Olu Genome
Awọn anfani Iṣẹ
● Awọn ilana ilana ilana pupọ ti o wa fun awọn ibi-afẹde iwadii oriṣiriṣi
● Ni iriri giga ni apejọ genome fungi pẹlu diẹ sii ju 10,000 pene-pipe ti o pejọ.
● Ọjọgbọn lẹhin-tita atilẹyin imọ-ẹrọ egbe nmu awọn ibeere iwadi pato diẹ sii.
Awọn pato Iṣẹ
| Iṣẹ | Ilana titele | Didara ẹri | Ifoju-yika akoko |
| Olu itanran map | Illumina 50X + Nanopore 100X | Tesiwaju N50≥2 Mb | 35 ṣiṣẹ ọjọ |
| PacBio HiFi 30X | |||
| Olu pene-pipe map | Illumina 50X+ Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X | Ipin idaduro chromosome>90% | 45 ṣiṣẹ ọjọ |
Awọn itupalẹ bioinformatics
● Aise data didara iṣakoso
● Apejọ Genome
● Ayẹwo paati Jinom
● Atọka iṣẹ Jiini
● Ayẹwo genomic afiwera
Awọn ibeere Ayẹwo ati Ifijiṣẹ
Awọn ibeere Apeere:
FunDNA ayokuro:
| Apeere Iru | Iye | Ifojusi | Mimo |
| DNA ayokuro | 1.2 μg | 20ng/μl | OD260/280 = 1.6-2.5 |
Fun awọn ayẹwo iṣan:
| Iru apẹẹrẹ | Itọju ayẹwo ti a ṣe iṣeduro | Ayẹwo ipamọ ati sowo |
| Fungus Unicellular | Ṣe akiyesi iwukara labẹ maikirosikopu ki o gba wọn ni ipele alapin wọn Gbigbe aṣa naa (ti o ni isunmọ. 3-4.5e9 awọn sẹẹli) sinu eppendorf 1.5 tabi 2 milimita.(Tẹ lori yinyin) Centrifuge tube fun iṣẹju 1 ni 14000 g lati gba awọn kokoro arun ati yọọ supernatant ni pẹkipẹki. Pa tube naa ki o di awọn kokoro arun sinu nitrogen olomi fun o kere ju wakati 1-3.Tọju tube ni -80 ℃ firiji. | Di awọn ayẹwo naa sinu omi nitrogen fun awọn wakati 3-4 ati fipamọ sinu nitrogen olomi tabi iwọn -80 si ifiṣura igba pipẹ.Ayẹwo gbigbe pẹlu yinyin gbigbẹ ni a nilo. |
| Makiro Fungus | Tissue ni ipele ti n dagba ni itara ni a ṣe iṣeduro. Fi omi ṣan pẹlu omi ti ko ni endotoxin, lẹhinna 70% ethonal. Fi ayẹwo pamọ sinu awọn tubes cryo. |
Sisan Iṣẹ Iṣẹ
Ifijiṣẹ apẹẹrẹ
Ikole ìkàwé
Titele
Itupalẹ data
Lẹhin-tita awọn iṣẹ
1.Circos aworan atọka lori olu genomic irinše
2.Comparative genomics onínọmbà: Phylogenetic igi
