Tseba ka bokhoni ba ho ithuta mefuta e sa tšoaneng ea lipatlisiso, e bonts'itsoeng ke taba ea bohlokoa ho tsoa ho bareki ba BMKGENE.Haufinyane tjena e hatisitsoeng khatisong ea selemo sena ea Plant Biotechnology Journal, sehlooho se reng "Chromosome-level Wild Hevea brasiliensis Genome: Empowering Genomic-Assisted Breeding and Unearthing Vital Loci bakeng sa Kotulo e Phahameng ea Rabara" e eme e le boitsebiso ba bohlokoa.
Sefate sa rabara sa Brazil (Hevea brasiliensis) se telele joalo ka mohloli oa bohlokoa oa tlhaho oa rabara.Ho pholletsa le lekholo la lilemo le fetileng, mekhoa e tloaelehileng ea ho ikatisa e ile ea etsa hore tlhahiso ea rabara e eketsehe ka makhetlo a tšeletseng.Leha ho le joalo, motheo oa liphatsa tsa lefutso o ikarabellang bakeng sa ntlafatso e ka bang teng ea lihlahisoa tsa rabara e ntse e le moferefere.
Boithuto bona bo qalile ka ho aha mofuta oa chromosome bakeng sa sefate sa rabara se hlaha, ho sebelisa tatellano ea tatellano ea nako e telele ea Nanopore, tatellano ea Illumina NGS, le theknoloji ea Hi-C.
Ka mokhoa o ts'oanang, pokello ea lisebelisoa tse 147 tsa likokoana-hloko, tse kenyelletsang mefuta e mengata ea lihlahisoa, mefuta e hlaha, le mefuta e amanang le eona, e ile ea bokelloa bakeng sa tlhahlobo e feletseng ea genome resequencing (WGS) ho sebelisoa sethala sa Illumina.Lintlha tse latelang tsa tatellano li ile tsa etsoa tlhahlobo e matla ea liphatsa tsa lefutso tsa baahi, e leng se ileng sa etsa hore ho be le karohano ea sebopeho sa baahi, mefuta-futa, le ho se leka-lekane ha likamano.Tlhahlobo ena e fana ka leseli mabapi le phapang ea malapa ho pholletsa le baahi.
Liphuputso tse ling li ne li kenyelletsa tlhahlobo e kenyelletsang Fst, π, Tajima's D, le LD, ka sepheo sa ho utolla mats'oao a khetho a hlahang poelong e ntlafalitsoeng ea latex, haholo-holo e shebaneng le li-clones tsa Wickham.
E fihlile sehlohlolong sa Genome-wide Association Analysis (GWAS), matšoao a amanang le litšobotsi tsa lihlahisoa le liphatsa tsa lefutso tse amanang le lihlahisoa li ile tsa totobatsoa.Netefatso ea pele e senotse liketsahalo tse ikhethang tsa mongolo ho mefuta e 'meli e fapaneng ea liphatsa tsa lefutso tsa ERF, e fana ka tlhase ea pele ea bohlokoa ba ts'ebetso ea tsona.Netefatso e eketsehileng e reretsoe lipatlisisong tse tlang.
BMKGENE e motlotlo ka ho kenya letsoho phetisong e pharalletseng ea liphatsa tsa lefutso tsa sechaba sena se seholo, hammoho le karolo e kholo tlhahlobisong ea bioinformatics.Boiphihlelo ba rona bo boholo bo akaretsa tatelano ea NGS/TGS le tlhahlobo ea bioinformatics ka mefuta e mengata ea 1000+.Re lebelletse tebello ea ho sebelisana le uena mererong ea hau e tlang.
Tobetsa mona hoithute haholoanyane ka thuto ena
Nako ea poso: Aug-29-2023