Imikhiqizo

I-Fungal Genome

I-Biomarker Technologies ihlinzeka ngenhlolovo ye-genome, i-genome enhle kanye ne-pene-complete genome yefungal kuye ngenhloso ethile yocwaningo.Ukulandelana kwe-genome, ukuhlanganisa kanye nesichasiselo esisebenzayo kungafinyelelwa ngokuhlanganisa ukulandelana kwesizukulwane Esilandelayo + Ukulandelana kwesizukulwane sesithathu ukuze kuzuzwe ukuhlanganiswa kwegenome kwezinga eliphezulu.Ubuchwepheshe be-Hi-C bungaphinda buqashwe ukuze kube lula ukuhlangana kwe-genome ezingeni le-chromosome.

Inkundla:I-PacBio Sequel II

I-Nanopore PromethION P48

I-Illumina NovaSeq Platform

Xhumana nathi

Imininingwane Yesevisi

Imiphumela yedemo

Izinzuzo Zesevisi

● Amasu amaningi okulandelana atholakalayo ezinhlosweni ezihlukene zocwaningo

● Unolwazi oluphezulu ekuhlanganiseni kofuzo lwesikhunta okuhlanganiswe i-pene-complete genome engaphezu kuka-10,000.

● Ithimba losekelo lobuchwepheshe ngemva kokuthengisa elenza izidingo ezithile zocwaningo.

Imininingwane Yesevisi

Isevisi	Isu Lokulandelanisa	Ikhwalithi iqinisekisiwe	Isikhathi sokujika esilinganiselwe
Ibalazwe elihle le-fungal	I-Illumina 50X+Nanopore 100X	I-Contig N50≥2 Mb	35 izinsuku zokusebenza
Ibalazwe elihle le-fungal	I-PacBio HiFi 30X	I-Contig N50≥2 Mb	35 izinsuku zokusebenza
Imephu ye-fungal pene-complete	I-Illumina 50X+Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X	Isilinganiso se-Chromosome anchoring>90%	45 izinsuku zokusebenza

I-Bioinformatics ihlaziya

● Ukulawulwa kwekhwalithi yedatha eluhlaza

● Ukuhlanganiswa kwe-genome

● Ukuhlaziywa kwengxenye ye-genome

● Isichasiselo somsebenzi wofuzo

● Ukuhlaziywa kwe-genomic okuqhathanisayo

Izidingo Zesampula Nokulethwa

Izimfuneko zesampula:

NgobaIzingcaphuno ze-DNA:

Uhlobo lwesampula	Inani	Ukugxila	Ubumsulwa
Izingcaphuno ze-DNA	＞ 1.2 μg	＞ 20 ng/μl	OD260/280= 1.6-2.5

Okwamasampula ezicubu:

Uhlobo lwesampula

Ukwelashwa okuyisampula okunconyiwe

Isampula yokugcina nokuthunyelwa

Ukhunta Unicellular

Bheka imvubelo ngaphansi kwe-microscope futhi uyiqoqe esigabeni sayo se-exponential

Dlulisa isiko (eliqukethe cishe amaseli angu-3-4.5e9) ku-1.5 noma 2 ml eppendorf.(Qhubekela eqhweni)

I-Centrifuge ishubhu iminithi elingu-1 ku-14000 g ukuqoqa amabhaktheriya futhi ususe i-supernatant ngokucophelela.

Vala ishubhu bese ufriza amagciwane ku-nitrogen ewuketshezi okungenani amahora angu-1-3.Gcina ishubhu ku -80 ℃ esiqandisini.

Friza amasampula ku-nitrogen ewuketshezi amahora angu-3-4 futhi uwagcine ku-nitrogen ewuketshezi noma -80 degree ekubhukeni isikhathi eside.Kudingeka isampula yokuthunyelwa ngeqhwa elomile.

Isikhunta esikhulu

Kunconywa ukuthi izicubu ezisesigabeni sokukhula zikhule.

Hlanza izicubu ngamanzi angenayo i-endotoxin, bese u-70% we-ethonal.

Londoloza isampula kumashubhu e-cryo.