Izakhi Zofuzo Ezivela Emvelweni
Izinzuzo Zesevisi
UTakagi nabanye.,Ijenali yezitshalo, 2013
●Ukuhlaziywa okuphelele kwe-bioinformatic:ukuvumela ukuqagela kokuhlukahluka kwezakhi zofuzo, okubonisa amandla okuziphendukela kwemvelo kwezinhlobo, kanye nokwembula ubudlelwano obuthembekile be-phylogenetic phakathi kwezinhlobo ngethonya elincane lokuziphendukela kwemvelo okuguquguqukayo kanye nokuziphendukela kwemvelo okuhambisanayo
●Ukuhlaziywa okwenziwe ngokwezifiso okungakhethwa: njengokuqagela isikhathi sokwehluka kanye nesivinini ngokusekelwe ekushintsheni kwezinga le-nucleotide kanye nama-amino acid.
●Ubuchwepheshe Obubanzi kanye namarekhodi okushicilela: I-BMKGene iqoqe ulwazi olukhulu kumaphrojekthi ofuzo lwabantu kanye nokuziphendukela kwemvelo iminyaka engaphezu kwengu-15, ihlanganisa izinkulungwane zezinhlobo, njll. futhi inegalelo kumaphrojekthi aphezulu angaphezu kwe-1000 ashicilelwe ku-Nature Communications, Molecular Plants, Plant Biotechnology Journal, njll.
● Ithimba le-bioinformatics elinekhono eliphezulu kanye nomjikelezo omfushane wokuhlaziya: ngesipiliyoni esihle ekuhlaziyweni kwe-genomics okuthuthukisiwe, ithimba le-BMKGene lihlinzeka ngokuhlaziya okuphelele ngesikhathi sokushintsha okusheshayo.
● Usekelo Lwangemva Kokuthengisa:Ukuzibophezela kwethu kudlulela ngale kokuqedwa kwephrojekthi ngesikhathi senkonzo yezinyanga ezintathu ngemuva kokuthengisa. Phakathi nalesi sikhathi, sinikeza ukulandelwa kwephrojekthi, usizo lokuxazulula izinkinga, kanye nezikhathi ze-Q&A ukuze kuxazululwe noma yimiphi imibuzo ehlobene nemiphumela.
Imininingwane Yesevisi kanye nezidingo
| Uhlobo lokulandelana | Isikali sabantu esinconywayo | Isu lokulandelana kwezigaba | Izidingo ze-Nucleotide |
| Ukulandelana Kwezakhi Zofuzo Okuphelele | ≥ abantu abangu-30, kanye nabantu abangu-≥ abayi-10 eqenjini ngalinye elincane
| 10x | Ukugxila: ≥ 1 ng/ µL Inani eliphelele ≥ 30ng Ukuwohloka noma ukungcola okulinganiselwe noma okungenakho |
| I-Specific-Locus Amplified Fragment (SLAF) | Ukujula kwethegi: 10x Inani lamathegi: <400 Mb: Kunconywa i-WGS <1Gb: Amathegi angu-100K 1Gb >2Gb: Amathegi angu-300K Amathegi aphezulu angu-500k | Ukugxila ≥ 5 ng/µL Inani eliphelele ≥ 80 ng Ijeli le-Agarose: akukho noma ukuwohloka okulinganiselwe noma ukungcola
|
Ukugeleza Komsebenzi Wesevisi
Umklamo wokuhlola
Ukulethwa kwesampula
Ukwakhiwa komtapo wolwazi
Ukulandelana
Ukuhlaziywa kwedatha
Izinsizakalo zangemuva kokuthengisa
Isevisi ifaka phakathi ukuhlaziywa kwesakhiwo sabantu (isihlahla se-phylogenetic, i-PCA, ishadi lokwahlukaniswa kwabantu), ukuhlukahluka kwabantu, kanye nokukhethwa kwabantu (ukungalingani kokuxhumana, ukukhethwa kokukhethwa kwezindawo ezizuzisayo). Isevisi ingahlanganisa nokuhlaziywa okwenziwe ngokwezifiso (isb. isikhathi sokwehluka, ukugeleza kwezakhi zofuzo).
*Imiphumela yedemo eboniswe lapha yonke ivela kuma-genome ashicilelwe nge-BMKGENE
1. Ukuhlaziywa kokuziphendukela kwemvelo kuqukethe ukwakhiwa kwesihlahla se-phylogenetic, isakhiwo sabantu kanye ne-PCA ngokusekelwe ekuhlukeni kwezakhi zofuzo.
Umuthi we-phylogenetic umele ubudlelwano be-taxonomic kanye nokuziphendukela kwemvelo phakathi kwezinhlobo ezinokhokho ofanayo.
I-PCA ihlose ukubona ngeso lengqondo ukusondelana phakathi kwabantu abangaphansi kwayo.
Isakhiwo sabantu sibonisa ukuba khona kwenani elincane labantu elihlukile ngokwezakhi zofuzo ngokwemvamisa ye-allele.
UChen, nabanye,I-PNAS, 2020
2. Ukusula okukhethayo
Ukuhlunga okukhethiwe kubhekisela enkambisweni lapho kukhethwa khona indawo enenzuzo futhi imvamisa yezindawo ezingathathi hlangothi ezixhunyiwe iyanda futhi lezo zezindawo ezingaxhunywanga ziyancipha, okuholela ekunciphiseni kwezindawo.
Ukutholwa kwe-genome yonke ezindaweni ezikhethiwe zokusula kucutshungulwa ngokubala inkomba yezakhi zofuzo zabantu (π，Fst, Tajima's D) yazo zonke i-SNP ngaphakathi kwefasitela elishelelayo (100 Kb) esinyathelweni esithile (10 Kb).
Ukuhlukahluka kwe-Nucleotide(π）
U-D kaTajima
Inkomba yokulungisa (Fst)
UWu, nabanye.,Isitshalo Se-Molecular, 2018
3. Ukugeleza Kwezakhi Zofuzo
UWu, nabanye.,Isitshalo Se-Molecular, 2018
4. Umlando wabantu
UZhang, nabanye.,I-Ecology Yemvelo Nokuziphendukela Kwemvelo, 2021
5. Isikhathi sokwehlukana
UZhang, nabanye.,I-Ecology Yemvelo Nokuziphendukela Kwemvelo, 2021
Hlola intuthuko eyenziwe yizinsizakalo ze-BMKGene zokuguqula izakhi zofuzo ngokusebenzisa iqoqo lezincwadi ezikhethiwe:
Hassanyar, AK et al. (2023) 'Ukutholakala Kwezimpawu Zama-Molecular ze-SNP kanye Nezakhi Zofuzo Ezingakhethwa Ezihlobene Nokumelana Negciwane Le-Sacbrood ku-Apis cerana cerana Larvae Ngokulandelana Kwezakhi Zofuzo Eziphelele',Ijenali yomhlaba wonke yesayensi yama-molecular, 24(7). doi: 10.3390/IJMS24076238.
UChai, J. et al. (2022) 'Ukutholwa kwensala enkulu yaseShayina yasendle, emsulwa ngofuzo kudala amathuba amasha okulondolozwa',Ucwaningo Lwezilwane, 2022, uMqulu 43, Udaba 3, Amakhasi: 469-480, 43(3), amakhasi 469–480. doi: 10.24272/J.ISSN.2095-8137.2022.101.
UHan, M. et al. (2022) 'Iphethini ye-Phylogeographical kanye nomlando wokuguquka kwabantu bomdabu u-Elymus sibiricus L. e-Qinghai-Tibetan Plateau',Imingcele Yesayensi Yezitshalo, 13, ikhasi 882601. doi: 10.3389/FPLS.2022.882601/BIBTEX.
UWang, J. et al. (2022) 'Ukuqonda kwe-genomic ekuguqukeni kwe-longan kusuka ekuhlanganisweni kwe-genome yezinga le-chromosome kanye ne-genomics yabantu yokuhlanganiswa kwe-longan',Ucwaningo Lwezolimo, 9. doi: 10.1093/HR/UHAC021.
