I-transcriptome ephelele ye-Pacbio

I-PacBio Full-Length Transcriptome Sequencing, i-Isoseq, ivumela ukuhlonza okunembile kwama-isoforms e-transcript, ikhanyisela i-polyadenylation ehlukile kanye ne-splicing, futhi iholele ekuhlaziyweni kokubonakaliswa kwezakhi zofuzo okunembe kakhudlwana. Ipayipi le-BMKCloud PacBio Full-Length Transcriptome lenzelwe ukuhlaziya imitapo yolwazi ye-cDNA ehlelwe kumodi ye-Circular Consensus Sequencing (CCS) futhi ihlonze ukulandelana kwe-Full-Length Non-Chimeric (FLNC), okube sekuhlanganiswa kube yi-non-redundant transcripts. Ukuhlaziywa okulandelayo kwe-BUSCO kuhlola ukuphelela kwe-transcriptome assembly. Kusukela ku-transcriptome ehlanganisiwe, kwenziwa ukuhlaziywa okuningi: i-splicing ehlukile, i-simple sequence repeat (SSR), ukubikezela i-lncRNA kanye nezakhi zofuzo eziqondiwe ezihambisanayo, ukubikezela izakhi zofuzo ezintsha, ukuhlaziywa komndeni wezakhi zofuzo, ukuhlaziywa kwezinto zokubhala, kanye nokuchazwa okusebenzayo kwemibhalo.