Genomics e Bapisang
Melemo ea Tšebeletso
●Bokgoni bo pharaletseng le direkoto tsa phatlalatso: ka ho bokellana, BMKGene e phethetse diporojeke tse fetang 90 tsa genomics tse bapiswang, ka ntlha ya tshusumetso e kopaneng e fihlileng ho 900.
●Tlhahlobo e felletseng ea bioinformatics: sephutheloana sa tlhahlobo se na le litlhahlobo tse robeli tse hlokahalang haholo, tse fanang ka lipalo tse hlophisitsoeng hantle tse loketseng ho phatlalatsoa le ho lumella tlhaloso e bonolo ea liphetho
●Sehlopha se nang le boiphihlelo bo phahameng ba bioinformatics le potoloho e khutšoanyane ea tlhahlobo: ka boiphihlelo bo boholo tlhahlobong ea genomics ea papiso, sehlopha sa BMKGene se phethahatsa litlhoko tse fapaneng tsa tlhahlobo ea botho ka nako e khuts'oane ea phetoho.
●Tšehetso ea Kamora Thekiso:Boitlamo ba rona bo fetela ka nģ'ane ho ho phethoa ha projeke ka nako ea tšebeletso ea likhoeli tse 3 kamora thekiso. Nakong ena, re fana ka tlhahlobo ea projeke, thuso ea ho rarolla mathata, le lipuisano tsa lipotso le likarabo ho araba lipotso life kapa life tse amanang le liphetho.
Litlhaloso tsa Tšebeletso
| Nako e hakanyetsoang ea ho reteleha | Palo ea mefuta | Litlhahlobo |
| Matsatsi a 30 a mosebetsi | 6 - 12 | Ho bokellana ha malapa a liphatsa tsa lefutso Katoloso le ho fokotseha ha lelapa la liphatsa tsa lefutso Kaho ea sefate sa phylogenetic Khakanyo ea nako ea ho fapana (Ho hlokahala tlhahlobo ea mesaletsa ea lintho tsa khale) Nako ea ho kenya LTR (Bakeng sa limela) Ho pheta-phetoa ha liphatsa tsa lefutso kaofela (Bakeng sa limela) Khatello e khethileng Tlhahlobo ea synteny |
Litlhahlobo tsa Bioinformatics
● Lelapa la liphatsa tsa lefutso
● Thuto ea liphatsa tsa lefutso
● Nako ea ho fapana
● Khatello e khethileng
● Tlhahlobo ea synteny
Litlhoko tsa Sampole le Phano
Litlhoko tsa Sampole:
Dinama kapa DNA bakeng sa ho hlophisa le ho kopanya genome
Bakeng sa Dinama
| Mefuta | Letlalo | Phuputso | PacBio CCS |
| Phoofolo | Lisele tsa visceral | 0.5 ~ 1 g | ≥ 3.5 g |
| Lisele tsa mesifa | |||
| ≥ 5.0 g | |||
| ≥ 5.0 mL | |||
| Mali a liphoofolo tse anyesang | |||
| ≥ 0.5 mL | |||
| Khoho/Mali a tlhapi | |||
| Semela | Lekhasi le Lecha | 1 ~ 2 g | ≥ 5.0 g |
| Lehlaku/Thupa | 1 ~ 2 g | ≥ 10.0 g | |
| Motso/Peo | 1 ~ 2 g | ≥ 20.0 g | |
| Lisele | Sele e holisitsoeng | - | ≥ 1 x 108 |
Lintlha
Lifaele tsa tatellano ea liphatsa tsa lefutso (.fasta) le lifaele tsa tlhaloso (.gff3) tsa mefuta e amanang haufi-ufi
Phallo ea Mosebetsi oa Tšebeletso
Moralo oa liteko
Phano ea mohlala
Kaho ea laeborari
Tatellano
Tlhahlobo ea lintlha
Litšebeletso tsa kamora thekiso
*Liphetho tsa demo tse bontšitsoeng mona kaofela li tsoa ho li-genome tse phatlalalitsoeng ka Biomarker Technologies
1.Khakanyo ea nako ea ho kenya ea LTR: Setšoantšo se bontšitse kabo e ikhethang ea bimodal linakong tsa ho kenya tsa LTR-RT ho genome ea rye ea Weining, ha e bapisoa le mefuta e meng. Tlhōrō ea morao tjena e hlahile lilemong tse ka bang limilione tse 0.5 tse fetileng.
Li Guang le ba bang,Liphatsa tsa lefutso tsa tlhaho, 2021
2. Tlhahlobo ea lelapa la phylogeny le liphatsa tsa lefutso mabapi le chayote (Sechium edule): Ka ho sekaseka chayote le mefuta e meng e 13 e amanang le eona lelapeng la liphatsa tsa lefutso, Chayote e fumanoe e amana haufi-ufi le noha ea sekho (Trichosanthes anguina). Chayote e nkiloeng ho noha ea sekho ka Mya e ka bang 27-45 le phetoho eohle ea liphatsa tsa lefutso (WGD) e ile ea bonoa ho chayote ka Mya ea 25±4, e leng ketsahalo ea boraro ea WGD ho cucuibitaceae.
Fu A le ba bang,Patlisiso ea Temo ea Lijalo, 2021
3. Tlhahlobo ea synteny: Lijini tse ling tse amanang le lihormone tsa phyto nts'etsopele ea litholoana li fumanoe ho chayote, snake gourd le squash. Kamano pakeng tsa chayote le squash e phahame hanyane ho feta pakeng tsa chayote le snake gourd.
Fu A le ba bang,Patlisiso ea Temo ea Lijalo, 2021
4. Tlhahlobo ea lelapa la liphatsa tsa lefutso: Ho rua molemo ha KEGG ka katoloso le ho fokotseha ha lelapa la liphatsa tsa lefutso li-genome tsa G.thurberi le G.davidsonii ho bontšitse hore liphatsa tsa lefutso tse amanang le biosynthesis ea steroid le brassinosteroid li atolositsoe.
Yang Z le ba bang,Thuto ea Baeloji ea BMC, 2021
5. Tlhahlobo ea ho pheta-pheta ha liphatsa tsa lefutso kaofela: Tlhahlobo ea kabo ea 4DTV le Ks e bontšitse ketsahalo ea ho pheta-pheta ha liphatsa tsa lefutso kaofela. Litlhōrō tsa mefuta e sa tšoaneng li bontšitse liketsahalo tsa ho pheta-pheta. Litlhōrō tsa mefuta e sa tšoaneng li bontšitse liketsahalo tsa ho iketsetsa. Tlhahlobo e bontšitse hore ha e bapisoa le mefuta e meng e meraro e amanang haufi-ufi, O. europaea e fetile ho pheta-pheta ha liphatsa tsa lefutso ka tekanyo e kholo haufinyane tjena.
Rao G le ba bang,Patlisiso ea Temo ea Lijalo, 2021
Nyeoe ea BMK
Rosa e se nang prickle: temohisiso ea liphatsa tsa lefutso e hokahane le ho ikamahanya le mongobo
E phatlalalitsoe: Tlhahlobo ea Saense ea Naha, 2021
Leano la ho latellana ha lintho:
'Basye'sThornless' (R.Wichurainan) genome:
Hoo e ka bang 93 X PacBio + hoo e ka bang 90 X Nanopore + 267 X Illumina
Liphetho tsa bohlokoa
1. Genome ea boleng bo holimo ea R.wichuraiana e hahiloe ho sebelisoa mekhoa ea tatellano e baliloeng nako e telele, e hlahisang kopano ea 530.07 Mb (Boholo ba genome bo hakantsoeng e ne e le hoo e ka bang 525.9 Mb ka cytometry ea phallo le 525.5 ka lipatlisiso tsa genome； Heterozygosity e ne e le hoo e ka bang 1.03%). Sephetho se hakantsoeng sa BUSCO e ne e le 93.9%. Ha se bapisoa le "Old blush" (haploOB), boleng le botlalo ba genome ena bo tiisitsoe ka ho nepahala ha motheo o le mong le index ea kopano ea LTR (LAI=20.03). Genome ea R.wichuraiana e na le liphatsa tsa lefutso tse 32,674 tse ngolang liprotheine.
2. Tlhahlobo ea manonyeletso a mangata a omic, e nang le genomics e bapisang, transcriptomics, tlhahlobo ea QTL ea baahi ba liphatsa tsa lefutso, e senotse phapang ea bohlokoa pakeng tsa R. wichuraiana le Rosa chinensis. Hape, phapang ea tlhahiso ea liphatsa tsa lefutso tse amanang le QTL e ne e ka amahanngoa le ho etsoa ha li-prickle patterning tsa kutu.
Tlhahlobo ea genomics e bapisoang pakeng tsa Thornless ea Basye le Rosa chinensis ho kenyeletsoa tlhahlobo ea synteny, sehlopha sa lelapa la liphatsa tsa lefutso, tlhahlobo ea katoloso le ho honyela, e senotse mefuta e mengata ea liphapang, tse amanang le litšobotsi tsa bohlokoa liroseng. Katoloso e ikhethang lelapeng la liphatsa tsa lefutso la NAC le FAR1/FRS e ne e ka amahanngoa le ho hanyetsa letheba le letšo.
Tlhahlobo ea li-genomics tse bapisoang pakeng tsa li-genome tsa BT le haploOB.
Zhong, M., et al. “Rose ntle le prickle: temohisiso ea liphatsa tsa lefutso e amanang le ho ikamahanya le mongobo”Tlhahlobo ea Saense ea Naha, 2021;, nwab092.
