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I-BMKMANU S1000 Spatial Transcriptome

Isithombe Esifakiwe se-BMKMANU S1000 Spatial Transcriptome
  • I-BMKMANU S1000 Spatial Transcriptome
  • I-BMKMANU S1000 Spatial Transcriptome

Ukuhleleka kwendawo kwamaseli kudlala indima ebalulekile ezinqubweni ezihlukahlukene zebhayoloji, njengokungenwa kwamasosha omzimba, ukuthuthukiswa kombungu, njll. Ukulandelana kwe-Spatial transcriptome, okubonisa iphrofayela yesisho sofuzo kuyilapho kugcinwa ulwazi lwendawo yendawo, kunikeze imininingwane emihle ekwakhiweni kwezicubu zezinga le-transcriptome.Ngokuthuthuka kobuchwepheshe, i-ultra-clear tissue morphology kanye nomehluko wangempela wesakhiwo sokuchazwa kwamangqamuzana wendawo kudingeka kufundwe ngokulungiswa okuphezulu.I-BMKGENE inikeza insizakalo yokulandelana kwe-spatial transcriptome ephelele, yokuma eyodwa ukusuka kumasampula ukuya emininingwaneni yebhayoloji.

Ubuchwepheshe be-Spatial transcriptomics bunike amandla imibono yamanoveli enkundleni yocwaningo eyahlukahlukene ngokuxazulula iphrofayili yesisho sofuzo ngokuqukethwe kwendawo kumasampula ahlukahlukene.

I-Spatial transcriptome chip: BMKMANU S1000

Ipulatifomu: I-Illumina NovaSeq Platform


Imininingwane Yesevisi

I-Bioinformatics

Imiphumela yedemo

I-BMKMANU S1000 Spatial Transcriptome Technical Scheme

S1000.

Izinzuzo ze-BMKMANU S1000

1) Ukulungiswa kwamaselula angaphansi: Indawo ngayinye yokuthwebula iqukethe > Amabala Abhakhodi Ayizigidi ezingu-2 anobubanzi obungu-2.5 µm kanye nesikhala esingu-5 µm phakathi kwezindawo zamabala, okuvumela ukuhlaziywa kwe-spatical transcriptome ngokulungiswa kwamaselula amancane (5 µm).

I-s1000 (1)

I-2) Ukuhlaziywa kwesinqumo samazinga amaningi: Ukuhlaziya okuguquguqukayo kwamazinga amaningi kusukela ku-100 μm kuya ku-5 μm ukuze kuxazululwe izici zezicubu ezihlukahlukene ngokulungiswa okuphelele.

I-s1000 (2)

3) Iphrofayili ye-transcriptome ebanzi: Imibhalo ethathwe kuso sonke isilayidi sethishu ingahlaziywa, ngaphandle kokuvinjelwa enanini lezakhi zofuzo eziqondiwe kanye nendawo okuqondiwe kuyo.

I-s1000 (3)

Imininingwane Yesevisi

Umtapowolwazi

Isu lokulandelanisa

Okuphumayo kwedatha kuyanconywa

Ilabhulali ye-cDNA ye-S1000

I-BMKMANU S1000-Illumina PE150

60Gb/isampula

Izidingo Zesampula

Isampula

Inombolo

Usayizi

Ikhwalithi ye-RNA

Ibhulokhi yethishu eshumekiwe ye-OCT

2-3 amabhlogo/ isampula

Cishe.6.8x6.8x6.8 mm3

I-RIN≥7

Ukuze uthole imininingwane eyengeziwe ngomhlahlandlela wokulungiselela isampula kanye nokuhamba komsebenzi wesevisi, sicela ukhululeke ukukhuluma no-a

Ukugeleza Komsebenzi Wesevisi

Isampula ye-QC

Idizayini yokuhlola

ukulethwa kwesampula

Ukulethwa kwesampula

Ukuhlolwa komshayeli

Ukuhlola kwangaphambili

Ibhakhodi yendawo

Ibhakhodi yendawo

Ukulungiselela Umtapowolwazi

Ukwakhiwa komtapo wolwazi

Ukulandelanisa

Ukulandelanisa

Ukuhlaziywa kwedatha

Ukuhlaziywa kwedatha

Ngemva kokudayiswa Services

Amasevisi angemuva kokuthengisa

  • Okwedlule:
  • Olandelayo:

    • 10x (9)

    Idatha ekhiqizwe yi-BMKMANU S1000 ihlaziywa kusetshenziswa isoftware ethi “BSTMatrix”, eklanywe ngokuzimela yi-BMKGENE, okuhlanganisa:

    1) Ukukhiqizwa kwe-matrix ye-Gene expression
    2) HE image processing
    3) Ihambisana nesoftware yenkampani yangaphandle ukuze ihlaziywe
    4) I-"BSTViewer" eku-inthanethi isiza ukuthola imiphumela yokubona ngezinqumo ezihlukene.

    1.Spot clustering

    I-s1000 (4)

    2.Ukusatshalaliswa kwendawo

    I-s1000 (5)I-s1000 (6)

    Nothi: Isinqumoizinga=13 (100 µm, kwesobunxele); 7 (50 µm, kwesokudla)

    3.Imephu yokushisa ehlanganisayo ye-marker expression

    I-s1000 (7)

    4.I-Inter-sample data analysis

    I-s1000 (8)

     

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