Nako e telele e sa ngodiseng Sequencing-Illumina
Melemo ea Tšebeletso
●Tlhahlobo e kopaneng ea mRNA le lncRNA: ka ho kopanya quantification ea mRNA transcripts le thuto ea lncRNA le lipakane tsa bona, hoa khoneha ho fumana tlhaloso e tebileng ea mokhoa oa ho laola o itšetlehileng ka karabo ea cellular.
●Tsebo e Pharalletseng: Sehlopha sa rona se tlisa boiphihlelo bo bongata morerong o mong le o mong, ka rekoto ea ho sebetsa lisampole tse fetang 23,000 ho BMK e nkang mefuta e fapaneng ea sampole le merero ea lncRNA.
●Taolo ea Boleng e Tiileng: Re kenya tšebetsong lintlha tsa mantlha tsa taolo methating eohle, ho tloha ho lisampole le boitokiso ba laeborari ho isa ho tatellano le bioinformatics. Tlhokomelo ena e hlokolosi e tiisa hore ho fanoa ka liphetho tsa boleng bo holimo kamehla.
●Tšehetso ea Post-Sales: Boitlamo ba rona bo fetela ka nģ'ane ho ho phethoa ha morero ka nako ea tšebeletso ea likhoeli tse 3 ka mor'a thekiso. Nakong ena, re fana ka tatellano ea projeke, thuso ea ho rarolla mathata, le linako tsa Q&A ho araba lipotso life kapa life tse amanang le sephetho.
Litlhoko tsa Mohlala le Phano
| Laebrari | Sethala | Lintlha tse khothalelitsoeng | Lintlha tsa QC |
| rRNA e feletsoe ke laebrari ea tataiso | Illumina PE150 | 10-16 Gb | Q30≥85% |
Li-nucleotides:
| Conc.(ng/μl) | Chelete (μg) | Bohloeki | Botšepehi |
| ≥80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Tšoaetso e fokolang kapa ha e na protheine kapa DNA e bontšitsoeng ho gel. | RIN≥6.0; 5.0≥28S/18S≥1.0; bophahamo bo fokolang kapa bo se nang motheo |
● Limela:
Motso, Stem kapa Petal: 450 mg
Lekhasi kapa Peō: 300 mg
Litholoana: 1.2 g
● Phoofolo:
Pelo kapa mala: 450 mg
Viscera kapa Boko: 240 mg
mesifa: 600 mg
Masapo, Moriri kapa Letlalo: 1.5g
● Arthropods:
Likokoanyana: 9g
Crustacea: 450 mg
● Mali a feletseng:2 li-tubes
● Lisele: 106 lisele
● Serum le Plasma6ml ka
Tlhahiso ea Mohlala e khothalelitsoeng
Setshelo: 2 ml centrifuge tube (Tin foil ha e khothalletsoe)
Mohlala oa ho ngola: Sehlopha+katiso mohlala A1, A2, A3; B1, B2, B3.
Thomello:
1. Leqhoa le omileng: lisampole li lokela ho kenngoa ka mekotleng ebe li patoa ka leqhoa le omileng.
2. Li-tubes tsa RNAstable: lisampole tsa RNA li ka omisoa ka har'a tube ea ho tsitsisa ea RNA (mohlala, RNAstable®) le ho romelloa ka mocheso oa kamore.
Phallo ea Mosebetsi oa Ts'ebeletso
Moqapi oa liteko
Mohlala oa thomello
Tlhahiso ea RNA
Kaho ea laebrari
Tatelano
Tlhahlobo ea lintlha
Litšebeletso tsa ka mor'a thekiso
Bioinformatics
Tlhahlobo e fapaneng ea Gene Expression (DEGs).
Quantification ea polelo ea lncRNA - ho kopanya
Ntlafatso ea liphatsa tsa lefutso tsa lncRNA
Tlhatlhobo e kopaneng ea mRNA le lncRNA - Morero oa Circos (sedikadikwe se bohareng ke mRNA mme cicrlce e ka hare ke lncRNA)
Lekola tsoelopele e tsamaisoang ke litšebeletso tsa BMKGene's lncRNA ka pokello e hlophisitsoeng ea lingoliloeng.
Ji, H. et al. (2020) 'Boitsebiso, ho bolela esale pele ka ts'ebetso, le netefatso ea bohlokoa ea lncRNA ea li-lncRNA tse amanang le khatello ea maikutlo ka har'a sebete sa likhoto', Litlaleho tsa Saense 2020 10: 1, 10 (1), maq. 1-14. doi: 10.1038/s41598-020-57451-7.
Jia, Z. et al. (2021) 'Tlhahlobo e Kopanetsoeng ea Transcriptomic e Senola Mokhoa oa ho itšireletsa mafung bakeng sa CyHV-3-Resistant Common Carp Strain', Frontiers in Immunology, 12, leq. 687151. doi: 10.3389/FIMMU.2021.687151/BIBTEX.
Wang, XJ le ba bang. (2022) 'Multi-Omics Integration-Based Prioritization of Competing Endogenous RNA Regulation Networks in Small Cell Lung Cancer: Litšobotsi tsa Limolek'hule le Bakhethoa ba Lithethefatsi', Frontiers in Oncology, 12, leq. 904865. doi: 10.3389/FONC.2022.904865/BIBTEX.
Xiao, L. et al. (2020) 'Genetic dissection of the gene coexpression network underlying photosynthesis in Populus', Plant Biotechnology Journal, 18 (4), pp. 1015-1026. doi: 10.1111/PBI.13270.
Zheng, H. et al. (2022) 'Global Regulatory Network for Dysregulated Gene Expression and Abnormal Metabolic Signaling in Immune Cells in the Microenvironment of Graves' Disease le Hashimoto's Thyroiditis ', Frontiers in Immunology, 13, leq. 879824. doi: 10.3389/FIMMU.2022.879824/BIBTEX.
