Ukulandelana kwe-Metatranscriptome
Izici Zesevisi
● Ukuncipha kwe-rRNA kulandelwa ukulungiswa komtapo wezincwadi we-mRNA oqondisiwe.
● Ukulandelana kwezithombe ku-Illumina NovaSeq.
Izinzuzo Zesevisi
●Funda Izinguquko Zemiphakathi Eyinkimbinkimbi Yezilokazane Ezincane:Lokhu kwenzeka ezingeni lokubhalwa kwezakhi zofuzo futhi kuhlola izakhi zofuzo ezintsha ezingaba khona.
●Ukuchaza Ukusebenzisana Komphakathi Wezilokazane Ezincane Nomnikazi Wendawo Noma Imvelo.
●Ukuhlaziywa Okuphelele Kwe-BioinformaticLokhu kunikeza ukuqonda mayelana nokwakheka kwe-taxonomic yomphakathi kanye nokusebenza kwayo, kanye nokuhlaziywa kokubonakaliswa kwezakhi zofuzo ezihlukile.
●Isichasiselo Esibanzi Sezakhi Zofuzo:Ukusebenzisa izizindalwazi zomsebenzi wezakhi zofuzo ezibuyekeziwe ukuze kutholakale ulwazi olunolwazi mayelana nokubonakaliswa kwezakhi zofuzo zemiphakathi yamagciwane.
●Usekelo Lwangemva Kokuthengisa:Ukuzibophezela kwethu kudlulela ngale kokuqedwa kwephrojekthi ngesikhathi senkonzo yezinyanga ezintathu ngemuva kokuthengisa. Phakathi nalesi sikhathi, sinikeza ukulandelwa kwephrojekthi, usizo lokuxazulula izinkinga, kanye nezikhathi zemibuzo nezimpendulo ukuze kuxazululwe noma yimiphi imibuzo ehlobene nemiphumela.
Imininingwane Yesevisi
| Ipulatifomu yokulandelana | Isu Lokulandelana | Kunconywa idatha | Ukulawulwa Kwekhwalithi Yedatha |
| I-Illumina NovaSeq | I-PE150 | 12Gb | Q30≥85% |
Izidingo Zesampula
| Ukugxila (ng/µL) | Inani eliphelele (µg) | Ivolumu (µL) | OD260/280 | OD260/230 | I-RIN |
| ≥50 | ≥1.0 | ≥20 | 1.8-2.0 | 1.0-2.5 | ≥6.5 |
Ukugeleza Komsebenzi Wesevisi
Ukulethwa kwesampula
Ukwakhiwa komtapo wolwazi
Ukulandelana
Ukuhlaziywa kwedatha
Izinsizakalo zangemuva kokuthengisa
Kufaka phakathi ukuhlaziywa okulandelayo:
● Ukulawula Ikhwalithi Yedatha Ngokulandelana
● Ukuhlanganiswa Kombhalo
● Isichasiselo se-Taxonomic kanye Nobuningi
● Isichasiselo Esisebenzayo Nobuningi
● Ukuhlolwa Kokulinganiselwa Kokuzibonakalisa kanye Nokuhlukaniswa Kokuhluzwa
Ukusatshalaliswa kwe-taxonomic kwesampula ngayinye:
Ukuhlaziywa kokwehlukahlukana kwe-Beta: UPGMA
Isichasiselo esisebenzayo – ubuningi be-GO
Ubuningi be-taxonomy ehlukene - LEFSE
Hlola intuthuko eyenziwe yizinsizakalo zokulandelana kwe-meta transcriptomics ze-BMKGene ngokusebenzisa iqoqo lezincwadi ezikhethiwe.
ULu, Z. nabanye (2023) 'Ukubekezelelana kwe-asidi kwamagciwane asebenzisa i-lactate ohlelo lwe-Bacteroidales kunegalelo ekuvimbeleni i-ruminal acidosis ezimbuzini ezijwayele ukudla okugxile kakhulu',Ukudla Kwezilwane, 14, amakhasi 130-140. doi: 10.1016/J.ANINU.2023.05.006.
Ingoma, Z. et al. (2017) 'Ukuvula i-microbiota esebenzayo eyinhloko ekuvuthweni kwesimo esiqinile ngokwendabuko ngama-amplicons aphezulu kanye nokulandelana kwe-metatranscriptomics',Imingcele ku-Microbiology, 8(JUL). doi: 10.3389/FMICB.2017.01294/FULL.
UWang, W. et al. (2022) 'Ama-Mycovirus Amasha Atholakale Ku-Metatranscriptomics Survey of the Phytopathogenic Alternaria Fungus',Amagciwane, 14(11), ikhasi 2552. doi: 10.3390/V14112552/S1.
UWei, J. et al. (2022) 'Ukuhlaziywa kwe-metatranscriptome okuhambisanayo kwembula ukuwohloka kwama-metabolite esibili ezitshalo yizimbungulu kanye nezimpawu zazo zamathumbu',Ama-molecule I-Ecology, 31(15), amakhasi 3999-4016. doi: 10.1111/MEC.16557.
