I-Illumina ne-BGI
Izici
●Izinkundla:I-Illumina NovaSeq 6000, NovaSeq , HiSeq X Ten kanye ne-BGI-DNB-T7
●Amamodi wokulandelanisa:PE50, PE100, PE150, PE250
●Ukulawulwa kwekhwalithi yemitapo yolwazi ngaphambi kokulandelana
●Ilandelanisa ukulethwa kwedatha ne-QC:ukulethwa kombiko we-QC kanye nedatha eluhlaza ngefomethi ye-fastq ngemuva kokufundwa kwe-demultiplexing nokuhlunga i-Q30.
Izinzuzo Zesevisi
●Ukuhlukahluka kwezinsizakalo zokulandelanisa:ikhasimende lingakhetha ukulandelana ngomzila, iseli eligelezayo noma inani ledatha.
●Ukuhlukahluka kwezinkundla:Imitapo yolwazi ye-DNB ingadluliselwa kumapulatifomu e-Illumina
●Umuzwa obanzi kuplathifomu yokulandelana kwe-Illumina:ngezinkulungwane zamaphrojekthi avaliwe anezinhlobo ezahlukahlukene.
●Ukulethwa kokulandelanisa umbiko we-QC:ngamamethrikhi ekhwalithi, ukunemba kwedatha kanye nokusebenza kukonke kwephrojekthi yokulandelanisa.
●Inqubo yokulandelanisa yabantu abadala:ngokuhamba kwesikhathi esifushane.
●Ukulawulwa Kwekhwalithi Okuqinile: sisebenzisa izimfuneko eziqinile ze-QC ukuze siqinisekise ukulethwa kwemiphumela ehlala njalo yekhwalithi ephezulu.
Izimfuneko zesampula*
Ukulandelana Kwendlela Eyingxenye
| Inani ledatha (X) | I-Concentration (qPCR/nM) | Ivolumu |
| X ≤ 50 Gb | ≥ 2 nM | ≥ 20 μl |
| 50 Gb ≤ X <100 Gb | ≥ 3 nM | ≥ 20 μl |
| X ≥ 100 Gb | ≥ 4 nM | ≥ 20 μl |
Umzila Owodwa (Illumina)
| Inkundla | I-Concentration (qPCR/nM) | Ivolumu |
| I-HiSeq X Ten | ≥ 2 nM | ≥ 20 μl |
| I-NovaSeq 6000 SP | ≥ 1 nM | ≥ 25 μl |
| I-NovaSeq 6000 S4 | ≥ 1.5 nM | ≥ 25 μl |
| I-NovaSeq X | ≥ 1.5 nM | ≥ 25 μl |
| I-BGI-DNBSEQ-T7 | ≥ 1.5 nM | ≥ 25 μl |
Ngaphezu kokugxilisa ingqondo nenani eliphelele, iphethini yokuphakama efanele nayo iyadingeka.
Sicela usithinte uma amasampula akho engahlangabezani nezidingo zento yokuqala.
Uhlelo lokusebenza lwesevisi
Ukulawulwa kwekhwalithi yelabhulali
Ukulandelanisa
Ukulawulwa kwekhwalithi yedatha
Ukulethwa kwephrojekthi
Umbiko we-Library QC
Umbiko wekhwalithi yomtapo wolwazi unikezwa ngaphambi kokulandelana, ukuhlola inani lomtapo wolwazi, nokuhlukaniswa.
Ilandelanisa umbiko we-QC
Ithebula 1. Izibalo zokulandelana kwedatha.
| I-ID yesampula | I-BMKID | Okuluhlaza kufundeka | Idatha eluhlaza (bp) | Ukufundwa okuhlanzekile (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Umfanekiso 1. Ukusatshalaliswa kwekhwalithi ngokuhambisana nokufundwa kwesampula ngayinye
Umfanekiso 2. Isisekelo sokusatshalaliswa kokuqukethwe
Umfanekiso 3. Ukusatshalaliswa kokuqukethwe okufundiwe ekulandeleni idatha
