Imikhiqizo

Ukulandelana kwe-DNA/RNA - I-Nanopore Sequencer

Ukulandelana kwe-DNA/RNA - Isithombe Esifakiwe Se-Nanopore Sequencer

Ukulandelana kwe-ONT kuyi-molecule eyodwa yobuchwepheshe bokulandelanisa isignali kagesi yesikhathi sangempela ngokusekelwe kuma-nanopores, isimiso sokulandelana seplathifomu ngayinye siyefana.I-DNA/RNA enemicu emibili izobophezela kuphrotheni e-nanoporous eshumekwe ku-biofilm futhi ivuleke ngaphansi kokuhola kwe-motor protein, ngaphansi kwesenzo somehluko kagesi ezinhlangothini zombili ze-biofilm, imicu ye-DNA/RNA idlula kuphrotheni yesiteshi se-nanopore endaweni ethile. isilinganiso.Ngenxa yomehluko wezakhiwo zamakhemikhali ezisekelweni ezihlukene ku-DNA/RNA strand, lapho isisekelo esisodwa noma i-molecule ye-DNA idlula kumzila we-nanopore, izobangela ukushintshwa kwamasignali ahlukene kagesi.Ngokuthola nokuhambisana nalezi zimpawu, izinhlobo zesisekelo ezihambisanayo zingabalwa, futhi ukutholwa kwesikhathi sangempela sokulandelana kungaqedwa.

Xhumana nathi

Imininingwane Yesevisi

Umphumela wedemo

Imininingwane Yesevisi Izici

Inkundla	Usayizi Wezincwadi	I-Theory Data Yield (Ngeseli)	Ukunemba kwesisekelo esisodwa	Izinhlelo zokusebenza
I-Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Iseli	85-92%	Ukushaya kwe-SV, i-De novo, ukulandelana kobude obugcwele, i-Iso-Seq, isichasiselo seGene, Ukutholwa kwe-DNA methylation

Izinzuzo Zesevisi

● Umuzwa ongaphezu kweminyaka emi-5 kuplathifomu yokulandelanisa ye-PacBio enezinkulungwane zamaphrojekthi avaliwe anezinhlobo ezihlukahlukene.
● I-BMKGENE inguzakwethu osemthethweni we-Oxford Nanopore, onenkundla ekabili yesitifiketi se-RNA/DNA.
● Kunamamodeli ajwayelekile abalandelanayo abanamathuluzi aphelele kanye nokuphuma kokulandelana okwanele.
● Ngokusekelwe kuplathifomu ye-Nanopore, ucwaningo lwe-Denovo lwezilwane nezitshalo olungaphezu kuka-10 lushicilelwe kumajenali adumile emhlabeni jikelele.

Izidingo Zesampula

Uhlobo lwesampula

Inani

I-Concentrtion(Qubit ®)

Ivolumu

Ubumsulwa

Abanye

I-Genomic DNA

Kuya Ngesidingo Sedatha

≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260/230≥1.5;

Sula inani eliphakeme ku-260 nm, akukho ukungcola

Ukugxila kudinga ukulinganiswa nge-Qubit ne-Qubit/Nanopore ≤ 2

Isamba se-RNA

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5; akukho ukungcola

I-RIN inani ≥7.5

Uhlelo lokusebenza lwesevisi

Ukulungiselela isampula

Ukwakhiwa komtapo wolwazi

Ukulandelanisa

Ukuhlaziywa kwedatha

Ukulethwa kwephrojekthi

Okwedlule: Ukulandelana kwe-DNA/RNA - I-Illumina Sequencer

Olandelayo: I-Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

Ukuhlolwa Kwekhwalithi Yedatha Yesampula Ye-DNA

Ithebula 1. Izibalo zedatha ehlanzekile.

I-BMKID	rawSeqNum	rawSumBase	cleanSeqNum	cleanSumBase	I-cleanN50Len	i-cleanN90Len	cleanMeanLen	cleanMaxLen	cleanMeanQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9