Representation Bisulfite Sequencing (RRBS)
Litlhaloso tsa Tšebeletso
Sethala: Sethala sa NovaSeq, PE150
Mofuta oa laebrari: 200-400bp kenya laeborari ea DNA ea bisulfite e tšoaroang
Leano la tatellano: Paired-End 150 bp
Khothalletsa tlhahiso ea data: 10 Gb data e tala / sampole
Litlhoko tsa Mohlala
Palo ea DNA: ≥ 2ug
Khatiso ea DNA: ≥ 20ng/μl.
Bohloeki: OD260/280 = 1.8 ho 2.0 ntle le ho senyeha kapa ho silafala ha RNA
Tlhahlobo ea Bioinformatics
Phallo ea Mosebetsi oa Ts'ebeletso
Mohlala oa thomello
Kaho ea laebrari
Tatelano
Tlhahlobo ea lintlha
Litšebeletso tsa ka mor'a thekiso
a.Reference genome alignment statistics
Lipalopalo tsa ho bala 'mapa
Slipalo-palo tsa botebo ba tatellano le tšireletso ea sebaka sa C
Abokeletseng kgaso
Dbokhoni ba ho igestion ba sampole ka 'ngoe
b.Ho fumana sebaka sa methylation
Lethathamo la maemo a methylation sebakeng sa C
BS phetoho
Methylated cytosine
Kabo ea boemo ba methylation
c.'Mapa oa methylation
Gename-bophara
Genome element
d.Papiso ea maemo a methylation lipakeng tsa disampole
PAirwise Khokahano ea CG methylation
PCA-CG methylation
Cho khanya ha CG methylation
d.Tlhahlobo e fapaneng ea methylation
Slipalo-palo tsa DMR
Kabo ea bolelele ba DMR
Hja mmapa
DKabo ea MR sebakeng se reriloeng
