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Amalabhulali enziwe kusengaphambili e-DNBSEQ
I-DNBSEQ, eyathuthukiswa yi-MGI, ubuchwepheshe obusha be-NGS obukwazile ukunciphisa izindleko zokulandelana nokwandisa umthamo. Ukulungiswa kwemitapo yolwazi ye-DNBSEQ kuhilela ukuhlukaniswa kwe-DNA, ukulungiswa kwe-ssDNA, kanye nokukhulisa i-rolling circle ukuthola ama-nanoball e-DNA (DNB). Lawa abese elayishwa endaweni eqinile bese elandelaniswa yi-combinatorial Probe-Anchor Synthesis (cPAS). Ubuchwepheshe be-DNBSEQ buhlanganisa izinzuzo zokuba nesilinganiso samaphutha esiphansi sokukhulisa nokusebenzisa amaphethini amaphutha aphezulu ngama-nanoball, okuholela ekulandeleni umthamo ophezulu kanye nokunemba.
Insizakalo yethu yokulandelelanisa amalabhulali eyenziwe ngaphambilini ivumela amakhasimende ukuthi alungiselele amalabhulali okulandelelanisa ama-Illumina avela emithonjeni eyahlukahlukene (i-mRNA, i-genome ephelele, i-amplicon, amalabhulali angu-10x, phakathi kwabanye), aguqulwa abe amalabhulali e-MGI emalabhorethri ethu ukuze ahlelwe ngokulandelana ku-DNBSEQ-T7, okuvumela inani eliphezulu ledatha ngezindleko eziphansi.
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Imitapo yolwazi eyenziwe kusengaphambili ye-Illumina
Ubuchwepheshe bokulandelana kwe-Illumina, obusekelwe ku-Sequencing by Synthesis (SBS), buyindlela entsha ye-NGS eyamukelwa emhlabeni wonke, ephethe ukukhiqiza idatha yokulandelana engaphezu kuka-90% emhlabeni. Isimiso se-SBS sihilela ukuthwebula izithombe ze-terminator eziguquguqukayo ezibhalwe nge-fluorescent njengoba i-dNTP ngayinye ingezwa, bese ihlukaniswa ukuze kuvunyelwe ukufakwa kwesisekelo esilandelayo. Njengoba wonke ama-dNTP amane aguquguqukayo aboshwe yi-terminator akhona kumjikelezo ngamunye wokulandelana, ukuncintisana kwemvelo kunciphisa ukubandlulula kokufakwa. Lobu buchwepheshe obuguquguqukayo busekela kokubili imitapo yolwazi efundwayo kanye neyamabili, bunakekela izinhlelo zokusebenza ezahlukahlukene ze-genomic. Amakhono aphezulu okusebenzisa i-Illumina sequencing kanye nokunemba kuyibeka njengetshe lesisekelo ocwaningweni lwe-genomics, okunika amandla ososayensi ukuthi bahlukanise ubunzima be-genomes ngemininingwane engenakuqhathaniswa kanye nokusebenza kahle.
Insizakalo yethu yokulandelelanisa amalabhulali eyenziwe ngaphambilini ivumela amakhasimende ukuthi alungiselele amalabhulali okulandelelanisa amalabhulali avela emithonjeni eyahlukahlukene (i-mRNA, i-genome ephelele, i-amplicon, amalabhulali ayi-10x, phakathi kwabanye). Ngemva kwalokho, lawa malabhulali angathunyelwa ezikhungweni zethu zokulandelelanisa amalabhulali ukuze kulawulwe ikhwalithi kanye nokulandelelanisa amapulatifomu e-Illumina.
