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条形 isibhengezo-03

Imikhiqizo

Amagciwane Aphelele I-Genome

I-Biomarker Technologies ihlinzeka ngensizakalo yokulandelanisa ekwakheni i-genome ephelele yebhaktheriya enegebe eliziro.Ukuhamba komsebenzi okuyinhloko kwebhaktheriya ukwakhiwa okuphelele kofuzo kufaka phakathi ukulandelana kwesizukulwane sesithathu, ukuhlanganisa, isichasiselo esisebenzayo kanye nokuhlaziywa okuthuthukisiwe kwe-bioinformatic okufeza imigomo ethile yocwaningo.Iphrofayili ebanzi yofuzo lwebhaktheriya inika amandla ukudalulwa kwezinqubo ezibalulekile eziyisisekelo zezinqubo zabo zebhayoloji, okungase futhi kunikeze ireferensi ebalulekile yocwaningo lwe-genomic ezinhlotsheni eziphakeme ze-eukaryotic.

Inkundla:I-Nanopore PromethION P48 + Illumina NovaSeq Platform

I-PacBio Sequel II


Imininingwane Yesevisi

Imiphumela yedemo

Izinzuzo Zesevisi

● Amasu amaningi okulandelana atholakalayo ezinhlosweni ezihlukene zocwaningo

● I-genome egcwele amagciwane ene-Gap engu-0 eqinisekisiwe.

● Unolwazi oluphezulu ekuhlanganiseni kofuzo lwebhaktheriya ehlanganisa i-genome engaphezu kuka-10,000.

● Ithimba losekelo lobuchwepheshe ngemva kokuthengisa elenza izidingo ezithile zocwaningo.

Imininingwane Yesevisi

UkulandelanisaIsu

Umtapowolwazi

Ikhwalithi iqinisekisiwe

Isikhathi sokujika esilinganiselwe

I-Nanopore 100X + Illumina 50X

I-Nanopore 20K

I-PE150

0 igebe

Izinsuku ezingama-30

I-PacBio HiFi 30X

I-PacBio 10K

I-Bioinformatics ihlaziya

● Ukulawulwa kwekhwalithi yedatha eluhlaza

● Ukuhlanganiswa kwe-genome

● Ukuhlaziywa kwengxenye ye-genome

● Isichasiselo somsebenzi wofuzo

● Ukuhlaziywa kwe-genomic okuqhathanisayo

2

Izidingo Zesampula Nokulethwa

Izimfuneko zesampula:

NgobaIzingcaphuno ze-DNA:

Uhlobo lwesampula

Inani

Ukugxila

Ubumsulwa

Izingcaphuno ze-DNA

> 2 μg

> 20 ng/μl

OD260/280= 1.6-2.5

Okwamasampula ezicubu:

Uhlobo lwesampula Ukwelashwa okuyisampula okunconyiwe Isampula yokugcina nokuthunyelwa
Amagciwane Bheka amagciwane ngaphansi kwesibonakhulu futhi uqoqe amagciwane esigabeni sawo sokuchayeka

Dlulisa isiko lebhaktheriya (eliqukethe cishe amaseli angu-3-4.5e9) ku-1.5 noma 2 ml eppendorf.(Qhubekela eqhweni)

I-Centrifuge ishubhu iminithi elingu-1 ku-14000 g ukuqoqa amabhaktheriya futhi ususe i-supernatant ngokucophelela.

Vala ishubhu bese ufriza amabhaktheriya ku-nitrogen ewuketshezi okungenani imizuzu engama-30.Gcina ishubhu ku -80 ℃ esiqandisini.

Friza amasampula ku-nitrogen ewuketshezi amahora angu-3-4 futhi uwagcine ku-nitrogen ewuketshezi noma -80 degree ekubhukeni isikhathi eside.Kudingeka isampula yokuthunyelwa ngeqhwa elomile.

Ukugeleza Komsebenzi Wesevisi

ukulethwa kwesampula

Ukulethwa kwesampula

Ukulungiselela Umtapowolwazi

Ukwakhiwa komtapo wolwazi

Ukulandelanisa

Ukulandelanisa

Ukuhlaziywa kwedatha

Ukuhlaziywa kwedatha

Ngemva kokudayiswa Services

Amasevisi angemuva kokuthengisa


  • Okwedlule:
  • Olandelayo:

  • 1.Ama-Circos of bacteria genome

    3

    2.Ukubikezela kofuzo lwekhodi

    4

    Ukuhlaziywa kwe-3.Comparative genomics: isihlahla se-phylogenetic

    5

    thola isaphulelo

    Bhala umlayezo wakho lapha futhi usithumelele wona

    Thumela umlayezo wakho kithi: