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I-Chromatin Immunoprecipitation Sequencing (ChIP-seq)
I-ChIP-Seq inikeza iphrofayili ebanzi yofuzo lwezinjongo ze-DNA zokuguqulwa kwe-histone, izici zokuloba, namanye amaprotheni ahlobene ne-DNA.Ihlanganisa ukukhethwa kwe-chromatin immuno-precipitation (ChIP) ukuze kubuyiselwe ama-protein-DNA complexes athile, namandla okulandelana kwesizukulwane esilandelayo (NGS) okulandelana okuphezulu komphumela we-DNA etholiwe.Ukwengeza, ngenxa yokuthi ama-protein-DNA complexes atholakala kumangqamuzana aphilayo, izingosi ezibophayo zingaqhathaniswa ngezinhlobo zamangqamuzana nezicubu ezihlukene, noma ngaphansi kwezimo ezihlukene.Izicelo zisukela ekulawuleni okulotshiweyo kuye ezindleleni zokuthuthuka eziya ezindleleni zezifo nangale kwalokho.
Ipulatifomu: I-Illumina NovaSeq Platform
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Ukulandelana okuphelele kwe-genome bisulfite
I-DNA methylation endaweni yesihlanu ku-cytosine (5-mC) inethonya elibalulekile ekukhulumeni kwezakhi zofuzo kanye nomsebenzi wamaselula.Amaphethini e-methylation angavamile ahlotshaniswa nezimo nezifo eziningana, njengomdlavuza.I-WGBS isiphenduke indinganiso yegolide yokufunda i-genome-wide methylation ngokulungiswa kwesisekelo esisodwa.
Ipulatifomu: I-Illumina NovaSeq Platform
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I-Assay ye-Chromatin Efinyelelekayo Ye-Transposase Enokulandelanisa Okuphezulu Kokudlula (i-ATAC-seq)
I-ATAC-seq iyindlela yokulandelanisa ephezulu yokuhlaziya ukufinyeleleka kwe-chromatin yohlobo lonke, okubalulekile ekulawuleni i-epigenetic yomhlaba wonke ekukhulumeni kofuzo.Ama-adaptha alandelanayo afakwa ezifundeni ze-chromatin nge-hyperactive Tn5 transposase.Ngemuva kokukhulisa i-PCR, kwakhiwa umtapo wolwazi wokulandelanisa.Zonke izifunda ze-chromatin ezivulekile zingatholwa ngaphansi kwesimo esithile sesikhathi sesikhala, hhayi kuphela kumasayithi abophezelayo we-transcription factor, noma isifunda esithile esilungisiwe se-histone.
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I-Reduced Representation Bisulfite Sequencing (RRBS)
Ucwaningo lwe-DNA methylation belulokhu luyisihloko esishisayo ocwaningweni lwezifo, futhi luhlobene kakhulu nezakhi zofuzo kanye nezici ze-pheno-typic.I-RRBS iyindlela enembile, ephumelelayo neyongayo yocwaningo lwe-DNA methylation.Ukunothiswa kwezifunda eziqhingini zomgqugquzeli kanye ne-CpG nge-enzymatic cleavage (Msp I), kuhlanganiswe nokulandelana kwe-Bisulfite, kunikeza ukutholwa kwe-DNA methylation yokuxazulula okuphezulu.
Ipulatifomu: I-Illumina NovaSeq Platform
