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Imikhiqizo

Ikhithi Yokuhlanza I-DNA Ehlakaniphile ye-TGuide

Isithombe Esifakiwe Sekhithi Yokuhlanza I-TGuide Smart DNA

Ithola i-DNA esezingeni eliphezulu kumkhiqizo we-PCR noma amajeli e-agarose.

Xhumana nathi

Imininingwane Yesevisi

Inombolo yekhathalogi / ukupakishwa

Cha ikati	ID	Inani lamalungiselelo
4993550 (ikhatriji)	I-DP642-DE	48

Umhlahlandlela wokukhetha ama-reagent we-TGuide S16

I-Nuclei Acid	Uhlobo Lwesampula	Igama Lomkhiqizo	Usayizi wokupakisha (okulungiswayo) Ikhatriji/ ipuleti	Inombolo yekati. Ikhatriji/ipuleti
I-DNA	Izicubu Zezilwane	I-TGuide Smart Magnetic Tissue DNA Kit	48/96	4993547/4995038
I-DNA	Isitshalo Nembewu	I-TGuide Smart Magnetic Plant DNA Kit	48	4993548
I-DNA	Umhlabathi/Isihlalo	I-TGuide Smart Soil/Stool DNA Kit	48	4993549
I-DNA	Imikhiqizo yejeli ne-PCR	Ikhithi Yokuhlanza I-DNA Ehlakaniphile ye-TGuide	48	4993550
I-DNA	Igazi Eliphelele	I-TGuide Smart Blood Genomic DNA Kit	48/96	4993703/4995206
I-DNA	Amaseli/I-Swab/Amabala Omile, njll.	I-TGuide Smart Universal DNA Kit	48/96	4993704/4995040
I-RNA	Igazi/Iseli/Izicubu	I-TGuide Smart Blood/Cell/Tissue RNA Kit	48/96	4993551/4995043
I-RNA	Isitshalo Nembewu	I-TGuide Smart Magnetic Plant RNA Kit	48	4993552
I-DNA/i-RNA	Ama-Fluids Angenamaseli (i-Serum, njll.)	I-TGuide Smart Viral DNA/RNA Kit	48/96	4993702/4995207

Isingeniso somkhiqizo

Ithola i-DNA ngokushesha nangendlela efanele kusuka kujeli ye-TAE/TBE kanye nomkhiqizo we-PCR.

Ububanzi bosayizi we-DNA fragment: 100 bp-15 kb.

I-DNA etholakale ngokusebenzisa le khithi ingasetshenziselwa ukugaya ukudla, i-PCR, ukulandelana, ukuhlolwa komtapo wolwazi, ukubopha, ukuguqulwa kanye nokunye ukuhlolwa.

Izici

Kulula kakhulu ukuyisebenzisa

Akukho msebenzi owengeziwe wokufaka amapayipi.

Ivolumu yokulungisa ingalungiswa njengoba kudingeka.

Ukufakwa nokusebenza akudingi ukuqeqeshwa okuningi. Ngezinhlelo ezifakwe ngaphambilini, vula ikhatriji, khetha iphrothokholi bese usebenzisa ukuhlolwa kwakho. Izinhlelo zilungele ukusetshenziswa, futhi zingenziwa ngezifiso.

Ama-reagent alayishiwe kusengaphambili kanye nezinto ezisetshenziswayo ezilahlwayo ezifanayo.

Ukuphathwa kwamakhemikhali kuyasuswa ngezinga elikhulu ukuqinisekisa ukuphepha kokusebenza.

Umphumela Wedemo

Ukuhlanzwa nokubuyiselwa kwe-DNA ye-300 bp segment agarose gel
Ukuhlushwa kwejeli ye-Agarose:1.5%(TBE)
Iyalayisha
ivolumu: 8 μl
Umaka: 50 bp isitebhisi se-DNA, i-TIANGEN
I-S16-01, i-S16-02, kanye ne-S16-03 zimelela ukuhlolwa kokuhlanza nokubuyisa isampula okuhambisanayo kusetshenziswa ama-3 TGuide S16 Nucleic Acid Extractors ane-TGuide Smart DNA Purification Kit (4993550) ngesikhathi esisodwa.
Inkampani: ikhithi yokukhipha ikholomu ejikelezayo ngesandla yomkhiqizo odumile

Ukuhlanzwa nokubuyiselwa kwe-DNA kwe-2000 bp segment agarose gel
Ukuhlushwa kwejeli ye-Agarose:1.5% (TBE)
Ivolumu yokulayisha: 8 μl
Umaka: D2000, TIANGEN
I-S16-01, i-S16-02, kanye ne-S16-03 zimelela ukuhlanzwa nokubuyiselwa kwesampula okufanayo
zama ukusebenzisa ama-TGuide S16 Nucleic Acid Extractors amathathu ane-TGuide Smart DNA Purification Kit (4993550) ngesikhathi esisodwa.
Inkampani: ikhithi yokukhipha ikholomu ejikelezayo ngesandla yomkhiqizo odumile

Umphumela wokuhlola: ukusebenzisa i-TGuide S16 Nucleic Acid Extractor enekhithi ekhethekile i-TGuide Smart DNA Purification Kit (4993550) kungafeza ukuzenzekela okuphelele kokululama kwejeli. Ukuhambisana kokuhlanzwa nokululama kuhle kakhulu, futhi umphumela wokukhipha ungcono kunowemikhiqizo encintisanayo yezinye izinkampani.

Ukubuyiswa kwe-DNA ye-15000 bp section agarose gel kanye nokuhlanzwa komkhiqizo we-PCR
Ukuhlushwa kwejeli ye-Agarose :1% (TBE)
Ivolumu yokulayisha: 3 μl
Umaka:
D15000,TIANGEN
G1, G2: ukululama kwejeli ye-agarose
C1: Ukuhlanzwa komkhiqizo we-PCR.
Iphrothokholi esetshenziswa ngesandla: ikhithi yokukhipha ikholomu ejikelezayo ngesandla yomkhiqizo odumile u-B
Inkampani: ikhithi yokukhipha esekwe endleleni ye-magnetic bead yomkhiqizo owaziwayo

Umphumela wokuhlola: kusetshenziswa i-TGuide S16 Nucleic Acid Extractor enekhithi i-TGuide Smart DNA Purification Kit (4993550), ukubuyiselwa kwejeli le-agarose kanye nokuhlanzwa komkhiqizo we-PCR kungatholakala ngokuzenzakalelayo, futhi umphumela wokuhlanza ufana nalowo wokuhlanza okusekelwe ku-spin-column.