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Sequencing ea Chromatin Immunoprecipitation (ChIP-seq)

Chromatin Immunoprecipitation Sequencing (ChIP-seq) Setšoantšo se Featured

ChIP-Seq e fana ka profil ea genome-wide ea lipheo tsa DNA bakeng sa phetoho ea histone, lintlha tsa transcription, le liprotheine tse ling tse amanang le DNA.E kopanya khetho ea chromatin immuno-precipitation (ChIP) ho khutlisa protheine-DNA complexes, ka matla a tatellano ea moloko o latelang (NGS) bakeng sa tatellano e phahameng ea DNA e fumanoeng.Ho feta moo, kaha liprotheine-DNA complexes li fumanoa liseleng tse phelang, libaka tse tlamang li ka bapisoa ka mefuta e fapaneng ea lisele le lisele, kapa tlas'a maemo a sa tšoaneng.Likopo li fapana ho tloha taolong ea mongolo ho ea ho litsela tsa nts'etsopele ho isa mekhoeng ea mafu le ho feta.

Sethala: Illumina NovaSeq Platform

Iteanye le rona

Lintlha tsa Tšebeletso

Bioinformatics

Demo Results

Melemo ea Tšebeletso

● Sethala: Illumina NovaSeq Platform, PE150

● Mofuta oa laebrari: 100-500bp kenya laeborari ea DNA (ho itšetlehile ka kabo ea tlhōrō)

● Leano la ho latellana: Paired-End 150 bp

● Khothaletsa tlhahiso ea data: 6 Gb data/sample e tala.

Litlhoko tsa Mohlala

Palo ea DNA: ≥ 70 ng, tlhoro e kholo ea 100 ho isa ho 500 bp

Boholo ba mohlala: ≥ 10 μl

OD260/280 = 1.8 ho 2.0 ntle le ho senyeha kapa ho silafala ha RNA

Ts'ebetso ea liteko

Phallo ea Mosebetsi oa Ts'ebeletso

tlhahiso ea mohlala

Mohlala oa thomello

Tokiso ea Laebrari

Kaho ea laebrari

Tatelano

Tatelano

Tlhahlobo ea lintlha

Tlhahlobo ea lintlha

Ka mor'a ho rekisa Litšebeletso

Litšebeletso tsa ka mor'a thekiso

E fetileng: Metagenomic Sequencing -NGS

E 'ngoe: Tatelano e felletseng ea transcriptome - Illumina

1. Kabo ea ntlafatso ea tlhoro

2. Morero oa MA oa tlhōrō ea phapang

3.Tlhaloso ea KEGG

4. GO classification

fumana qotso

Ngola molaetsa wa hao mona mme o re romele wona

Sehlahisoalihlopha

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