Imikhiqizo

Ukulandelana kwe-Prokaryotic RNA

Ukulandelana kwe-Prokaryotic RNA kusebenzisa ukulandelana kwesizukulwane esilandelayo (NGS) ukuveza ubukhona nenani le-RNA ngesikhathi esithile, ngokuhlaziya i-transcriptome yeselula eshintshayo.Ukulandelana kwe-RNA ye-prokaryotic yenkampani yethu, ihloselwe ngokukhethekile ama-prokaryote anama-genome ayireferensi, ikunikeza ngephrofayela ye-transcriptome, ukuhlaziywa kwesakhiwo sofuzo, njll. Isetshenziswe kabanzi ocwaningweni lwesayensi oluyisisekelo, ucwaningo lwezidakamizwa kanye nokuthuthukiswa, nokunye.

Ipulatifomu: I-Illumina NovaSeq Platform

Xhumana nathi

Imininingwane Yesevisi

Ipulatifomu: Iplatifomu ye-Illumina NovaSeq, PE150

Uhlobo lwelabhulali: 350bp faka umtapo wezincwadi we-cDNA (kuncike ekusabalaliseni okuphezulu)

Isu lokulandelanisa: Okubhangqiwe-Isiphetho esingu-150 bp

Ncoma inani ledatha: 2 Gb idatha/isampula eluhlaza

I-Bioinformatics

(1) Ukulandelanisa ukulawulwa kwekhwalithi yedatha: Ukusatshalaliswa kwe-Nucleotide, ukusatshalaliswa kwekhwalithi eyisisekelo, ukuhlolwa kwesilinganiso se-rRNA;

(2) Ukuhlaziywa kokulandelana kokulandelana: Izibalo zomphumela wokuqondanisa, Ukugcwaliswa kwesikhala kokulandelana, Ukufakwa kokulandelana;

(3) Isichasiselo se-genome esibhekisela (NR, Swiss-Prot, Pfam, COG, GO, KEGG);

(4) Ukuhlaziywa kokuvezwa: Ukusatshalaliswa kokuveza (Ngamasampuli amabili noma ngaphezulu), Ukuhlaziywa kwenkulumo phakathi kwamasampuli (ukuhlaziywa kwe-Venn, ukuhlaziywa kwe-Correlation, ukuhlaziywa kwe-PCA);

(5) Ukuhlaziya isisho esihlukile (Ngamasampuli amabili noma ngaphezulu);

(6) Ukuhlaziywa kwesethi yofuzo: Ukuhlaziywa kwe-Venn, ukuhlaziywa kwe-Cluster, ukuhlaziywa kwe-Function annotation (COG, GO, KEGG), Ukuhlaziywa kokucebisa umsebenzi (GO, KEGG), igrafu yokucebisa umsebenzi, ukuhlaziywa kwe-Ipath;

(7) Ukuhlaziywa kwe-sRNA: ukubikezela kwe-sRNA, isichasiselo se-sRNA (Blast, sRNAMAp, sRNATarBase, SIPHT, Rfam), ukubikezela kwesakhiwo sesibili se-sRNA, ukubikezela kofuzo okuhlosiwe kwe-sRNA;

(8) Ukuhlaziywa kwesakhiwo sombhalo obhaliwe: Ukuhlaziywa kwe-Operon, ukubikezela kwezindawo zokuqala neziphetho zokulotshwa, isichasiselo se-UTR nokuhlaziya, Ukubikezela kokulandelana komthuthukisi, ukuhlaziywa kwe-SNP/InDel (isichasiselo se-SNP/InDel, ukusatshalaliswa kwesifunda kwe-SNP/InDel, izibalo ze-SNP).