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I-Epigenetics

  • Chromatin Immunoprecipitation Sequencing (ChIP-seq)

    I-Chromatin Immunoprecipitation Sequencing (ChIP-seq)

    I-ChIP-Seq inikeza iphrofayili yofuzo olubanzi lwezinjongo ze-DNA zokuguqulwa kwe-histone, izici zokuloba, namanye amaprotheni ahlobene ne-DNA.Ihlanganisa ukukhetha kwe-chromatin immuno-precipitation (ChIP) ukuze kubuyiselwe izakhi ze-protein-DNA ezithile, namandla okulandelana kwesizukulwane esilandelayo (NGS) okulandelana okuphezulu komphumela we-DNA etholiwe.Ukwengeza, ngenxa yokuthi ama-protein-DNA complexes atholakala kumangqamuzana aphilayo, izingosi ezibophayo zingaqhathaniswa nezinhlobo zamangqamuzana nezicubu ezihlukene, noma ngaphansi kwezimo ezihlukene.Izicelo zisukela ekulawuleni okulotshiweyo kuye ezindleleni zokuthuthuka eziya ezindleleni zezifo nangale kwalokho.

    Ipulatifomu: Illumina NovaSeq 6000

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  • Whole genome bisulfite sequencing

    Ukulandelana okuphelele kwe-genome bisulfite

    I-DNA methylation endaweni yesihlanu ku-cytosine (5-mC) inethonya elibalulekile ekukhulumeni kofuzo kanye nomsebenzi wamaselula.Amaphethini e-methylation angavamile ahlotshaniswa nezimo nezifo eziningana, njengomdlavuza.I-WGBS isiphenduke indinganiso yegolide yokufunda i-genome-wide methylation ngokulungiswa kwesisekelo esisodwa.

    Ipulatifomu: Illumina NovaSeq6000

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  • Assay for Transposase-Accessible Chromatin with High Throughput Sequencing (ATAC-seq)

    I-Assay ye-Chromatin Efinyelelekayo Ye-Transposase Enokulandelanisa Okuphezulu Kokudlula (i-ATAC-seq)

    I-ATAC-seq iyindlela yokulandelanisa ephezulu yokuhlaziywa kokufinyeleleka kwe-chromatin ebanzi ye-genome, okubalulekile ekulawuleni i-epigenetic yomhlaba wonke ekukhulumeni kofuzo.Ama-adaptha alandelanayo afakwa ezifundeni ze-chromatin nge-hyperactive Tn5 transposase.Ngemuva kokukhulisa i-PCR, kwakhiwa umtapo wolwazi wokulandelanisa.Zonke izifunda ze-chromatin ezivulekile zingatholwa ngaphansi kwesimo sesikhathi esithile sesikhala, hhayi kuphela kumasayithi abophezelayo wesici sokuloba, noma indawo ethile elungisiwe ye-histone.

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  • Reduced Representation Bisulfite Sequencing (RRBS)

    I-Reduced Representation Bisulfite Sequencing (RRBS)

    Ucwaningo lwe-DNA methylation belulokhu luyisihloko esishisayo ocwaningweni lwezifo, futhi luhlobene eduze nokuvezwa kofuzo kanye nezici ze-pheno-typic.I-RRBS iyindlela enembile, ephumelelayo neyongayo yocwaningo lwe-DNA methylation.Ukunothiswa kwezifunda eziqhingini zomgqugquzeli kanye ne-CpG nge-enzymatic cleavage (Msp I), kuhlanganiswe nokulandelana kwe-Bisulfite, kunikeza ukutholwa kwe-DNA methylation yokuxazulula okuphezulu.

    Ipulatifomu: Illumina NovaSeq 6000

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Thumela umlayezo wakho kithi:

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