Metatranscriptome Sequencing
Awọn ẹya ara ẹrọ Iṣẹ
● idinku rRNA atẹle nipa igbaradi ile-ikawe mRNA itọsọna.
● Sequencing lori Illumina NovaSeq.
Awọn anfani Iṣẹ
●Ṣe iwadi Awọn iyipada ti Awọn agbegbe Microbial Complex:Eyi ṣẹlẹ ni ipele transcriptional ati ṣawari awọn jiini tuntun ti o pọju.
●Ṣalaye Awọn Ibaṣepọ Agbegbe Microbial pẹlu Olugbalejo tabi Ayika.
●Okeerẹ Bioinformatic Analysis: Eyi n pese awọn oye lori taxonomic agbegbe ati awọn akopọ iṣẹ, bakanna bi itupalẹ ikosile jiini iyatọ.
●Apejuwe Jiini gbooro:Lilo awọn apoti isura infomesonu iṣẹ jiini ti ode oni fun alaye ikosile jiini ti alaye ti awọn agbegbe makirobia.
●Atilẹyin Tita-lẹhin:Ifaramo wa kọja ipari iṣẹ akanṣe pẹlu oṣu mẹta lẹhin-tita akoko iṣẹ. Lakoko yii, a funni ni atẹle iṣẹ akanṣe, iranlọwọ laasigbotitusita, ati awọn akoko Q&A lati koju eyikeyi awọn ibeere ti o ni ibatan si awọn abajade.
Awọn pato Iṣẹ
| Sequencing Syeed | Ilana titele | Data niyanju | Iṣakoso Didara Data |
| Illumina NovaSeq | PE150 | 12Gb | Q30≥85% |
Awọn ibeere apẹẹrẹ
| Ifojusi (ng/µL) | Apapọ iye (µg) | Iwọn (µL) | OD260/280 | OD260/230 | RIN |
| ≥50 | ≥1.0 | ≥20 | 1.8-2.0 | 1.0-2.5 | ≥6.5 |
Sisan Iṣẹ Iṣẹ
Ifijiṣẹ apẹẹrẹ
Ikole ìkàwé
Titele
Itupalẹ data
Lẹhin-tita awọn iṣẹ
Pẹlu itupale wọnyi:
● Ṣiṣeto Iṣakoso Didara Data
● Apejọ tiransikiripiti
● Taxonomic Annotation ati Ọpọlọpọ
● Atọka Iṣẹ-ṣiṣe ati Ọpọlọpọ
● Iṣiro Iṣafihan ati Itupalẹ Iyatọ
Pinpin Taxonomic ti ayẹwo kọọkan:
Beta oniruuru onínọmbà: UPGMA
Atọka ti iṣẹ-ṣiṣe - GO lọpọlọpọ
Iyatọ taxonomy opo - LEFSE
Ṣawari awọn ilọsiwaju ti o ni irọrun nipasẹ BMKGene's meta transcriptomics sequencing awọn iṣẹ nipasẹ ikojọpọ ti awọn atẹjade.
Lu, Z. et al. (2023) 'Ifarada acid ti awọn kokoro arun lactate-lilo ti aṣẹ Bacteroidales ṣe alabapin si idena ti ruminal acidosis ninu awọn ewurẹ ti o baamu si ounjẹ ifọkansi giga’,Ounjẹ Eranko, 14, ojú ìwé 130–140. doi: 10.1016/J.ANINU.2023.05.006.
Orin, Z. et al. (2017) 'Mikrobiota ti iṣẹ ṣiṣe ṣiṣi silẹ ni bakteria-ipinle ti ibilẹ nipasẹ awọn ampili ti o ga-giga ati ilana ilana metatranscriptomics',Furontia ni Maikirobaoloji, 8 (Jul). doi: 10.3389/FMICB.2017.01294/FULL.
Wang, W. et al. (2022) 'A ṣe awari Mycoviruses aramada lati inu Iwadi Metatranscriptomics ti Fungus Alternaria Phytopathogenic’,Awọn ọlọjẹ, 14 (11), ojú ìwé. 2552. doi: 10.3390 / V14112552 / S1.
Wei, J. et al. (2022) 'Onínọmbà metatranscriptome ti o jọra ṣe afihan ibajẹ ti awọn metabolites Atẹle ọgbin nipasẹ awọn beetles ati awọn symbionts ikun wọn’,Molikula Ekoloji, 31 (15), oju-iwe 3999-4016. doi: 10.1111 / MEC.16557.
