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I-Transcriptomics

  • Full-length mRNA sequencing-Nanopore

    Ukulandelana kwe-mRNA yobude obugcwele-Nanopore

    Ukulandelana kwe-RNA kube yithuluzi eliyigugu lokuhlaziya okuphelele kwe-transcriptome.Ngokungangabazeki, ukulandelana kwendabuko kokufunda okufushane kuzuze intuthuko eminingi ebalulekile lapha.Noma kunjalo, imvamisa ihlangabezana nemikhawulo ekukhombeni kobude obugcwele be-isoform, ukulinganisa, ukuchema kwe-PCR.

    Ukulandelana kwe-Nanopore kuzihlukanisa kwezinye izinkundla zokulandelana, ngokuthi ama-nucleotide afundwa ngokuqondile ngaphandle kokuhlanganiswa kwe-DNA futhi akhiqize ukufunda isikhathi eside emashumini ama-kilobase.Lokhu kunikeza amandla okulotshiweyo okufundwayo okuqondile okuwela ubude obugcwele kanye nokubhekana nezinselele ezifundweni zeleveli ye-isoform.

    Inkundla:I-Nanopore PromethION

    Umtapowolwazi:cDNA-PCR

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  • De novo Full-length Transcriptome sequencing -PacBio

    I-Novo Ukulandelana kwe-Transcriptome yobude obugcwele -PacBio

    De novoukulandelana kwe-transcriptome yobude obugcwele, eyaziwa nangokuthiDe novoI-Iso-Seq ithatha izinzuzo ze-sequencer ye-PacBio ngobude bokufunda, okuvumela ukulandelana kwama-molecule e-cDNA anobude obugcwele ngaphandle kokuphuka.Lokhu kugwema ngokuphelele noma imaphi amaphutha akhiqizwe ezinyathelweni zokuhlanganisa okulotshiweyo futhi kwakha amasethi e-unigene anokulungiswa kwezinga le-isoform.Lawa masethi e-unigene ahlinzeka ngolwazi olunamandla lofuzo njengokuthi "i-reference genome" ezingeni le-transcriptome.Ukwengeza, ukuhlanganisa nedatha yokulandelana kwesizukulwane esilandelayo, le sevisi inika amandla ukulinganisa okunembile kwenkulumo yezinga le-isoform.

    I-Platform: I-PacBio Sequel II
    Umtapo wolwazi: Umtapo wensimbi we-SMRT
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  • Eukaryotic mRNA sequencing-Illumina

    I-Eukaryotic mRNA ukulandelana-Illumina

    Ukulandelana kwe-mRNA kunika amandla ukuphrofayela wawo wonke ama-mRNA alotshwe kusuka kumaseli ngaphansi kwezimo ezithile.Ubuchwephesha obunamandla bokuveza iphrofayili yesisho sofuzo, izakhiwo zofuzo nezindlela zamangqamuzana ezinqubo ezithile zebhayoloji.Kuze kube manje, ukulandelana kwe-mRNA sekusetshenziswe kabanzi ocwaningweni oluyisisekelo, ekuxilongweni komtholampilo, ekuthuthukisweni kwezidakamizwa, njll.

    Ipulatifomu: Illumina NovaSeq 6000

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  • Non-Reference based mRNA sequencing-Illumina

    I-Non-Reference based mRNA sequencing-Illumina

    Ukulandelana kwe-mRNA kusebenzisa inqubo yesizukulwane esilandelayo sokulandelanisa (NGS) ukuze ithwebule i-RNA(mRNA) yesithunywa ifomu i-Eukaryote ngesikhathi esithile imisebenzi ethile ekhethekile eyenza isebenze.Okulotshiweyo okude kakhulu okuhlukanisiwe kwakubizwa ngokuthi 'i-Unigene' futhi kwasetshenziswa njengochungechunge lwereferensi ukuze kuhlaziywe okwalandela, okuyindlela ephumelelayo yokufunda indlela yamangqamuzana nenethiwekhi yokulawula yohlobo ngaphandle kwereferensi.

    Ngemuva kokuhlanganiswa kwedatha ye-transcriptome kanye nesichasiselo esisebenzayo se-unigene

    (1) Ukuhlaziywa kwe-SNP, ukuhlaziywa kwe-SSR, ukubikezela kwe-CDS kanye nesakhiwo sofuzo kuzokwenziwa kusengaphambili.

    (2) Ukulinganisa kwenkulumo ye-unigene kusampula ngayinye kuzokwenziwa.

    (3) Ama-unigene avezwe ngokuhlukile phakathi kwamasampuli (noma amaqembu) azotholwa ngokusekelwe ekukhulumeni okungafani

    (4) Ukuhlanganisa, isichasiselo esisebenzayo kanye nokuhlaziywa kokunothisa kwama-unigene avezwe ngokwehlukana kuzokwenziwa.

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  • Long non-coding sequencing-Illumina

    Ukulandelana okude okungafaki amakhodi-Illumina

    Ama-RNA amade angafaki amakhodi (lncRNAs) awuhlobo lwama-molecule e-RNA anobude obudlula ama-200 nt, abonakala ngamandla aphansi kakhulu okubhala amakhodi.I-LncRNA, njengelungu eliyinhloko kuma-RNA angafaki amakhodi, itholakala kakhulu ku-nucleus neplasma.Ukuthuthukiswa kobuchwepheshe bokulandelana kanye ne-bioinformatics kwenza ukukhonjwa kwamanoveli amaningi e-lncRNA futhi kuhlotshaniswe nalawo anemisebenzi yebhayoloji.Ubufakazi obunqwabelene buphakamisa ukuthi i-lncRNA ibandakanyeka kakhulu kumthethonqubo we-epigenetic, ukulawulwa kokulotshiweyo kanye nomthethonqubo wangemva kokubhala.

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  • Small RNA sequencing-Illumina

    I-RNA encane yokulandelanisa-Illumina

    I-RNA encane ibhekisela ekilasini lama-molecule e-RNA angafaki amakhodi ngokuvamile angaphansi kuka-200nt ngobude, okuhlanganisa i-RNA encane (miRNA), ukuphazamiseka okuncane kwe-RNA (siRNA), kanye ne-piwi-interacting RNA (piRNA).

    I-MicroRNA (miRNA) ikilasi le-RNA encane engokwemvelo enobude obungaba ngu-20-24nt, edlala izindima ezibalulekile zokulawula kumaseli.I-miRNA ibandakanyeka ezinqubweni eziningi zempilo eziveza izicubu - eziqondile kanye nesiteji - inkulumo ethize futhi ilondolozwe kakhulu ezinhlobonhlobo zezilwane.

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  • circRNA sequencing-Illumina

    i-circRNA sequencing-Illumina

    Ukulandelana okuphelele kwe-transcriptome kuklanyelwe ukuphrofayilila zonke izinhlobo zama-molecule e-RNA, okuhlanganisa ukufaka ikhodi (mRNA) nama-RNA angafaki amakhodi (okuhlanganisa i-lncRNA, i-circRNA ne-miRNA) alotshwa amaseli athile ngesikhathi esithile.Ukulandelana okuphelele kwe-transcriptome, okwaziwa nangokuthi "ingqikithi yokulandelana kwe-RNA" ihlose ukuveza amanethiwekhi okulawula aphelele ezingeni le-transcriptome.Ngokusizakala kobuchwepheshe be-NGS, ukulandelana kwayo yonke imikhiqizo ye-transcriptome kuyatholakala ukuze kuhlaziywe i-ceRNA kanye nokuhlaziya okuhlanganyelwe kwe-RNA, okunikeza isinyathelo sokuqala esibhekise ekulingiseni okusebenzayo.Iveza inethiwekhi yokulawula ye-ceRNA esekelwe ku-circRNA-miRNA-mRNA.

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  • Whole transcriptome sequencing – Illumina

    Ukulandelana okuphelele kwe-transcriptome - Illumina

    Ukulandelana okuphelele kwe-transcriptome kuklanyelwe ukuphrofayilila zonke izinhlobo zama-molecule e-RNA, okuhlanganisa ukufaka ikhodi (mRNA) nama-RNA angafaki amakhodi (okuhlanganisa i-lncRNA, i-circRNA ne-miRNA) alotshwa amaseli athile ngesikhathi esithile.Ukulandelana okuphelele kwe-transcriptome, okwaziwa nangokuthi "ingqikithi yokulandelana kwe-RNA" ihlose ukuveza amanethiwekhi okulawula aphelele ezingeni le-transcriptome.Ngokusizakala kobuchwepheshe be-NGS, ukulandelana kwayo yonke imikhiqizo ye-transcriptome kuyatholakala ukuze kuhlaziywe i-ceRNA kanye nokuhlaziya okuhlanganyelwe kwe-RNA, okunikeza isinyathelo sokuqala esibhekise ekulingiseni okusebenzayo.Iveza inethiwekhi yokulawula ye-ceRNA esekelwe ku-circRNA-miRNA-mRNA.

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  • Prokaryotic RNA sequencing

    Ukulandelana kwe-Prokaryotic RNA

    Ukulandelana kwe-Prokaryotic RNA kusebenzisa ukulandelana kwesizukulwane esilandelayo (NGS) ukuveza ubukhona nenani le-RNA ngesikhathi esithile, ngokuhlaziya i-transcriptome yeselula eshintshayo.Ukulandelana kwe-RNA ye-prokaryotic yenkampani yethu, ihlose ngokukhethekile kuma-prokaryote anama-genomes ayireferensi, ikunikeza iphrofayili ye-transcriptome, ukuhlaziywa kwesakhiwo sofuzo, njll. Isetshenziswe kabanzi ocwaningweni lwesayensi oluyisisekelo, ucwaningo lwezidakamizwa kanye nokuthuthukiswa, nokunye.

    Ipulatifomu: Illumina NovaSeq 6000

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  • Metatranscriptome Sequencing

    Ukulandelana kwe-Metatranscriptome

    Ukulandelana kwe-Metatranscriptome kukhomba ukubonakaliswa kofuzo kwamagciwane (kokubili i-eukaryote nama-prokaryote) endaweni yemvelo (okungukuthi, inhlabathi, amanzi, ulwandle, indle, namathumbu.). Ngokukhethekile, lezi zinsizakalo zikuvumela ukuthi uthole iphrofayili yesisho sofuzo esiphelele semiphakathi yamagciwane ayinkimbinkimbi, ukuhlaziywa kwe-taxonomic. kwezinhlobo zezilwane, ukuhlaziya okunothisayo kokusebenza kwezakhi zofuzo ezivezwe ngendlela ehlukile, nokunye.

    Ipulatifomu: Illumina NovaSeq 6000

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