Ukusebenzisana kwe-Chromatin okusekelwe ku-Hi-C
Izici Zesevisi
● Ukulandelana kwe-Illumina NovaSeq nge-PE150.
● Isevisi idinga amasampula ezicubu, esikhundleni sama-nucleic acid akhishwe, ukuze axhumane ne-formaldehyde futhi alondoloze ukusebenzisana kwe-DNA-protein.
● Ukuhlolwa kwe-Hi-C kuhilela ukuvinjelwa nokulungiswa kokuphela kweziphetho ezinamathelayo nge-biotin, kulandelwe ukujikeleziswa kweziphetho ezibuthuntu eziphumayo ngenkathi kulondolozwa ukusebenzisana. I-DNA ibe isidonswa phansi ngama-streptavidin beads bese ihlanzwa ukuze kulungiselelwe umtapo wolwazi olandelayo.
Izinzuzo Zesevisi
●Umklamo we-Enzyme Wokukhawulela Okufanele: ukuqinisekisa ukusebenza kahle kwe-Hi-C okuphezulu ezinhlotsheni ezahlukene ngemibhangqwana yokusebenzisana evumelekile efinyelela ku-93%.
●Amarekhodi Obuchwepheshe Obubanzi kanye Nokushicilela:I-BMKGene inokuhlangenwe nakho okukhulu ngamaphrojekthi okulandelana kwe-Hi-C angaphezu kuka-2000 avela ezinhlotsheni ezahlukene ezingu-800 kanye namalungelo obunikazi ahlukahlukene. Amacala angaphezu kwe-100 ashicilelwe ane-accumulative impact factor engaphezu kuka-900.
●Ithimba le-Bioinformatics elinekhono eliphezulu:ngama-patents angaphakathi kanye namalungelo okushicilela esofthiwe okuhlola kwe-Hi-C kanye nokuhlaziywa kwedatha kanye nesofthiwe yedatha yokubona ethuthukiswe ngokwayo.
●Usekelo Lwangemva Kokuthengisa:Ukuzibophezela kwethu kudlulela ngale kokuqedwa kwephrojekthi ngesikhathi senkonzo yezinyanga ezintathu ngemuva kokuthengisa. Phakathi nalesi sikhathi, sinikeza ukulandelwa kwephrojekthi, usizo lokuxazulula izinkinga, kanye nezikhathi zemibuzo nezimpendulo ukuze kuxazululwe noma yimiphi imibuzo ehlobene nemiphumela.
●Isichasiselo Esiphelele: sisebenzisa izizindalwazi eziningi ukuze sichaze izakhi zofuzo ngokusebenza ngokuhlukahluka okubonakalayo futhi senze ukuhlaziywa kokunotha okuhambisanayo, sinikeze ukuqonda kumaphrojekthi amaningi ocwaningo.
Imininingwane Yesevisi
| Umtapo Wolwazi | Isu Lokulandelana | Ukukhishwa kwedatha okunconyiwe | Isixazululo Sesignali ye-Hi-C |
| Umtapo wolwazi we-Hi-C | I-Illumina PE150 | Iluphu ye-Chromatin: 150x I-TAD: 50x | I-Chromatin Loop: 10Kb I-TAD: 40Kb |
Izidingo Zesevisi
| Uhlobo lwesampula | Inani elidingekayo |
| Izicubu zezilwane | ≥2g |
| Igazi Eliphelele | ≥2mL |
| Isikhunta | ≥1g |
| Izicubu ezincane zezitshalo | 1g/aliquot, kunconywa ama-aliquot angu-2-4 |
| Amaseli Akhuliswe | ≥1x107 |
Kufaka phakathi Ukuhlaziywa Okulandelayo:
● Idatha eluhlaza QC;
● Ukumapha kanye nomtapo wolwazi we-Hi-C QC: ama-pairs okuxhumana avumelekile kanye nama-Interaction Decay Exponents (ama-IDE);
● Ukuphrofayili Yokuxhumana Okubanzi Kwe-Genome: ukuhlaziywa kwe-cis/trans kanye nemephu yokuxhumana ye-Hi-C;
● Ukuhlaziywa kokusatshalaliswa kwe-A/B compartment;
● Ukuhlonza ama-TAD nama-chromatin loops;
● Ukuhlaziywa kokwehluka kwezinto zesakhiwo se-chromatin se-3D phakathi kwamasampula kanye nesichasiselo esisebenzayo esihambisanayo sezakhi zofuzo ezihambisanayo.
Ukusatshalaliswa kwe-Cis kanye ne-trans proportion
Imephu yokushisa yokusebenzisana kwama-chromosome phakathi kwamasampula
Ukusatshalaliswa kwezakhi ze-A/B ngofuzo lonke
Ukusatshalaliswa kwe-genome kwama-chromatin loops
Ukuboniswa kwe-TAD
Hlola intuthuko yocwaningo eyenziwe yizinsizakalo zokulandelana kwe-BMKGene ze-Hi-C ngokusebenzisa iqoqo lezincwadi ezikhethiwe.
UMeng, T. et al. (2021) 'Ukuhlaziywa kwe-multi-omics okuhlanganisiwe okuqhathanisayo kukhomba i-CA2 njengesisulu esisha se-chordoma',I-Neuro-Oncology, 23(10), amakhasi 1709-1722. doi: 10.1093/NEUONC/NOAB156.
Xu, L. et al. (2021) 'Ukungahleleki kwe-3D kanye nokuhlela kabusha i-genome kunikeza ukuqonda nge-pathogenesis ye-NAFLD ngokuhlanganiswa kwe-Hi-C, i-Nanopore, kanye ne-RNA',I-Acta Pharmaceutica Sinica B, 11(10), amakhasi 3150-3164. doi: 10.1016/J.APSB.2021.03.022.
