● E hloka genome ea referense.
● Lambda DNA e sebelisetsoa ho beha leihlo katleho ea phetoho ea bisulfite.
● MspI tshilo ya dijo e boetse e behilwe leihlo.
● Ho sila ha li-enzyme tse habeli bakeng sa lisampole tsa semela.
● Sequencing ho Illumina NovaSeq.
●Mokhoa o mong o sebetsang hantle le o sebetsang hantle oa WGBS: ho nolofalletsa hore tlhahlobo e etsoe ka theko e tlase le ka litlhoko tse tlase tsa sampole.
●Sethala se Felletseng:fana ka ts'ebeletso e ntle ea sebaka se le seng ho tloha ts'ebetsong ea sampole, kaho ea laeborari, le tatellano ea tlhahlobo ea bioinformatics.
●Tsebo e Pharalletseng: ka merero ea tatellano ea RRBS e phethiloeng ka katleho mefuteng e fapaneng ea mefuta-futa, BMKGENE e tlisa phihlelo ea lilemo tse fetang leshome, sehlopha sa bahlahlobisisi ba nang le tsebo e phahameng, litaba tse feletseng, le tšehetso e babatsehang ea ka mor'a thekiso.
Laebrari | Leano la Tatelano | Tlhahiso ea data e khothalelitsoeng | Taolo ea boleng |
MspI e silehileng le laeborari ea phekolo ea Bisulfite | Illumina PE150 | 8 Gb | Q30 ≥ 85% Phetoho ea Bisulfite > 99% Katleho ea ho itšeha ea MspI> 95% |
Khokahano (ng/µL) | Kakaretso (µg) |
| |
Genomic DNA | ≥30 | ≥1 | Phokotso e fokolang kapa tshilafatso |
E kenyelletsa tlhahlobo e latelang:
● Taolo ea boleng ba tatellano e tala;
● Ho etsa 'mapa oa litšupiso tsa genome;
● Ho fumanoa ha 5mC methylated bases le boitsebiso ba motif;
● Tlhahlobo ea kabo ea methylation le papiso ea sampole;
● Tlhahlobo ea Libaka tse sa tšoaneng tsa Methylated (DMRs);
● Tlhaloso e sebetsang ea liphatsa tsa lefutso tse amanang le li-DMR.
Taolo ea boleng: ts'ebetso ea tšilo ea lijo (ka 'mapa oa genome)
Taolo ea boleng: phetolo ea bisulfite (ka ho ntšoa ha lintlha tsa methylation)
'Mapa oa Methylation: 5mC methylation genome-wide distribution
Papiso ea mohlala: Tlhahlobo ea Karolo e ka Sehloohong
Tlhahlobo ea Libaka tse fapaneng tsa Methylated (DMRs): 'mapa oa mocheso
Lekola tsoelo-pele ea lipatlisiso e tsamaisoang ke litšebeletso tsa tatellano ea liphatsa tsa lefutso tsa BMKGene ka pokello e hlophisitsoeng ea lingoliloeng.
Li, Z. et al. (2022) 'Ho tsosolosa botšepehi bo phahameng ka har'a lisele tse kang Leydig ka ts'ebetso ea CRISPR le lintlha tsa paracrine',PNAS Nexus, 1(4). doi: 10.1093/PNASNEXUS/PGAC179.
Tian, H. et al. (2023) 'Genome-wide DNA methylation analysis of body composition in Chinese monozygotic twins',European Journal of Clinical Investigation, 53(11), leq. e14055. doi: 10.1111/ECI.14055.
Wu, Y. et al. (2022) 'DNA methylation le karo-karolelano ea thekeng-to-hip: thuto ea epigenome-wide association mafahla a monozygotic a Chaena',Journal of Endocrinological Investigation, 45(12), leqepheng la 2365-2376. doi: 10.1007/S40618-022-01878-4.