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条形 isibhengezo-03

Imikhiqizo

I-Assay ye-Chromatin Efinyelelekayo Ye-Transposase Enokulandelanisa Okuphezulu Kokudlula (i-ATAC-seq)

I-Assay ye-Chromatin Efinyelelekayo Ye-Transposase Enokulandelanisa Okuphezulu Kokudlula (i-ATAC-seq) Isithombe Esifakiwe
  • I-Assay ye-Chromatin Efinyelelekayo Ye-Transposase Enokulandelanisa Okuphezulu Kokudlula (i-ATAC-seq)
  • I-Assay ye-Chromatin Efinyelelekayo Ye-Transposase Enokulandelanisa Okuphezulu Kokudlula (i-ATAC-seq)
  • I-Assay ye-Chromatin Efinyelelekayo Ye-Transposase Enokulandelanisa Okuphezulu Kokudlula (i-ATAC-seq)
  • I-Assay ye-Chromatin Efinyelelekayo Ye-Transposase Enokulandelanisa Okuphezulu Kokudlula (i-ATAC-seq)
  • I-Assay ye-Chromatin Efinyelelekayo Ye-Transposase Enokulandelanisa Okuphezulu Kokudlula (i-ATAC-seq)

I-ATAC-seq iyindlela yokulandelanisa ephezulu yokuhlaziya ukufinyeleleka kwe-chromatin ebanzi yofuzo, okubalulekile ekulawuleni i-epigenetic yomhlaba wonke ekukhulumeni kofuzo.Ama-adaptha alandelanayo afakwa ezifundeni ze-chromatin nge-hyperactive Tn5 transposase.Ngemuva kokukhulisa i-PCR, kwakhiwa umtapo wolwazi wokulandelanisa.Zonke izifunda ze-chromatin ezivulekile zingatholwa ngaphansi kwesimo esithile sesikhathi sesikhala, hhayi kuphela kumasayithi abophezelayo we-transcription factor, noma isifunda esithile esilungisiwe se-histone.


Imininingwane Yesevisi

I-Bioinformatics

Imiphumela yedemo

Izinzuzo Zesevisi

● Ukuhlaziya Okubanzi: Ipayipi lokuhlaziya lochwepheshe ukuze kuhlangatshezwane nezidingo ezihlukahlukene zokuhlaziya ezenziwe ngokwezifiso;

● Ulwazi Olubanzi: Amakhulu amaphrojekthi aqedwe ngempumelelo kuze kube manje, okuhlanganisa amasampula ezilwane nezitshalo.

Imininingwane Yesevisi

Ipulatifomu: Iplatifomu ye-Illumina NovaSeq

Uhlobo lwelabhulali: ATAC-seq

Okuphumayo kwedatha okunconyiwe: ≥20M ukufundwa

Izidingo Zesampula

Uhlobo lwesampula: Izicubu, amaseli aphilayo, noma amaseli afriziwe nokunye

Inombolo yeseli: ≥ 5×105amaseli, inombolo yeseli enembile iyisihluthulelo sempumelelo yocwaningo;

Isisindo sezicubu: ≥ 200mg izicubu ezintsha;

Igazi: ≥ 2 mL

Ukugeleza Komsebenzi Wesevisi

Isampula ye-QC

Idizayini yokuhlola

ukulethwa kwesampula

Ukulethwa kwesampula

Ukuhlolwa komshayeli

Ukukhishwa kwe-DNA

Ukulungiselela Umtapowolwazi

Ukwakhiwa komtapo wolwazi

Ukulandelanisa

Ukulandelanisa

Ukuhlaziywa kwedatha

Ukuhlaziywa kwedatha

Ngemva kokudayiswa Services

Amasevisi angemuva kokuthengisa

  • Okwedlule:
  • Olandelayo:

    • 表观-02

    1.I-Heatmap ku-ATAC ifunda ukusatshalaliswa ku-TSS nasezifundeni eziseduze (±3 kb)

    3 (1)2.Ukusatshalaliswa kwesifunda se-chromatin ezingxenyeni ezihlukene ze-genome

    4 (1)

    3.Umehluko omkhulu wokubiza phakathi kwamaqembu

    5 (1)

    thola isaphulelo

    Bhala umlayezo wakho lapha futhi usithumelele wona

    Umkhiqizoizigaba

    Thumela umlayezo wakho kithi:

    Chofoza u-enter ukuze useshe noma u-ESC ukuze uvale