaye.
Awọn ibeere apẹẹrẹ
| Apeere Iru | Iye | Ifojusi (Qubit ®) | Iwọn didun | Mimo | Awọn miiran |
| DNA jinomiki | Da lori Data ibeere | ≥20ng/μl | ≥15μl | OD260/280 = 1.7-2.2; OD260/230≥1.5; Ko tente oke ni 260 nm, ko si awọn akoran | Ifojusi nilo lati ṣe iwọn nipasẹ Qubit ati Qubit/Nanopore ≤ 2 |
| Apapọ RNA | ≥1.2μg | ≥100μg/μl | ≥15μl | OD260 / 280 = 1.7-2.5; OD260/230 = 0.5-2.5; ko si awọn ibajẹ | RIN iye ≥7.5 |
Ṣiṣẹ Iṣẹ Iṣẹ
Apeere igbaradi
Ikole ìkàwé
Titele
Itupalẹ data
Ifijiṣẹ ise agbese
Ayẹwo Didara Data ti Ayẹwo DNA
Table 1. Statistics on mọ data.
| BMKID | rawSeqNum | rawSumBase | cleanSeqNum | cleanSumBase | cleanN50Len | cleanN90Len | cleanMeanLen | cleanMaxLen | cleanMeanQual |
| DNA_BMK01 | 1.218.239 | 26.37 | 1.121.736 | 25.90 | 28.014 | 15.764 | 23.090 | 143.181 | 9 |
Ayẹwo Didara Data ti Ayẹwo RNA
Table 1. Statistics on mọ data.
| Orukọ faili | ClientID | ReadNum | BaseNum | N50 | Itumo Gigun | O pọju Gigun | MeanQscore |
| RNA_BMK001 | C2 | 8,947,708 | 4.047,230,083 | 398 | 452 | 129.227 | Q12 |
olusin 1. Ka ipari pinpin
olusin 2. Didara Dimegilio pinpin ti mọ data
Ṣe nọmba 3. Gigun ati pinpin didara didara ti data mimọ
