I-Nanopore yobude obugcwele be-transcriptome

Ukulandelana kwe-transcriptome ye-Nanopore kuyindlela enamandla yokulandelana kwe-cDNA yobude obugcwele, ukuhlonza nokulinganisa ngokunembile ama-isoforms e-transcript. I-BMKCloud Nanopore Full-Length Transcriptome Pipeline iklanyelwe ukuhlaziya idatha ye-RNA-Seq ekhiqizwe epulatifomu ye-Nanopore ngokumelene ne-genome yokubhekisela esezingeni eliphezulu enezincazelo ezinhle, ihlinzeka ngokuhlaziywa kwekhwalithi kanye nokwenani kokubili ezingeni le-gene kanye ne-transcript. Ngemuva kokulawulwa kwekhwalithi, kutholakala izilandelana ze-non-chimeric (FLNC) zobude obugcwele futhi izilandelana zokuvumelana zibekwe ku-genome yokubhekisela ukuze kususwe izilandelo ezingafuneki. Kusukela kule sethi ye-transcript, ukuvezwa kuyalinganiswa futhi izakhi zofuzo ezivezwe ngokuhlukile futhi izilandelo ziyahlonzwa futhi zichazwe ngokusebenza. Iphayiphi lihlanganisa nokuhlaziywa kwe-alternative polyadenylation (APA), ukuhlaziywa kwe-alternative splicing, ukuhlaziywa kwe-simple sequence repeat (SSR), ukubikezela kwe-lncRNA kanye nezinhloso ezihambisanayo, ukubikezela izilandelo zekhodi (CDS), ukuhlaziywa komndeni wezakhi zofuzo, ukuhlaziywa kwezinto zokubhala, ukubikezela izakhi zofuzo ezintsha kanye nokuchazwa okusebenzayo kwezilandelo.