Imikhiqizo

Ukulandelana Kohlobo Lonke Lwezitshalo/Izilwane

Ukulandelanisa Kohlobo Lonke Kwesitshalo/Isilwane Isithombe Esifakiwe

Ukulandelana kabusha kwe-genome okuphelele, okubuye kwaziwe nge-WGS, kuvumela ukudalulwa kwakho kokubili ukuguqulwa okuvamile nokungavamile kuyo yonke i-genome okuhlanganisa i-Single Nucleotide Polymorphism (SNP), Ukususwa Kokufaka (InDel), Ukuhluka Kwesakhiwo (SV), kanye Nokuhlukahluka Kwenombolo Kopisha (CNV ).Ama-SV enza ingxenye enkulu yesisekelo sokuhlukahluka kune-SNP futhi anomthelela omkhulu ku-genome, enomthelela obalulekile ezintweni eziphilayo.Ukuphinda kufundwe isikhathi eside kuvumela ukuhlonza okunembe kakhudlwana kwezingcezu ezinkulu nokuhluka okuyinkimbinkimbi ngoba ukufunda isikhathi eside kwenza kube lula kakhulu ukweqa i-chromosomal ezindaweni eziyinkimbinkimbi njengokuphindaphinda kwe-tandem, izifunda ezicebile ze-GC/AT, nezifunda eziguquguquka kakhulu.

Ipulatifomu: Illumina, PacBio, Nanopore

Xhumana nathi

Imininingwane Yesevisi

Umphumela wedemo

Ukushicilelwa Okufakiwe

1.Izinzuzo Zesevisi

Umuzwa obanzi wokulandelana kofuzo lwezinhlobo ezingaphezu kuka-1000.

Amacala ashicilelwe angaphezu kuka-800 anesici esinqwabelene esingaphezu kuka-4000.

Ukuhlaziywa kwe-bioinformatics okuphelele ekushayeni okuhlukile nokuhlaziywa komsebenzi.

2. Imininingwane Yesevisi

Inkundla		Umtapowolwazi	Ukujula kwe-seq okunconyiwe
Illumina		PE150	Nge-SNP, i-InDel ishayela ≥ 10x Ku-SV, i-CNY ishayela ≥ 30x
I-Nanopore		8 kb	Ku-SV, i-CNY ishayela ≥ 20x
Pacbio	I-CCS	15 kb	Nge-SNP, InDel, SV, CNY eshayayo ≥ 10x

3. Izidingo Zesampula

Inkundla		Conc.(ng/μL)	Inani (ng)	Ubumsulwa		Ijeli ye-agarose
				OD260/280	OD260/230	1. Sula ibhendi eyinhloko ngaphandle noma okulinganiselweukuwohloka okubonwa kujeli. 2. Cha noma i-RNA elinganiselwe noma ukungcoliswa kwamaprotheni
Illumina		≥1	≥30	-	-
I-Nanopore		≥30	Kuya ngesivuno sedatha 10μg/iseli	1.7-2.2	≥1.5
Pacbio	I-CCS	≥50		1.7-2.2	1.8-2.5

4. Ukuhlaziywa kwe-Bioinformation

5. Ukugeleza Komsebenzi Wenkonzo

Ukulethwa kwesampula

Ukukhishwa kwe-DNA

Ukwakhiwa komtapo wolwazi

Ukulandelanisa

Ukuhlaziywa kwedatha

Ukulethwa kwedatha

Okwedlule: I-BMKMANU S1000 Spatial Transcriptome

Olandelayo: I-Single-nucleus RNA Sequencing

1) Izibalo zeGenome Mapping

Ithebula 1 Izibalo zomphumela wemephu

Umfanekiso 1 Ukusatshalaliswa kosayizi wokufaka kanye nokuhlanganisa okufundekayo.

2) Shintsha Ukutholwa

Umfanekiso 2 Izibalo kanye nesichasiselo se-SNP/INDEL/SV phakathi kwamasampuli

Umfanekiso 3 Ukusatshalaliswa okubanzi kweGenome kwama-vatations

3) Isichasiselo esisebenzayo sokuhlukahluka

2019	Ukuxhumana kwemvelo	Ukulandelanisa kabusha kwegenome yonke kuveza imvelaphi ye-Brassica napus kanye ne-genetic loci ehilelekile ekuthuthukisweni kwayo
2020	I-PNAS	Umsuka wokuziphendukela kwemvelo kanye nomlando wokufuywa kwe-goldfish (Carassius auratus)
2021	Plant Biotechnology Journal	Izinkomba zofuzo zezinhlobo ezimbili ze-jute ezitshaliwe
2021	Plant Biotechnology Journal	Amasignesha e-Genomic wemifino nembewu ye-allopolyploid Brassicajuncea kanye ne-genetic loci elawula ukuqoqwa kwama-glucosinolates
2019	Isitshalo samangqamuzana	Ukuphindaphinda kabusha kwegenome kweqoqo lomhlaba wonke le-rapeseed accessions kuveza isisekelo sofuzo sokuhlukana kwabo kwe-ecotype
2022	Ucwaningo Lwezolimo	Ukuhlaziywa kwe-genome-wide association kunikeza ukuqonda kwamangqamuzana ekuhlukeni kwemvelo kosayizi wembewu yekhabe
2021	Ijenali ye-Experimental Botany	Ucwaningo lwe-Genome-wide association lukhomba okuhlukile kwe-GhSAD1 enikeza ukubekezelela okubandayo kukotini
2021	Ijenali ye-Experimental Botany	Ucwaningo lwe-Genome-wide association kanye nokuqhathaniswa kwe-transcriptome kuveza inoveli i-QTL kanye nezakhi zofuzo ezilawula usayizi we-petal ku-rapeseed.