Imikhiqizo

Amalabhulali enziwe kusengaphambili e-DNBSEQ

I-DNBSEQ, eyathuthukiswa yi-MGI, ubuchwepheshe obusha be-NGS obukwazile ukunciphisa izindleko zokulandelana nokwandisa umthamo. Ukulungiswa kwemitapo yolwazi ye-DNBSEQ kuhilela ukuhlukaniswa kwe-DNA, ukulungiswa kwe-ssDNA, kanye nokukhulisa i-rolling circle ukuthola ama-nanoball e-DNA (DNB). Lawa abese elayishwa endaweni eqinile bese elandelaniswa yi-combinatorial Probe-Anchor Synthesis (cPAS). Ubuchwepheshe be-DNBSEQ buhlanganisa izinzuzo zokuba nesilinganiso samaphutha esiphansi sokukhulisa nokusebenzisa amaphethini amaphutha aphezulu ngama-nanoball, okuholela ekulandeleni umthamo ophezulu kanye nokunemba.

Insizakalo yethu yokulandelelanisa amalabhulali eyenziwe ngaphambilini ivumela amakhasimende ukuthi alungiselele amalabhulali okulandelelanisa ama-Illumina avela emithonjeni eyahlukahlukene (i-mRNA, i-genome ephelele, i-amplicon, amalabhulali angu-10x, phakathi kwabanye), aguqulwa abe amalabhulali e-MGI emalabhorethri ethu ukuze ahlelwe ngokulandelana ku-DNBSEQ-T7, okuvumela inani eliphezulu ledatha ngezindleko eziphansi.

Xhumana nathi

Imininingwane Yesevisi

Umphumela Wedemo

Izici

●Ipulatifomu:I-MGI-DNBSEQ-T7

●Izindlela zokulandelanisa:I-PE150

●Ukudluliselwa kwemitapo yolwazi ye-Illumina ku-I-MGI:okuvumela ukulandelana kwenani elikhulu ledatha ngezindleko eziphansi.

●Ukulawulwa kwekhwalithi yemitapo yolwazi ngaphambi kokulandelana.

●Ukulandelanisa idatha ye-QC kanye nokulethwa:ukulethwa kombiko we-QC kanye nedatha eluhlaza ngefomethi ye-fastq ngemuva kokuhlukanisa ama-multiplex kanye nokuhlunga ama-Q30.

Izinzuzo

●Ukuguquguquka kwezinsizakalo zokulandelela:ikhasimende lingakhetha ukulandelana ngomzila noma inani ledatha.

●Ukukhishwa kwedatha okuphezulu:1500 Gb/umzila

●Ukulethwa kombiko we-QC wokulandelana:ngezilinganiso zekhwalithi, ukunemba kwedatha kanye nokusebenza okuphelele kwephrojekthi yokulandelana.

●Inqubo yokulandelana kwabantu abadala:ngesikhathi esifushane sokujika.

●Ukulawulwa Kwekhwalithi Okuqinile: sisebenzisa izidingo ze-QC eziqinile ukuqinisekisa ukulethwa kwemiphumela esezingeni eliphezulu njalo.

Izidingo Zesampula

	Inani Ledatha (X)	Ukugxila (qPCR/nM)	Ivolumu
Indlela Engaphelele	X ≤ 10 Gb	≥ 1nM	≥ 25 μl
	10 Gb < X ≤ 50 Gb	≥ 2 nM	≥ 25 μl
	50 Gb < X ≤ 100 Gb	≥ 3 nM	≥ 25 μl
	X > 100 Gb	≥ 4 nM
Indlela Eyodwa	Ngomzila Ngamunye	≥ 1.5 nM / Ichibi lomtapo wolwazi	≥ 25 μl / Ichibi lomtapo wolwazi

Ngaphezu kokuhlushwa kanye nenani eliphelele, iphethini efanele yokuphakama nayo iyadingeka.

Qaphela: Ukulandelana kwemizila yemitapo yolwazi ehlukahlukene kudinga ukukhushulwa kwe-PhiX ukuqinisekisa ukubizwa okuqinile kwesisekelo.

Sincoma ukuhambisa imitapo yolwazi ehlanganiswe kusengaphambili njengezibonelo. Uma udinga i-BMKGENE ukuthi yenze i-library pooling, sicela ubheke izidingo zomtapo wolwazi zokulandelana kwemizila engaphelele.

Usayizi Welabhulali (Imephu Ephakeme)

I-peak eyinhloko kufanele ibe ngaphakathi kuka-300-450 bp.

Amalabhulali kufanele abe ne-peak eyodwa enkulu, angabi nakungcola kwe-adaptha futhi angabi nama-primer dimers.

Ukuhamba Komsebenzi Wesevisi

Okwedlule: Ukusebenzisana kwe-Chromatin okusekelwe ku-Hi-C

Olandelayo: I-BMKMANU S3000_Spatial Transcriptome

Umbiko we-QC weLabhulali

Umbiko ngekhwalithi yomtapo wolwazi unikezwa ngaphambi kokulandelana, ukuhlola inani lomtapo wolwazi, kanye nokuqhekeka kwawo.

Ukulandelanisa umbiko we-QC

Ithebula 1. Izibalo mayelana nedatha yokulandelana.

I-ID Yesampula	I-BMKID	Ukufunda okungavuthiwe	Idatha Engavuthiwe (bp)	Ukufunda okuhlanzekile (%)	Q20(%)	Q30(%)	I-GC(%)
C_01	BMK_01	22,870,120	6,861,036,000	96.48	99.14	94.85	36.67
C_02	BMK_02	14,717,867	4,415,360,100	96.00	98.95	93.89	37.08