Long Non-ifaminsi Sequencing-Illumina
Awọn anfani Iṣẹ
●Ayẹwo apapọ ti mRNA ati lncRNA: nipa pipọ pipọ ti awọn iwe afọwọkọ mRNA pẹlu iwadi ti lncRNA ati awọn ibi-afẹde wọn, o ṣee ṣe lati ni iwoye-jinlẹ ti ilana ilana ti o wa labẹ esi cellular.
●Amoye nla: Ẹgbẹ wa n mu iriri lọpọlọpọ wa si gbogbo iṣẹ akanṣe, pẹlu igbasilẹ orin kan ti sisẹ lori awọn apẹẹrẹ 23,000 ni BMK ti o ni awọn iru apẹẹrẹ oniruuru ati awọn iṣẹ akanṣe lncRNA.
●Iṣakoso Didara lile: A ṣe awọn aaye iṣakoso mojuto ni gbogbo awọn ipele, lati apẹẹrẹ ati igbaradi ile-ikawe si ilana ati bioinformatics. Abojuto to ṣe pataki yii ṣe idaniloju ifijiṣẹ ti awọn abajade didara to gaju nigbagbogbo.
●Post-Tita Support: Ifaramo wa ti kọja ipari iṣẹ akanṣe pẹlu akoko iṣẹ-iṣẹ 3-osu lẹhin-tita. Lakoko yii, a funni ni atẹle iṣẹ akanṣe, iranlọwọ laasigbotitusita, ati awọn akoko Q&A lati koju eyikeyi awọn ibeere ti o ni ibatan si awọn abajade.
Awọn ibeere Ayẹwo ati Ifijiṣẹ
| Ile-ikawe | Platform | Data ti a ṣe iṣeduro | Data QC |
| rRNA depleted ikawe itọnisọna | Imọlẹ PE150 | 10-16 Gb | Q30≥85% |
Nucleotides:
| Konc.(ng/μl) | Iye (μg) | Mimo | Òtítọ́ |
| ≥ 80 | ≥ 0.8 | OD260/280 = 1.7-2.5 OD260/230 = 0.5-2.5 Lopin tabi ko si amuaradagba tabi idoti DNA ti o han lori jeli. | RIN≥6.0; 5.0≥28S/18S≥1.0; ni opin tabi ko si igbega ipilẹ |
● Awọn ohun ọgbin:
Gbongbo, Jeyo tabi Petal: 450 mg
Ewe tabi Irugbin: 300 mg
Eso: 1.2 g
● Ẹranko:
Okan tabi ifun: 450 mg
Viscera tabi Ọpọlọ: 240 mg
Isan: 600 mg
Egungun, Irun tabi Awọ: 1.5g
● Arthropods:
Kokoro: 9g
Crustacea: 450 mg
● Odidi ẹjẹ:2 tubes
● Awọn sẹẹli: 106 awọn sẹẹli
● Omi ara ati Plasma: 6ml
Niyanju Ifijiṣẹ Apeere
Apoti: tube centifuge 2 milimita (a ko ṣeduro bankanje Tin)
Ifamisi apẹẹrẹ: Ẹgbẹ+ ṣe ẹda fun apẹẹrẹ A1, A2, A3; B1, B2, B3.
Gbigbe:
1. Gbẹ-yinyin: Awọn ayẹwo nilo lati wa ni aba ti awọn apo ati ki o sin ni gbẹ-yinyin.
2. Awọn tubes RNAstable: Awọn ayẹwo RNA le gbẹ ni tube imuduro RNA (fun apẹẹrẹ RNAstable®) ati gbigbe ni iwọn otutu yara.
Sisan Iṣẹ Iṣẹ
Apẹrẹ adanwo
Ifijiṣẹ apẹẹrẹ
RNA isediwon
Ikole ìkàwé
Titele
Itupalẹ data
Lẹhin-tita awọn iṣẹ
Bioinformatics
Iyatọ Gene Expression (DEGs) onínọmbà
Quantification ti lncRNA ikosile – iṣupọ
Imudara ti awọn jiini ibi-afẹde lncRNA
MRNA apapọ ati itupalẹ ipo lncRNA - Idite Circos (aarin aarin jẹ mRNA ati cycrlce inu jẹ lncRNA)
Ṣawari awọn ilọsiwaju ti o rọrun nipasẹ BMKGene's lncRNA equencing awọn iṣẹ nipasẹ ikojọpọ ti awọn atẹjade.
Ji, H. et al. (2020) 'Idamọ, asọtẹlẹ iṣẹ, ati ijẹrisi bọtini lncRNA ti awọn lncRNA ti o ni aapọn tutu ninu ẹdọ eku', Awọn ijabọ Imọ-jinlẹ 2020 10: 1, 10 (1), pp. 1–14. doi: 10.1038 / s41598-020-57451-7.
Jia, Z. et al. (2021) 'Onínọmbà Transcriptomic Integrative Ṣafihan Ilana Ajesara fun Igara Carp Kan ti o wọpọ CyHV-3-Resistant', Awọn iwaju ni Imunoloji, 12, p. 687151. doi: 10.3389 / FIMMU.2021.687151 / BIBTEX.
Wang, XJ ati al. (2022) 'Multi-Omics Integration-based Prioritization of Idije Endogenous RNA Regulation Networks in Small Cell Lung Cancer: Molecular Characteristics and Drug Candidates', Frontiers in Oncology, 12, p. 904865. doi: 10.3389 / FONC.2022.904865 / BIBTEX.
Xiao, L. et al. (2020) 'Ipinpin Jiini ti nẹtiwọọki coexpression pupọ ti o wa labẹ photosynthesis ni Populus', Iwe akọọlẹ Imọ-ẹrọ Ohun ọgbin, 18 (4), oju-iwe. 1015–1026. doi: 10.1111 / PBI.13270.
Zheng, H. et al. (2022) 'Nẹtiwọọki Ilana Ilana Kariaye fun Ifihan Jiini Dysregulated ati Iforukọsilẹ Metabolic Ajeji ninu Awọn sẹẹli Ajẹsara ninu Microenvironment ti Arun Graves ati Hashimoto's Thyroiditis', Awọn iwaju ni Imunoloji, 13, p. 879824. doi: 10.3389 / FIMMU.2022.879824 / BIBTEX.
