Imikhiqizo

I-DNA / RNA Sequencing - I-Nanopore Sequencer

Ukulandelana kwe-ONT Molecule kuyinto eyodwa ye-molecule real-time kagesi ngesikhathi sobuchwepheshe obususelwa kuma-nanopores, umgomo wokulandelana wepulatifomu ngamunye uyefana. I-DNA / RNA ekhuphuke kabili izobopha amaprotheni ama-nanoporous eshumeke e-biofilm futhi igweme ngaphansi kokuhola kwamaprotheni kagesi, ngaphansi kwesenzo sozinhlaka ze-biofilm, i-DNA / RNADSS STRANDS idlula ku-nanopore Channel Protein ngokuqinisekile isilinganiso. Ngenxa yokwehluka kwezinto zamakhemikhali zezisekelo ezahlukahlukene ku-DNA / RNA STRAND, lapho i-molecule eyodwa noma i-DNA molecule idlula esiteshini se-nanopore, kuzodala ukushintshwa kwezimpawu ezihlukile zikagesi. Ngokuthola nokuhambelana nalezi zimpawu, izinhlobo ezihambisanayo zezisekelo zingabalwa, futhi ukutholwa kwangempela kokulandelana kungaphothulwa.

Xhumana nathi

Imininingwane ye-Service

Umphumela wedemo

Imininingwane Yesevisi Izici

Indawo yokuqhudelana	Ubukhulu bekholabhulali	Isivuno sedatha ye-Theoretical (iseli ngalinye)	Ukunemba kwesisekelo esisodwa	Izinja
I-nanopore	I-8KB, 10KB, 20KB, Ultralong, CDNA-PCR	I-70-90GB / iseli	85-92%	I-SV Calling, de Novo, Ukulandelana Okugcwele Okugcwele, I-ISO-SEQ, Isichasiselo seGene, Ukutholwa Kwe-DNA methylation

Izinzuzo zensizakalo

● Isikhathi esingaphezu kweminyaka engu-5 ngokuhlangenwe nakho kweminyaka engu-5 kwiplatifomu yokulandelana kwePacbio ngezinkulungwane zamaphrojekthi avaliwe ngezinhlobo ezahlukahlukene.
● I-BMKGENE ingumlingani osemthethweni we-Oxford Nanopore, enesitifiketi se-RNA / DNA Certification.
● Kunamamodeli ajwayelekile we-sequoncers anemishini ephelele nokulandelana ngokwanele kokufakelwa.
● Kususelwa epulatifomu ye-nanopore, izilwane ezingaphezu kwezingu-10 ezicwaningayo zesilwane kanye nezitshalo ze-denovo zishicilelwe kumaphephabhuku adume kakhulu emhlabeni jikelele.

Izidingo zesampula

Uhlobo lwesampula

Inani

I-Conf Consection (Qubit ®)

Ukuvimbanisa

Ubumsulwa

Abanye

I-genomic DNA

Kuya ngesidingo sedatha

≥20ng / μl

≥15μL

I-OD260 / 280 = 1.7-2.2;

I-OD260 / 2300≥1.5;

Sula inani eliphakeme ngo-260 nm, azikho izinto zokungcola

Ukuhlushwa kudinga ukukalwa nguQubit noQubit / Nanopore ≤ 2

Ingqikithi ye-RNA

≥1.2μg

≥100μg / μl

≥15μL

I-OD260 / 280 = 1.7-2.5;

I-OD260 / 230 = 0.5-2.5; Azikho izinto

I-Rin Face ≥7.5

Ukuhamba komsebenzi wesevisi

Ukulungiswa kwesampula

Ukwakhiwa Kwelabhulali

Ukulandelana

Ukuhlaziywa kwedatha

Ukulethwa Kwephrojekthi

Okwedlule: I-Illumina eyenziwe imitapo yolwazi

Olandelayo: Ukulandelana Okucacisiwe Kwe-Locus Amplified Coverment (SLAF-SEQ)

Ukuhlolwa kwekhwalithi yedatha yesampula ye-DNA

Ithebula 1. Izibalo ngemininingwane ehlanzekile.

IBMKID	rawseqnum	i-rawsumbase	hlanza	indawo edliwayo	I-Cleann500len	I-CleanN90len	I-CleanmeAnlen	I-Cleanmaxlen	indawo yokuhlanzaka
I-DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9