Imikhiqizo

I-Metabolomics

I-metabolome ingumkhiqizo ogcina phansi womfula we-genome futhi iqukethe isamba esiphelele sawo wonke ama-molecule anesisindo esiphansi (ama-metabolites) kuseli, izicubu, noma into ephilayo.I-Metabolomics ihlose ukukala ububanzi obubanzi bama-molecule amancane kumongo wezisusa ze-physiological noma izimo zezifo.Izindlela ze-Metabolomics ziwela emaqenjini amabili ahlukene: i-metabolomics engahlosiwe, ukuhlaziya okuhlosiwe okuhlosiwe kwazo zonke izihlaziyi ezingalinganiseka kusampula okuhlanganisa namakhemikhali angaziwa kusetshenziswa i-GC-MS/LC-MS, kanye ne-metabolomics eqondisiwe, ukukalwa kwamaqembu achaziwe aphawulwe ngamakhemikhali kanye ama-metabolites ane-biochemically.

Xhumana nathi

Imininingwane Yesevisi

Izidingo Zesampula

I-metabolomics engahlosiweIzinhlobo zamasampuli		Isampula enconyiweinani	Ukuphindaphinda Kwebhayoloji
LI-C-MS/GC-MSi-metabolomics engahlosiwe	Izicubu	200mg	Isitshalo ≥6; Isilwane ≥10; Umuntu ≥30;
	I-Plasma/serum	200ul
	Okuqukethwe kwe-Fecal/Amathumbu	150mg
	Amaseli	1*10⁶
	umchamo	500ul
	I-Rumen fluid	1ml
I-metabolomics ehlosiweIzinhlobo zamasampuli		Inani lesampula elinconyiwe	Ukuphindaphinda Kwebhayoloji
I-Phytohormone/Tryptophane/I-Energy Metabolism/ lipids oxidized/Carotenoid;i-carotinoid	Izicubu	500mg	Isitshalo ≥3; Isilwane ≥6;
	I-Plasma/serum	500ul
	I-Fecal	1000mg
	Amaseli	1*10⁷

Imininingwane Yesevisi

I-metabolomics engahlosiwe:
1.Ubuchwepheshe obuvuthiwe nobuzinzile, ukulungiswa okuphezulu nokukhetha okuhle
2. Gcwalisa imininingwane egciniwe;
3. Ifanele ukuhlaziywa kwamatrices ayinkimbinkimbi, inikeza ulwazi olunzulu ekuhlaziyeni okukodwa;

I-metabolomics ehlosiwe:
1.Sebenzisa ukuqinisekiswa okujwayelekile kwe-isotopu ukuze uthuthukise ukunemba kwekhwalithi nobuningi bezinto;
I-2.Isistimu yokulawula ikhwalithi ukuze kuqinisekiswe ukwethembeka kwedatha.