mRNA-seq (NGS) – De novo

Ukulandelana kwe-mRNA kuvumela ukuprofayilisha yonke imibhalo ye-mRNA kumaseli ngaphansi kwezimo ezithile futhi ubuchwepheshe obusetshenziswa kabanzi ezindaweni ezahlukene zocwaningo. Ipayipi le-BMKCloud De novo mRNA-seq lenzelwe ukuhlaziya imitapo yolwazi yokulandelelana kwe-poly-A lapho kungekho genome yokubhekisela etholakalayo. Ipayipi liqala ngokulawula ikhwalithi, lilandelwe yide novoukuhlanganiswa kwe-transcript kanye nokukhethwa kwesethi ye-unigene. Ukuhlaziywa kwesakhiwo se-unigene kubikezela ukulandelana kwekhodi (i-CDS) kanye nokuphindaphinda okulula kwe-sequence (i-SSR). Ngemuva kwalokho, ukuhlaziywa kokubonakaliswa komehluko kuthola izakhi zofuzo ezivezwe ngokuhlukile (ama-DEG) phakathi kwezimo ezivivinyiwe, kulandelwe isichasiselo esisebenzayo kanye nokucebisa ama-DEG ukuze kukhishwe ukuqonda kwezinto eziphilayo.