Ukukhuthazwa Kwesikhathi Esilinganiselwe: Isixazululo Sokuhlanganiswa Kwe-T2T Genome Konke Ku-One
Ngokuthuthuka okusheshayo kobuchwepheshe bokulandelana kwezakhi zofuzo kanye ne-algorithm yokuhlanganisa, ukuhlanganiswa kwezakhi zofuzo ze-Telomere-to-Telomere (T2T) sekuyindlela ejwayelekile yocwaningo lwezakhi zofuzo olusezingeni eliphezulu, ukuxazulula inkinga yegebe ende ekuhlanganisweni kwezakhi zofuzo zendabuko (Izifunda ezinokuphindaphinda okuphezulu kanye nokuqukethwe okuphezulu kwe-GC, njenge-telomeres kanye ne-centromeres, zidinga idatha yokulandelana ende kakhulu ukuze zifinyelele ngempumelelo). Ukuze sikhuthaze ukuthandwa kobuchwepheshe be-T2T ocwaningweni lwezemfundo, sithole ukulandelana kwezakhi zofuzo okuphelele, futhi sivumele ukutholakala kwezakhi zofuzo ezazingaziwa ngaphambili kanye nokwehluka kwesakhiwo, sethula lokhu kukhushulwa kwesikhathi esilinganiselwe, sinikeza isisombululo sokuhlanganiswa kwezakhi zofuzo ze-T2T esisodwa esingabizi kakhulu sezinhlobo zakho, sinike amandla ucwaningo lwakho ngereferensi ephelele nenembile.
Imininingwane Yephakheji Yesevisi
●Ukulandelana
● Ukufundwa okufushane kwe-Illumina kwe-Survey: 50X
● I-PacBio HiFi ifundeka ukuze kuhlanganiswe i-draft: 50X
● I-Hi-C yokubeka isikhafu: 100X
● Ama-Nanopore amade kakhulu okufunda ukugcwalisa izikhala: amaseli ama-2
● I-Illumina mRNA-seq yesichasiselo se-genome: 12 Gb / 1 pool
●Ukuhlaziywa Kwezinga Lokulandelana Kwezikhundla
● Ucwaningo lokuhlolwa kwesici se-genome
● Ukuhlanganiswa kwe-genome yezinga le-contig
● I-scaffolding ye-genome yezinga le-chromosome
● Ukugcwaliswa kwezikhala kanye nokuhlonza i-telomere ye-genome ye-T2T
● Ukuhlolwa kwekhwalithi ye-genome ye-T2T
● Isichasiselo se-genome sokubikezela izakhi ze-gene kanye ne-genomics
● Umbiko wokuhlaziya ophelele onencazelo yobuchwepheshe yobungcweti
Umphumela Wedemo
Ukuze siqonde kangcono ikhwalithi yesevisi yethu yokuhlanganisa i-genome ye-T2T, sinikeza umphumela wokubonisa wephrojekthi ejwayelekile:
•I-genome yokugcina ye-T2T inama-chromosome angu-19 anobude obungu-491.39Mb, kanye ne-N50 ehlanganisiwe engu-26.04Mb. Kwatholakala ama-telomere angu-38 nama-centromere angu-19. Ama-chromosome angu-19 angama-chromosome angenazikhala phakathi kwe-telomere ne-telomere.
•Imiphumela yokuhlola ye-Assembly ikhombisa ukuphelela okuphezulu kanye nokunemba kwe-genome ehlanganisiwe: Ukuhlolwa kwe-BUSCO kusetshenziswa isizindalwazi se-embryophyta se-OrthoDB 10 kubonise ukuphelela kwe-genome okungu-97.09%; Inani le-QV ye-genome lingu-52.36. Inani le-LAI ye-genome lingu-18.93.
Ukusebenza
•Izinkambu zocwaningo ezisebenzayo: Ucwaningo oluyisisekelo lwe-genomic, ukuthuthukiswa kwezakhi zofuzo zezitshalo, ukuzalaniswa kwezilwane, zonke izinsimu zezitshalo nezilwane ezidinga ukuhlanganiswa kwezakhi zofuzo ezisezingeni eliphezulu.
•Izinhlobo ezisebenzayo: Usayizi we-genome ≤ 1; Izilwane ezincelisayo, izitshalo ezivamile, izithelo ezivamile nama-melon, izihlahla zasehlathini ezivamile, izinhlanzi zasemanzini ahlanzekile, njll. Ukuze uthole ezinye izinhlobo, sicela uthintane ukuze uthole imininingwane.
•Ukukhushulwa kusebenza: Izinkontileka Zisayinwe ngaphambi kukaJuni 30.
•Imiyalelo eyengeziwe: Kumasampula akhethekile (isb., lawo anezilandelaniso eziphindaphindwayo eziphezulu noma ikhwalithi ephansi ye-DNA) noma izidingo zokuhlaziya ezenziwe ngokwezifiso, ithimba lethu lobuchwepheshe lizohlinzeka ngesisombululo esenziwe ngokwezifiso, ngentengo elungisiwe ngokusekelwe ezidingweni zangempela zephrojekthi.
Qala Manje
Sebenzisa leli thuba lesikhathi esilinganiselwe futhi ujabulele isevisi yokuhlanganisa i-genome ye-T2T esezingeni eliphezulu ngentengo ekhethwayo! Xhumana nethimba lethu lobuchwepheshe ukuze ubheke imininingwane, uthumele ulwazi lwesampula, futhi uthole isilinganiso esenziwe ngokwezifiso.
