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I-Metagenomic Sequencing (NGS)
I-Metagenome ibhekisela eqoqweni lengqikithi yezakhi zofuzo zomphakathi oxubile wezinto eziphilayo, njenge-metagenome yemvelo, i-metagenome yomuntu, njll. Iqukethe ama-genome akho kokubili amagciwane amancane alinywayo nangatshalwa.Ukulandelana kwe-Metagenomic iyithuluzi lamangqamuzana elisetshenziselwa ukuhlaziya izinto ezixubile ze-genomic ezikhishwe kumasampula emvelo, okunikeza ulwazi oluningiliziwe ngokuhlukahluka kwezinhlobo kanye nobuningi, ukwakheka kwabantu, ubudlelwano be-phylogenetic, izakhi zofuzo ezisebenzayo kanye nenethiwekhi yokuxhumana nezici zemvelo.
Inkundla:Illumina NovaSeq6000
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Ukulandelana kwe-Metagenomic-Nanopore
I-Metagenomics iyithuluzi lamangqamuzana elisetshenziselwa ukuhlaziya izinto ezixubile ze-genomic ezikhishwe kumasampula emvelo, ezihlinzeka ngolwazi oluningiliziwe ngokuhlukahluka kwezinhlobo nobuningi, ukwakheka kwabantu, ubudlelwano be-phylogenetic, izakhi zofuzo ezisebenzayo kanye nenethiwekhi yokuxhumana enezici zemvelo, njll. I-Nanopore sequencing platforms isanda kwethulwa kwizifundo ze-metagenomic.Ukusebenza kwayo okuhle kakhulu kubude bokufunda kuthuthukise kakhulu ukuhlaziywa kwe-metagenomic yokusakaza, ikakhulukazi ukuhlanganiswa kwe-metagenome.Ngokuthatha izinzuzo zobude bokufunda, ucwaningo lwe-metagenomic olusekelwe ku-Nanopore luyakwazi ukuzuza umhlangano oqhubekayo uma uqhathaniswa ne-shot-gun metagenomics.Kushicilelwe ukuthi i-metagenomics esekwe e-Nanopore ikhiqize ngempumelelo ama-genomes amagciwane aphelele futhi avaliwe avela kuma-microbiomes (Moss, EL, et. al,I-Nature Biotech, 2020)
Inkundla:I-Nanopore PromethION P48
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16S/18S/ITS Amplicon Sequencing-PacBio
Iyunithi engaphansi ku-16S kanye ne-18S rRNA equkethe zombili izifunda ezilondolozwe kakhulu neziguquguqukayo izigxivizo zeminwe zemolekyuli ephelele yokuhlonza izinto eziphilayo ze-prokaryotic ne-eukaryotic.Ngokusebenzisa ithuba lokulandelanisa, lawa ma-amplicon angaqondiswa ngokusekelwe ezingxenyeni ezilondoloziwe futhi izifunda eziguquguqukayo zingabonakaliswa ngokugcwele ukuhlonza i-microbial enomthelela ocwaningweni oluhlanganisa ukuhlaziywa kwe-microbial diversity, taxonomy, phylogeny, njll. I-molecule ye-Single-real-time(SMRT) ) ukulandelana kweplathifomu ye-PacBio kwenza kutholwe ukufundwa okude okunembe kakhulu, okungahlanganisa ama-amplicon anobude obugcwele (cishe u-1.5 KB).Ukubuka okubanzi kwendawo yofuzo kuthuthukise kakhulu ukulungiswa kwesichasiselo sezinhlobo kubhaktheriya noma emphakathini wesikhunta.
Inkundla:I-PacBio Sequel II
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16S/18S/ITS Amplicon Sequencing-NGS
Ukulandelana kwe-amplicon ye-16S/18S/ITS kuhloswe ekudaluleni i-phylogeny, taxonomy, kanye nezinhlobo eziningi zezilwane emphakathini we-microbial ngokuphenya imikhiqizo ye-PCR yomaka bofuzo abagcina indlu equkethe kokubili izingxenye ezixoxwa kakhulu nezingaguquguquki.Ukwethulwa kwalezi zigxivizo zeminwe zamangqamuzana aphelele ngu-Woeses et al,(1977) kunika amandla ukuhlukaniswa kwephrofayili ye-microbiome.Ukulandelana kwe-16S (ibhaktheriya), i-18S (isikhunta) kanye ne-Internal transcribed spacer(ITS, isikhunta) kuvumela ukukhonjwa kwazo zombili izinhlobo zezinhlobo eziningi kanye nezinhlobo ezingavamile nezingaziwa.Lobu buchwepheshe sebuphenduke ithuluzi elisetshenziswa kabanzi futhi elikhulu ekuhlonzeni ukwakheka kwe-microbial ehlukile ezindaweni ezahlukahlukene, njengomlomo womuntu, amathumbu, indle, njll.
Inkundla:Illumina NovaSeq6000
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Ukulandelana kabusha kweGenome yebhaktheriya kanye nefungal
Ukulandelanisa kabusha i-genome yegciwane kanye nesikhunta kuyithuluzi elibalulekile lokuqedela ama-genomes amagciwane aziwayo nesikhunta, kanye nokuqhathanisa ama-genome amaningi noma ukwenza imephu yezakhi zezinto eziphilayo ezintsha.Kubaluleke kakhulu ukulandelana kwawo wonke ama-genome e-bacterium kanye nesikhunta ukuze kukhiqizwe ama-genome ayizethenjwa anembile, ukwenza ukuhlonza ama-microbial kanye nezinye izifundo zokuqhathanisa zofuzo.
Ingxenyekazi: Illumina NovaSeq 6000
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I-Fungal Genome
I-Biomarker Technologies ihlinzeka ngenhlolovo ye-genome, i-genome enhle kanye ne-pene-complete genome yefungal kuye ngenhloso ethile yocwaningo.Ukulandelana kwe-genome, ukuhlanganisa kanye nesichasiselo esisebenzayo kungafinyelelwa ngokuhlanganisa ukulandelana kwesizukulwane esilandelayo + Ukulandelana kwesizukulwane sesithathu ukuze kuzuzwe ukuhlanganiswa kofuzo lwezinga eliphezulu.Ubuchwepheshe be-Hi-C bungaphinda buqashwe ukuze kube lula ukuhlangana kwe-genome ezingeni le-chromosome.
Inkundla:I-PacBio Sequel II
I-Nanopore PromethION P48
I-Illumina NovaSeq 6000
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Amagciwane Aphelele I-Genome
I-Biomarker Technologies ihlinzeka ngensizakalo yokulandelanisa ekwakheni i-genome ephelele yebhaktheriya engenasikhala esiyiziro.Ukuhamba komsebenzi okuyinhloko kwamagciwane ukwakhiwa okuphelele kofuzo kufaka phakathi ukulandelana kwesizukulwane sesithathu, ukuhlanganisa, isichasiselo esisebenzayo kanye nokuhlaziya okuthuthukisiwe kwe-bioinformamatic okufeza izinhloso ezithile zocwaningo.Iphrofayili ebanzi yofuzo lwebhaktheriya inika amandla ukudalulwa kwezinqubo ezibalulekile ezikhona ngaphansi kwezinqubo zabo zebhayoloji, okungase futhi kunikeze ireferensi ebalulekile yocwaningo lwe-genomic ezinhlotsheni eziphakeme ze-eukaryotic.
Inkundla:I-Nanopore PromethION P48 + Illumina NovaSeq 6000
I-PacBio Sequel II
