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Genome-wide Association Analysis
Boithuto ba Genome-wide Association (GWAS) bo ikemiselitse ho khetholla mefuta e fapaneng ea liphatsa tsa lefutso (genotype) e amanang le litšobotsi tse itseng (phenotype).Boithuto ba GWAS bo batlisisa matšoao a liphatsa tsa lefutso tse tšelang palo e kholo ea batho ka bomong le ho bolela esale pele mekhatlo ea genotype-phenotype ka tlhahlobo ea lipalo maemong a baahi.E sebelisitsoe haholo lipatlisisong tsa mafu a batho le merafo e sebetsang ea liphatsa tsa lefutso ka litšobotsi tse rarahaneng tsa liphoofolo kapa limela.
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Sequloana se le seng sa RNA Sequencing
Tsoelo-pele ea ho nka sele e le 'ngoe le mokhoa oa ho haha laebrari ka bomong ho kopanngoa le tatellano e phahameng e lumellang lithuto tsa liphatsa tsa lefutso motheong oa sele-by-cell.E etsa hore ho be le tlhahlobo e tebileng le e felletseng ea litsamaiso tsa lisele tse rarahaneng, moo haholo-holo e qobang ho pata liphapang tsa bona ka ho nka karolelano ea lisele tsohle.
Leha ho le joalo, lisele tse ling ha li tšoanelehe ho etsoa ho emisoa ha sele e le 'ngoe, kahoo mekhoa e meng ea ho lokisa sampole - ho ntšoa ha nucleus ho tsoa liseleng hoa hlokahala, ke hore, nucleus e ntšoa ka kotloloho ho lisele kapa lisele ebe e lokisetsoa ho emisoa ha nucleus e le 'ngoe bakeng sa motho a le mong. tatellano ea lisele.
BMK e fana ka ts'ebeletso ea tatellano ea 10× Genomics ChromiumTM e thehiloeng ho sele e le 'ngoe ea RNA.Ts'ebeletso ena e sebelisitsoe haholo lithutong tse amanang le mafu, joalo ka phapang ea lisele tsa 'mele oa ho itšireletsa mafung, tumor heterogeneity, nts'etsopele ea lisele, joalo-joalo.
Spatial transcriptome chip: 10 × Genomics
Sethala: Illumina NovaSeq 6000
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Tatelano ya Genome ya Semela/Aphoofolo
Whole genome re-sequencing, eo hape e tsejoang e le WGS, e thusa ho senola liphetoho tse tloaelehileng le tse sa tloaelehang ho genome eohle ho kenyelletsa Single Nucleotide Polymorphism (SNP), Insertion Deletion (InDel), Structure variation (SV), le Copy Number Variation (CNV). ).Li-SV li etsa karolo e kholoanyane ea motheo oa ho fapana ho feta li-SNP 'me li na le tšusumetso e kholo ho genome, e nang le phello e kholo ho lintho tse phelang.Requencing ea nako e telele e lumella ho tsebahatsa ka nepo likhechana tse kholo le liphapang tse rarahaneng hobane ho bala nako e telele ho etsa hore ho be bonolo haholo ho tšela chromosomal libakeng tse rarahaneng joalo ka ho pheta-pheta tandem, libaka tse ruileng tsa GC / AT, le libaka tse feto-fetohang.
Sethala: Illumina, PacBio, Nanopore
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BMKMANU S1000 Spatial Transcriptome
Ho hlophisoa ha sebaka sa lisele ho phetha karolo ea bohlokoa lits'ebetsong tse fapaneng tsa baeloji, joalo ka ho kenella ha 'mele oa ho itšireletsa mafung, nts'etsopele ea embryo, joalo-joalo. Sequencing ea Spatial transcriptome, e bonts'ang profiling ea liphatsa tsa lefutso ha ho ntse ho bolokoa tlhahisoleseling ea boemo ba sebaka, e fana ka leseli le leholo mabapi le meaho ea lithane tsa transcriptome.Ka nts'etsopele ea thekenoloji, morphology ea lithane tse hlakileng haholo le phapang ea 'nete ea sebopeho sa sebaka sa polelo ea limolek'hule li hloka ho ithutoa ka qeto e phahameng.BMKGENE e fana ka ts'ebeletso e felletseng, e emisang e le 'ngoe ea tatellano ea litaba tsa sebaka ho tloha lisampoleng ho ea ho leseli la baeloji.
Mahlale a transcriptomics a Spatial a matlafalitse litebello tse ncha mabaleng a lipatlisiso a fapaneng ka ho rarolla boemo ba polelo ea mofuta o nang le litaba tsa sebaka ka lisampole tse fapaneng.
Chip ea transcriptome ea sebaka: BMKMANU S1000
Sethala: Illumina NovaSeq 6000
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10x Genomics Visium Spatial Transcriptome
Visium Spatial Gene Expression ke tekhenoloji e tloaelehileng ea tatellano ea sebaka sa transcriptome bakeng sa ho arola lisele tse thehiloeng ho mRNA kaofela.'Mapa oa mongolo o felletseng o nang le moelelo oa morphological ho fumana lintlha tse ncha mabapi le nts'etsopele e tloaelehileng, lefu la mafu, le lipatlisiso tsa phetolelo ea bongaka.BMKGENE e fana ka ts'ebeletso e felletseng, e emisang e le 'ngoe ea tatellano ea litaba tsa sebaka ho tloha lisampoleng ho ea ho leseli la baeloji.
Theknoloji ea Spatial transcriptomics e matlafalitse litebello tse ncha mabaleng a lipatlisiso a fapaneng ka ho rarolla boemo ba polelo ea mofuta o nang le litaba tsa sebaka ka lisampole tse fapaneng..
Chip ea transcriptome ea sebaka: 10x Genomics Visium
Sethala:Illumina NovaSeq 6000
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Bolelele bo Feletseng ba mRNA Sequencing-Nanopore
Tatelano ea RNA e bile sesebelisoa sa bohlokoahali bakeng sa tlhahlobo e felletseng ea transcriptome.Ntle le pelaelo, tatelano ea khale ea ho bala nako e khuts'oane e fihletse ntlafatso e mengata ea bohlokoa mona.Leha ho le joalo, hangata e kopana le mefokolo ho boitsebahatso ba isoform ea bolelele bo felletseng, quantification, leeme la PCR.
Nanopore sequencing e ikhetholla ho li-platform tse ling tse latellanang, ka hore li-nucleotide li baloa ka ho toba ntle le motsoako oa DNA 'me li hlahisa nako e telele ho bala li-kilobase tse mashome.Sena se matlafatsa sengoloa sa bolelele bo felletseng ba ho bala ka kotloloho le ho sebetsana le liphephetso lithutong tsa boemo ba isoform.
Sethala:Nanopore PromethION
Laeborari:cDNA-PCR
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Tatelano ya bolelele bo felletseng ba mRNA -PacBio
Ho fihlela joalefull-length transcriptome sequencing, e tsejoang hape e leHo fihlela joaleIso-Seq e nka melemo ea sequencer ea PacBio ka bolelele ba ho bala, e nolofalletsang tatellano ea limolek'hule tsa cDNA tsa bolelele bo felletseng ntle le khefu.Sena se qoba ka ho feletseng liphoso leha e le life tse hlahisoang mehatong ea kopano e ngotsoeng 'me se etsa li-unigene tse nang le qeto ea boemo ba isoform.Sehlopha sena sa unigene se fana ka tlhahisoleseding e matla ea liphatsa tsa lefutso e le "reference genome" ho transcriptome-level.Ntle le moo, ho kopantsoe le data ea tatellano ea moloko o latelang, ts'ebeletso ena e matlafatsa quantification e nepahetseng ea polelo ea boemo ba isoform.
Sethala: PacBio Sequel IILaebrari: Laeborari ea tšepe ea SMRT -
Eukaryotic mRNA Sequencing-Illumina
tatellano ea mRNA e nolofalletsa profil ea li-mRNA tsohle tse ngotsoeng ho tsoa liseleng tlas'a maemo a itseng.Ke thekenoloji e matla ea ho senola boemo ba liphatsa tsa lefutso, mekhoa ea liphatsa tsa lefutso le mekhoa ea limolek'hule ea mekhoa e itseng ea likokoana-hloko.Ho fihla joale, tatellano ea mRNA e sebelisitsoe haholo lipatlisisong tsa mantlha, tlhahlobo ea bongaka, nts'etsopele ea lithethefatsi, jj.
Sethala: Illumina NovaSeq 6000
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Non-Reference based mRNA Sequencing-Illumina
tatellano ea mRNA e sebelisa mokhoa oa tatellano oa moloko o latelang (NGS) ho hapa lenģosa la RNA(mRNA) sebopeho sa Eukaryote ka nako e itseng eo mesebetsi e meng e khethehileng e ntseng e sebetsa.Sengoliloeng se selelele ka ho fetesisa se ne se bitsoa 'Unigene' 'me se sebelisitsoe e le tatelano ea litšupiso bakeng sa tlhahlobo e latelang, e leng mokhoa o sebetsang oa ho ithuta mokhoa oa limolek'hule le marang-rang a taolo ea mefuta ntle le ho buuoa.
Ka mor'a kopano ea data ea transcriptome le tlhaloso e sebetsang ea unigene
(1) Tlhahlobo ea SSR, ho bolela esale pele ka CDS le sebopeho sa liphatsa tsa lefutso li tla etsoa esale pele.
(2) Quantification ea unigene expression ho sampole ka 'ngoe e tla etsoa.
(3) Li-unigenes tse hlahisitsoeng ka mokhoa o fapaneng lipakeng tsa lisampole (kapa lihlopha) li tla fumanoa ho ipapisitsoe le polelo e fapaneng.
(4) Ho tla etsoa tlhahlobo ea lihlopha, tlhaloso e sebetsang le tlhahlobo ea ntlafatso ea li-unigenes tse hlalositsoeng ka tsela e fapaneng.
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Tatelano ya nako e telele e sa khoutong-Illumina
Li-RNA tse telele tse sa khouteng (lncRNAs) ke mofuta oa limolek'hule tsa RNA tse bolelele bo fetang 200 nt, tse nang le bokhoni bo tlase haholo ba ho khouta.LncRNA, joalo ka setho se ka sehloohong ho li-RNA tse sa khouteng, e fumanoa haholo ka har'a nucleus le plasma.Nts'etsopele ea tatellano ea thekenoloji le bioinformatics e thusa ho tsebahatsa li-lncRNA tse ngata le ho amahanya tse nang le mesebetsi ea tlhaho.Bopaki bo bokelletsoeng bo fana ka maikutlo a hore lncRNA e ameha haholo ho epigenetic regulation, transcription regulation le post-transcription regulation.
