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PacBio-Full-length 16S/18S/ITS Amplicon Sequencing
Sethala sa Amplicon (16S/18S/ITS) se entsoe ka boiphihlelo ba lilemo tsa tlhahlobo ea projeke ea mefuta-futa ea likokoana-hloko, e nang le tlhahlobo e tloaelehileng ea mantlha le tlhahlobo ea motho ka mong: tlhahlobo ea mantlha e akaretsa litaba tsa tlhahlobo e akaretsang ea lipatlisiso tsa hajoale tsa likokoana-hloko, litaba tsa tlhahlobo li ruile ebile li na le botlalo, le liphetho tsa tlhahlobo li hlahisoa ka mokhoa oa litlaleho tsa morero;Litaba tsa tlhahlobo ea motho ka mong li fapane.Mehlala e ka khethoa 'me liparamente li ka hlophisoa ka mokhoa o bonolo ho latela tlaleho ea tlhahlobo ea mantlha le sepheo sa lipatlisiso, ho fihlela litlhoko tsa motho ka mong.Sistimi ea ts'ebetso ea Windows, e bonolo ebile e potlakile.
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PacBio-Full-length Transcriptome (Non-Reference)
Ho nka data ea tatellano ea Isoform ea Pacific Bioscience (PacBio) joalo ka ho kenya, Sesebelisoa sena se khona ho tsebahatsa tatelano ea sengoloa sa bolelele bo felletseng (ntle le ho kopanya).Ka ho etsa 'mapa oa tatellano ea bolelele bo felletseng khahlano le genome ea litšupiso, lingoliloeng li ka ntlafatsoa ka liphatsa tsa lefutso tse tsebahalang, lingoliloeng, libaka tsa likhoutu, joalo-joalo. Tabeng ena, ho ka finyelloa boitsebiso bo nepahetseng haholoanyane ba meaho ea mRNA, joalo ka mefuta e meng ea ho kopanya, joalo-joalo.Tlhahlobo e kopaneng le data ea tatellano ea li-transcriptome ea NGS e etsa hore ho be le tlhaloso e felletseng le palo e nepahetseng haholoanyane ea polelo maemong a mongolo, e leng molemo haholo oa polelo e fapaneng le tlhahlobo ea ts'ebetso.
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Lisebelisoa tsa lisebelisoa
BMKCloud ke sethala se etelletseng pele sa bioinformatic se fanang ka tharollo ea sebaka se le seng bakeng sa mananeo a genomic, a tšeptjoang haholo ke bafuputsi mabaleng a fapaneng a kenyeletsang bongaka, temo, tikoloho, joalo-joalo. BMKCloud e na le lisebelisoa tse fapaneng tse sebelisoang khafetsa tsa bioinformatic tse kenyelletsang tlhaloso ea liphatsa tsa lefutso, lisebelisoa tsa liphatsa tsa lefutso, ncRNA, taolo ea boleng ba data, kopano, ho tsamaisana, ho ntšoa ha data, liphetoho, lipalo, jenereithara ea litšoantšo, tatellano. tlhahlobo, joalo-joalo.
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RNA e nyane
Li-RNA tse nyane ke mofuta oa RNA e khuts'oane e sa keneng likhoutu ka bolelele ba 18-30 nt, ho kenyeletsoa miRNA, siRNA le piRNA.Li-RNA tsena tse nyenyane li 'nile tsa tlalehoa ka bongata hore li ameha mekhoeng e fapaneng ea likokoana-hloko tse kang ho senyeha ha mRNA, ho thibela phetolelo, ho thehoa ha heterochromatin, joalo-joalo. Tlhahlobo ea tatellano ea SmallRNA e sebelisitsoe haholo lithutong tsa nts'etsopele ea liphoofolo / limela, maloetse, kokoana-hloko, joalo-joalo. Sequencing analysis platform e na le tlhahlobo e tloaelehileng le merafo e tsoetseng pele ea data.Motheong oa data ea RNA-seq, tlhahlobo e tloaelehileng e ka fihlela boitsebahatso le bonohe ba miRNA, ponelopele ea liphatsa tsa lefutso tsa miRNA, tlhaloso le tlhahlobo ea polelo.Tlhahlobo e tsoetseng pele e nolofalletsa ho batla le ho ntšoa ha miRNA, tlhahiso ea setšoantšo sa Venn, miRNA le mohaho oa marang-rang oa liphatsa tsa lefutso.
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NGS-WGS (Illumina/BGI)
NGS-WGS ke sethala se felletseng sa tlhahlobo ea tatellano ea liphatsa tsa lefutso, se ntlafalitsoeng motheong oa boiphihlelo bo bongata ho Biomarker Technologies.Sethala sena se bonolo sa ho se sebelisa se lumella ho fana ka potlako ha ts'ebetso e kopaneng ea tlhahlobo ka ho beha lintlha tse 'maloa tsa motheo, tse lumellanang le data ea tatellano ea DNA e hlahisitsoeng sethaleng sa Illumina le sethala sa tatellano sa BGI.Sethala sena se sebelisoa ho seva sa komporo ea ts'ebetso e phahameng, e matlafatsang tlhahlobo ea data e sebetsang hantle haholo ka nako e lekanyelitsoeng haholo.Merafo ea data ea motho ka mong e fumaneha ho latela tlhahlobo e tloaelehileng, ho kenyeletsoa potso ea mofuta o fetotsoeng, moralo oa mantlha oa PCR, jj.
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mRNA(Reference)
Transcriptome ke sehokelo lipakeng tsa tlhaiso-leseling ea liphatsa tsa lefutso le proteome ea ts'ebetso ea baeloji.Taolo ea boemo ba ho ngoloa ke mokhoa oa bohlokoa ka ho fetisisa le o ithutoang ka ho fetisisa oa taolo ea lintho tse phelang.Transcriptome sequencing e ka tatellano ea transcriptome ka nako leha e le efe ka nako kapa tlas'a boemo leha e le bofe, ka qeto e nepahetseng ho nucleotide e le 'ngoe. E ka bonahatsa ka mokhoa o ts'oanelang boemo ba liphatsa tsa lefutso, ka nako e le 'ngoe e khetholla le ho lekanya litlaleho tse sa tloaelehang le tse tloaelehileng,' me e fane ka boitsebiso ba sebopeho sa mohlala oa litšoantšo tse khethehileng.
Hajoale, thekenoloji ea tatellano ea li-transcriptome e sebelisitsoe haholo ho agronomy, bongaka le mafapheng a mang a lipatlisiso, ho kenyeletsoa taolo ea nts'etsopele ea liphoofolo le limela, ho ikamahanya le maemo a tikoloho, tšebelisano ea 'mele ea ho itšireletsa mafung, tikoloho ea liphatsa tsa lefutso, ho iphetola ha liphatsa tsa lefutso le hlahala le ho lemoha lefu la lefutso.
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Metagenomics (NGS)
Sethala sena sa tlhahlobo se etselitsoe tlhahlobo ea data ea shotgun metagenomic motheong oa boiphihlelo ba lilemo.E na le ts'ebetso e kopantsoeng ea ts'ebetso e nang le litlhahlobo tse fapaneng tsa metagenomics tse hlokahalang hangata ho kenyelletsa ts'ebetso ea data, lithuto tsa boemo ba mefuta, lithuto tsa boemo ba liphatsa tsa lefutso, binning ea metagenome, joalo-joalo. Ho feta moo, lisebelisoa tsa morafo tsa data tse hlophisitsoeng li fumaneha holim'a tlhahlobo e tloaelehileng ea mosebetsi, ho kenyeletsoa potso ea mofuta le mefuta. , tlhophiso ea paramethara, tlhahiso ea motho ka mong, joalo-joalo.
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LncRNA
Li-RNA tse telele tse sa khouteng (lncRNA) ke mofuta oa lingoliloeng tse nang le bolelele bo fetang 200 nt, tse sa khoneng ho khouta liprotheine.Bopaki bo ipokeletseng bo fana ka maikutlo a hore boholo ba li-lncRNA li na le monyetla oa ho sebetsa.Litheknoloji tsa tatellano ea maemo a holimo le lisebelisoa tsa tlhahlobo ea bioinformatic li re fa matla a ho senola tatelano ea lncRNA le ho beha tlhahisoleseling ka katleho le ho re lebisa ho sibolleng li-lncRNA tse nang le mesebetsi ea bohlokoa ea taolo.BMKCloud e motlotlo ho fa bareki ba rona sethala sa tlhahlobo ea lncRNA ho fihlela tlhahlobo e potlakileng, e tšepahalang le e bonolo ea lncRNA.
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GWASE
Boithuto ba Genome-wide Association (GWAS) bo ikemiselitse ho khetholla mefuta e fapaneng ea liphatsa tsa lefutso (genotype) e amanang le litšobotsi tse itseng (phenotype).Lithuto tsa GWA li etsa lipatlisiso tsa matšoao a liphatsa tsa lefutso tse tšelang palo e kholo ea batho ka bomong le ho bolela esale pele mekhatlo ea genotype-phenotype ka tlhahlobo ea lipalo-palo boemong ba baahi.Whole-genome resequencing e ka khona ho sibolla mefuta eohle ea liphatsa tsa lefutso.Ho kopantsoe le lintlha tsa phenotypic, GWAS e ka sebetsoa ho khetholla li-SNP tse amanang le phenotype, li-QTL le liphatsa tsa lefutso tsa likhetho, tse tšehetsang ka matla ho ikatisa ha liphoofolo / limela tsa sejoale-joale.SLAF ke leano le nolofalitsoeng la tatellano ea liphatsa tsa lefutso, le sibollang matšoao a pharalletseng a pharalletseng a genome, SNP.Li-SNP tsena, e le matšoao a liphatsa tsa lefutso tsa molek'hule, li ka sebetsoa bakeng sa lithuto tsa kopanelo tse nang le litšobotsi tse lebisitsoeng.Ke leano le bolokang chelete e ngata ho tsebahatsa litšobotsi tse rarahaneng tse amanang le liphatsa tsa lefutso.
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Nanopore Tlhōlo e feletseng ea transcriptomics
Li-isoform tse rarahaneng le tse feto-fetohang linthong tse phelang ke mekhoa ea bohlokoa ea liphatsa tsa lefutso bakeng sa ho laola liphatsa tsa lefutso le ho fapana ha liprotheine.Khethollo e nepahetseng ea meaho ea mongolo ke motheo oa boithuto bo tebileng ba mekhoa ea taolo ea polelo ea lefutso.Sethala sa tatellano sa Nanopore se atlehile ho tlisa boithuto ba transcriptomic boemong ba isoform.Sethala sena sa tlhahlobo se etselitsoe ho sekaseka data ea RNA-Seq e hlahisitsoeng sethaleng sa Nanopore motheong oa genome ea referense, e fihlelang tlhahlobo ea boleng le palo maemong a liphatsa tsa lefutso le boemo ba lingoliloeng.
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selikalikoe-RNA
CircRNA (circRNA) ke mofuta oa RNA e sa ngolisoeng, e sa tsoa fumanoa e phetha karolo ea bohlokoa ho marang-rang a taolo a amehang ho nts'etsopele, ho hanyetsa tikoloho, joalo-joalo E fapane le limolek'hule tsa RNA tse nang le mela, mohlala, mRNA, lncRNA, 3′ le 5′. lipheletso tsa circRNA li kopantsoe hammoho ho etsa sebopeho se chitja, se li pholosang ho tsoa tšilong ea exonuclease mme li tsitsitse ho feta boholo ba RNA e melang.CircRNA e fumanoe e na le mesebetsi e fapaneng ea ho laola polelo ea lefutso.CircRNA e ka sebetsa joalo ka ceRNA, e tlamang miRNA ka tlholisano, e tsejoang ka seponche sa miRNA.Sethala sa tlhahlobo ea tatellano ea CircRNA se matlafatsa sebopeho sa circRNA le tlhahlobo ea polelo, ponelopele ea sepheo le tlhahlobo e kopaneng le mefuta e meng ea limolek'hule tsa RNA.
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BSA
Sethala sa Bulked Segregant Analysis se na le tlhahlobo e tloaelehileng ea mohato o le mong le tlhahlobo e tsoetseng pele e nang le maemo a ikhethileng a paramente.BSA ke mokhoa o sebelisoang ho tsebahatsa matšoao a lefutso a amanang le phenotype.Ts'ebetso e kholo ea BSA e na le: 1. ho khetha lihlopha tse peli tsa batho ba nang le phenotypes tse hanyetsanang haholo;2. ho kopanya DNA, RNA kapa SLAF-seq(E ntlafalitsoe ke Biomarker) ea batho bohle ho etsa bongata bo boholo ba DNA;3. ho khetholla tatellano e fapaneng khahlanong le genome ea litšupiso kapa lipakeng, 4. ho bolela esale pele libaka tse amanang le mokhethoa ka ED le SNP-index algorithm;5. Tlhahlobo ea ts'ebetso le ho ntlafatsa liphatsa tsa lefutso libakeng tsa bo-nkgetheng, joalo-joalo. Meepo e tsoetseng pele haholoanyane litabeng tse kenyeletsang tlhahlobo ea liphatsa tsa lefutso le moralo oa pele le tsona lia fumaneha.
