Ukulandelelana Kwe-RNA Encane-Illumina
Izici
● Ukulungiselela umtapo wolwazi wokukhetha usayizi.
● Ukuhlaziywa kwe-Bioinformatic kugxile ekubikezelweni kwe-miRNA kanye nezinhloso.
Izinzuzo Zesevisi
●Ubuchwepheshe Obubanzi: Sicubungule amasampula angaphezu kuka-300, ahlanganisa izinhlobo ezahlukene zamasampula. Siletha ulwazi oluningi kuyo yonke iphrojekthi.
●Ukulawulwa Kwekhwalithi Okuqinile: Sisebenzisa amaphuzu okulawula ayisisekelo kuzo zonke izigaba, kusukela ekulungiseleleni amasampula kuya ekulungiseleleni umtapo wolwazi, ukulandelana kanye ne-bioinformatics. Ukuqapha kwethu ngokucophelela kuqinisekisa ukulethwa kwemiphumela esezingeni eliphezulu njalo.
●Ukuhlaziywa okuphelele kwe-bioinformatics:Ivumela ukuhlonza ama-miRNA aziwayo nawakamuva, ukuhlonza izinhloso zama-miRNA, kanye nokuchazwa okusebenzayo okuhambisanayo kanye nokucebisa ngemininingwane eminingi (i-KEGG, GO).
●Usekelo Lwangemva Kokuthengisa: Siyaqonda ukuthi kubaluleke kangakanani ukuba khona, yingakho ukuzibophezela kwethu kudlulela ngale kokuphothulwa kwephrojekthi ngesikhathi senkonzo yezinyanga ezintathu ngemuva kokuthengisa. Phakathi nalesi sikhathi, sinikeza ukulandelwa kwephrojekthi, usizo lokuxazulula izinkinga, kanye nezikhathi ze-Q&A ukuze kuxazululwe noma yimiphi imibuzo ehlobene nemiphumela.
Izidingo Zesampula Nokulethwa
| Umtapo Wolwazi | Ipulatifomu | Idatha enconyiwe | Idatha ye-QC |
| Usayizi ukhethiwe | I-Illumina SE50 | Ukufundwa okungu-10M-20M | Q30≥85% |
Izidingo Zesampula:
Ama-Nucleotide:
| I-Conc.(ng/μl) | Inani (μg) | Ubumsulwa | Ubuqotho |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Ukungcoliswa kwephrotheni noma i-DNA okulinganiselwe noma okungekho nhlobo kukhonjisiwe kujeli. | I-RIN≥6.0; 5.0≥28S/18S≥1.0; ukuphakama okulinganiselwe noma okungekho kwesisekelo |
● Izitshalo:
Impande, Isiqu noma Ipetali: 450 mg
Iqabunga noma Imbewu: 300 mg
Izithelo: 1.2 g
● Isilwane:
Inhliziyo noma Amathumbu: 450 mg
I-Viscera noma Ubuchopho: 240 mg
Imisipha: 600 mg
Amathambo, Izinwele noma Isikhumba: 1.5g
● Ama-arthropod:
Izinambuzane: 9g
I-Crustacea: 450 mg
● Igazi eliphelele: Amapayipi ama-2
● Amaseli: 106 amaseli
● I-Serum ne-Plasma:6 mL
Ukulethwa Kwesampula Okunconyiwe
Isitsha: Ishubhu le-centrifuge elingu-2 ml (Akukhuthazwa ukusebenzisa i-tin foil)
Ukulebula kwesampula: Iqembu + ukukopisha isib. A1, A2, A3; B1, B2, B3.
Ukuthunyelwa:
1. Iqhwa elomile: Amasampula kudingeka apakishwe ezikhwameni bese egqitshwa eqhweni elomile.
2. Amashubhu e-RNAstable: Amasampula e-RNA angomiswa eshubhu lokuzinzisa i-RNA (isb. i-RNAstable®) bese ethunyelwa ekushiseni kwegumbi.
Ukugeleza Komsebenzi Wesevisi
Umklamo wokuhlola
Ukulethwa kwesampula
Ukukhishwa kwe-RNA
Ukwakhiwa komtapo wolwazi
Ukulandelana
Ukuhlaziywa kwedatha
Izinsizakalo zangemuva kokuthengisa
I-Bioinformatics
● Ukulawulwa kwekhwalithi yedatha eluhlaza
● Ukuhlukaniswa kwe-RNA encane
● ukuhlaziywa kokubonakaliswa kwe-miRNA kanye nokwehluka kokubonakaliswa
● i-gene target ye-miRNA kanye ne-gene target ye-miRNA evezwe ngokuhlukile
● Isichasiselo sezakhi zofuzo eziqondiwe kanye nokucebisa umsebenzi wezakhi zofuzo ze-miRNA ezivezwe ngokuhlukile
Ukuhlonza i-miRNA: isakhiwo kanye nokujula
Ukuvezwa okuhlukile kwe-miRNA - ukuhlanganiswa kwe-hiearchical
Isichasiselo esisebenzayo senhloso yama-miRNA achazwe ngokuhlukile
Hlola intuthuko yocwaningo eyenziwe yizinsizakalo zokulandelana kwe-sRNA ze-BMKGene ngokusebenzisa iqoqo lezincwadi ezikhethiwe.
UChen, H. et al. (2023) 'Ukutheleleka ngegciwane kuvimbela ukwenziwa kwe-saponin kanye ne-photosynthesis ku-Panax notoginseng',I-Physiology Yezitshalo kanye ne-Biochemistry, 203, ikhasi 108038. doi: 10.1016/J.PLAPHY.2023.108038.
ULi, H. et al. (2023) 'I-FYVE domain equkethe iphrotheni i-FREE1 ihlotshaniswa nezingxenye ze-microprocessor ukuze kuvinjelwe i-miRNA biogenesis',Imibiko ye-EMBO, 24(1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. et al. (2023) 'I-MicroRNA Ame-Bantam-3p Ilawula Ukuthuthukiswa Kwezimpungushe Zezibungu Ngokuqondisa I-Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) ku-Honeybee, Apis mellifera',Ijenali Yomhlaba Wonke Yesayensi Yamamolekyuli, 24(6), ikhasi 5726. doi: 10.3390/IJMS24065726/S1.
UZhang, M. et al. (2018) 'Ukuhlaziywa Okuhlanganisiwe kwe-MiRNA kanye ne-Gene Okuhlobene Nekhwalithi Yenyama Kwembula ukuthi i-Gga-MiR-140-5p Ithinta Ukufakwa Kwamafutha Emisipheni Ezinkukhu',I-Cellular Physiology kanye ne-Biochemistry, 46(6), amakhasi 2421-2433. doi: 10.1159/000489649.
