I-TGuide Smart Magnetic Plant RNA Kit
Inombolo yekhathalogi / iphakheji
| Ikati. cha | ID | Inombolo ye-preps |
| 4993552(ikhatriji) | I-DP662-DE | 48 |
Umhlahlandlela wokukhetha i-reagent ye-TGuide S16
| I-Nuclei Acid | Uhlobo lwesampula | Igama Lomkhiqizo | Usayizi wokupakisha (preps) Ikhatriji/ ipuleti | Inombolo yekati. Ikhatriji/ipuleti |
| I-DNA | Izicubu Zezilwane | I-TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| I-DNA | Isitshalo Nembewu | I-TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| I-DNA | Umhlabathi/Indle | TGuide Smart Soil /Stool DNA Kit | 48 | 4993549 |
| I-DNA | Imikhiqizo ye-Gel ne-PCR | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| I-DNA | Igazi Eliphelele | I-TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| I-DNA | Iseli/I-Swab/Izindawo Ezomile, njll | I-TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| I-RNA | Igazi/Iseli/Izicubu | I-TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| I-RNA | Isitshalo Nembewu | I-TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| I-DNA/RNA | Uketshezi Lwamahhala Lweseli (iSerum, njll) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Isingeniso somkhiqizo
Hlanza isivuno esiphezulu, ukuhlanzeka okuphezulu, i-RNA yekhwalithi ephezulu egazini/iseli/izicubu.
Awekho ama-reagents anobuthi njenge-phenol/chloroform aqukethwe
I-RNA etholiwe inokuhlanzeka okuphezulu futhi ingasetshenziselwa ngokuqondile ukutholwa kwe-chip, ukulandelana kwe-high-throughput nokunye ukuhlola.
Izici
Kulula kakhulu ukusebenzisa
Awukho umsebenzi owengeziwe wamapayipi.
Ivolumu ye-elution ingalungiswa njengoba kudingeka.
Ukufakwa nokusebenza kudinga ukuqeqeshwa okuncane. Ngezinhlelo ezifakwe ngaphambilini, khipha i-cartridge, khetha umthetho olandelwayo bese uqalisa ukuhlola kwakho. Izinhlelo sezilungele ukusetshenziswa, futhi zingenziwa ngendlela oyifisayo.
Ama-reagents alayishwe ngaphambili kanye nezinto ezisetshenziswayo ezilahlwayo.
Ukuphathwa kwamakhemikhali kuqedwa ngezinga elikhulu ukuze kuqinisekiswe ukuphepha kokusebenza.
Umphumela wedemo
Isizinda sikakolweni sempande ye-RNA
Usayizi wesampula: 100 mg
Ukwelashwa kwangaphambili okuyisampula: ukugaya nge-nitrogen ewuketshezi noma ukwelashwa kwezicubu ezisezingeni eliphansi ze-homogenizer
Ijeli ye-Agarose :2%(TAE)
Ivolumu yokulayisha: 2 μl
Umaka: Umaka IV, TIANGEN
Umphumela wokuhlola: usebenzisa i-TGuide S16 etholwe nge-reagent 4993552 ukuze kukhishwe ngokuzenzakalelayo ingqikithi ye-RNA yezimpande zikakolweni kunesivuno esihle nokuhlanzeka okuphezulu. Kulesi sivivinyo, cishe i-20 μg ye-nucleic acid ikhishwe ezimpandeni zikakolweni ezingu-100 mg, ne-OD260 / OD280 ezungeze i-2.0 ~ 2.1, ne-OD260 / OD230＞2.0.
Isamba se-RNA esikhishwe kumasampula ezitshalo ahlukene
Usayizi wesampula: 100 mg
Ukwelashwa kwangaphambili okuyisampula: i-homogenizer enezinga lokushisa eliphansi
Ijeli ye-Agarose :1% (TAE)
Ivolumu yokulayisha: 1 μl
M: Umaka III, TIANGEN
1-4: imbewu yommbila 5-8: amaqabunga ommbila 9-12: izimpande zeklabishi 13-16: ubhontshisi wesoya
Amasampuli amabili okuqala akhishwe ngekhithi yokukhipha ikholomu espin futhi amasampula okugcina akhishwe yi-TGuide S16.
Umphumela wokuhlolwa: isivuno esikhishwayo sesitshalo se-RNA nge-TGuide S16 esine-reagent 4993552 siyalingana noma singcono kunalokho ngekhithi yokukhipha ikholomu elispin. Ubumsulwa nobuqotho kuyafana. Ngakho-ke, i-TGuide S16 ingasetshenziselwa ukukhipha isamba sesitshalo se-RNA esikhundleni sesixazululo sokukhipha esisekelwe kukholomu.
