DNA / RNECencing - nanopore dequecenter
Awọn ẹya Awọn alaye Iṣẹ
| Pẹpẹ | Iwọn ikawe | Awọn idii data imọ-ọrọ (fun sẹẹli) | Deede-ipilẹ deede | Awọn ohun elo |
| Nanotore | 8kb, 10KB, 20kb, ultralong, cdna-pcr | 70-90GB / BEL | 85-92% | NV Pipe, De Novo, Agbara ipari kikun, ISO-SEQ, Gene> Ijinlẹ, Iwari DNA methyysations |
Awọn anfani Iṣẹ
O ju iriri ọdun 5 lori pacbio laret Syeed pẹlu ẹgbẹẹgbẹrun awọn iṣẹ pipade pẹlu ọpọlọpọ awọn eya.
BMKGEN jẹ alabaṣepọ osise ti oxford nanotore, pẹlu meji meji Syeed Rna / DNA ijẹrisi.
● Nibẹ ni awọn awoṣe akọkọ ti awọn lepa pẹlu ẹrọ pipe ati ifaworanhan to.
● Da lori pẹpẹ nanopore, diẹ sii ju ẹranko 10 ati awọn iwadii ara na ṣe kede awọn iwadii ni agbegbe awọn iwe iroyin kariaye.
Awọn ibeere ayẹwo
| Iru ayẹwo | Iye | Ifọkansi (Quitt ®) | Iwọn didun | Awọn mimọ | Awọn miiran |
| Lionomic dna | Dale lori ibeere data | ≥20ng / μl | ≥15μL | OD260 / 280 = 1.7-2; OD260 / 2302.5; Idaraya kuro ni 260 NM, ko si awọn idi pataki | Fojusi nilo lati ṣe iwọn nipasẹ Quit ati dèt / nanopore ≤ 2 |
| Lapapọ rna | ≥1.2μg | ≥100μg / μl | ≥15μL | OD260 / 280 = 1.7-2.5; Od260 / 230 = 0,5-2.5; ko si awọn idisẹ | Rin iye ≥7.5 |
Iṣẹ Iṣẹ Iṣẹ
Iyokuro apẹẹrẹ
Ile-ikawe Ile-ikawe
Iṣẹ ṣiṣe
Itupalẹ data
Ifiranṣẹ iṣẹ
Iyẹwo Didara didara data ti awọn apẹẹrẹ DNA
Tabili 1. Awọn iṣiro lori data mimọ.
| Bmkid | Rawseqnum | rawsubuse | Mimọ | mọ | nu ara | wẹ | di mimọ | numpmaxlen | alaiṣootọ |
| DNA_BMK01 | 1,218,239 | 26.37 | 1,121,736 | 25.90 | 28,014 | 15,764 | 23,090 | 143,181 | 9 |
Iyẹwo didara didara data ti apẹẹrẹ RNE
Tabili 1. Awọn iṣiro lori data mimọ.
| Orukọ faili | Alabara | Kika iwe | Baseum | No50 | Itumo | Maxlering | Itumo |
| Rna_bmk001 | C2 | 8,947,708 | 4,047,230,083 | 398 | 452 | 129,227 | Aami |
Nọmba 1. Ka pinpin gigun
Nọmba 2. Dimegiliti Dimegilio ti data mimọ
Nọmba 3. Gigun ati pinpin didara ti data mimọ
