Tlhahlobo ea Mokhatlo oa Genome ka Bophara
Mokgwa wa mosebetsi
Melemo ea Tšebeletso
●Bokgoni bo pharaletseng le direkoto tsa phatlalatso: ka boiphihlelo bo bokelletsoeng ho GWAS, BMKGene e phethetse merero e makholo ea mefuta ea liphoofolo lipatlisisong tsa baahi ba GWAS, e thusitse bafuputsi ho phatlalatsa lingoloa tse fetang 100, 'me tšusumetso e kopaneng e fihlile ho 500.
● Tlhahlobo e felletseng ea bioinformatics: mokhoa oa tšebetso o kenyelletsa tlhahlobo ea kamano ea litšobotsi tsa SNP, e fanang ka sete ea liphatsa tsa lefutso tse ka khethoang le tlhaloso ea tsona ea ts'ebetso e tsamaellanang.
●Sehlopha se nang le boiphihlelo bo phahameng ba bioinformatics le potoloho e khutšoanyane ea tlhahlobo: ka boiphihlelo bo boholo tlhahlobong e tsoetseng pele ea genomics, sehlopha sa BMKGene se fana ka tlhahlobo e felletseng ka nako e potlakileng ea phetoho.
●Tšehetso ea Kamora Thekiso:Boitlamo ba rona bo fetela ka nģ'ane ho ho phethoa ha projeke ka nako ea tšebeletso ea likhoeli tse 3 kamora thekiso. Nakong ena, re fana ka tlhahlobo ea projeke, thuso ea ho rarolla mathata, le lipuisano tsa lipotso le likarabo ho araba lipotso life kapa life tse amanang le liphetho.
Litlhaloso le litlhoko tsa tšebeletso
| Mofuta oa tatellano | Sekala se kgothaletswang sa baahi | Leano la ho latellana ha tatellano | Litlhoko tsa Nucleotide |
| Tatellano e Felletseng ea Genome | Disampole tse 200 | 10x | Ho tsepamisa maikutlo: ≥ 1 ng/ µL Kakaretso ea chelete ≥ 30ng Ho senyeha kapa ho silafala ho lekanyelitsoe kapa ha ho na hona |
| Sekhechana se Entsoeng ka Sebaka se Ikhethang (SLAF) | Botebo ba tag: 10x Palo ea li-tag: < 400 Mb: Ho kgothaletswa hore WGS e sebetse < 1Gb: Li-tag tse 100K 1Gb > 2Gb: Li-tag tse 300K Li-tag tse phahameng ka ho fetisisa tsa 500k | Ho tsepamisa maikutlo ≥ 5 ng/µL Kakaretso ea chelete ≥ 80 ng Nanodrop OD260/280=1.6-2.5 Jeli ea Agarose: ha e na kapa ha e na ho senyeha kapa ho silafala ho lekanyelitsoeng
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Khetho ea Lintho
Mefuta e fapaneng, mefuta e sa tsoaneng, dibaka tsa naha/dibanka tsa lefutso/malapa a tswakilweng/mehlodi ya naha
Mefuta e fapaneng, mefuta e meng, libaka tsa tlhaho
Lelapa la bana ba babedi/lelapa la bana ba babedi/mehlodi ya naha
Phallo ea Mosebetsi oa Tšebeletso
Moralo oa liteko
Phano ea mohlala
Ho ntšoa ha RNA
Kaho ea laeborari
Tatellano
Tlhahlobo ea lintlha
Litšebeletso tsa kamora thekiso
E kenyelletsa tlhahlobo e latelang:
- Tlhahlobo ea kamano ea liphatsa tsa lefutso ka bophara: mohlala oa LM, LMM, EMMAX, FASTLMM
- Tlhaloso e sebetsang ea liphatsa tsa lefutso tse ka khethoang
Tlhahlobo ea kamano ea litšobotsi tsa SNP - morero oa Manhattan
Tlhahlobo ea kamano ea litšobotsi tsa SNP - morero oa QQ
Hlahloba tsoelo-pele e tsamaisoang ke lits'ebeletso tsa BMKGene's de GWAS ka pokello ea lingoliloeng tse hlophisitsoeng hantle:
Lv, L. et al. (2023) 'Temohisiso motheong oa liphatsa tsa lefutso oa mamello ea ammonia ho razor clam Sinonovacula constricta ka thuto ea kopanelo ea genome ka bophara',Temo ea Metsing, 569, leq. 739351. doi: 10.1016/J.AQUACULTURE.2023.739351.
Li, X. et al. (2022) 'Litlhahlobo tsa multi-omics tsa li-accession tse 398 tsa foxtail millet li senola libaka tsa genomic tse amanang le ho rua, litšobotsi tsa metabolite, le litlamorao tse khahlanong le ho ruruha',Semela sa limolek'hule, 15(8), leqepheng la 1367-1383. doi: 10.1016/j.molp.2022.07.003.
Li, J. et al. (2022) ''Mapa oa Mokhatlo oa Li-genome-Wide oa Li-phenotype tse se nang Li-Hullless Barely Tikolohong ea Komello',Meeli ea Saense ea Limela, 13, leq. 924892. doi: 10.3389/FPLS.2022.924892/BIBTEX.
Zhao, X. et al. (2021) 'GmST1, e kenyang sulfotransferase, e fana ka khanyetso ho mefuta ea kokoana-hloko ea mosaic ea soya G2 le G3',Semela, Sele le Tikoloho, 44(8), leqepheng la 2777-2792. doi: 10.1111/PCE.14066.
