Tatellano ea Metagenomic -NGS
Metagenome ke pokello ea lintho tsohle tsa lefutso tsa sechaba se tsoakiloeng sa lintho tse phelang, joalo ka li-metagenome tsa tikoloho le tsa batho. E na le li-genome tsa likokoana-hloko tse ka lengoang le tse sa lengoang. Ho latellana ha metagenomic ka lithunya tsa lithunya ka NGS ho nolofalletsa ho ithuta libaka tsena tse rarahaneng tsa genomic tse kentsoeng lisampoleng tsa tikoloho ka ho fana ka ho fetang ho hlahloba taxonomic, ho fana ka temohisiso e qaqileng mabapi le mefuta-futa ea mefuta, matla a bongata, le meaho e rarahaneng ea baahi. Ntle le lithuto tsa taxonomic, shotgun metagenomics e boetse e fana ka pono e sebetsang ea genomics, e nolofalletsang ho hlahloba liphatsa tsa lefutso tse kentsoeng le mesebetsi ea tsona ea ho beha mabaka lits'ebetsong tsa tikoloho. Qetellong, ho thehoa ha marang-rang a kamano pakeng tsa likarolo tsa liphatsa tsa lefutso le lintlha tsa tikoloho ho thusa kutloisisong e akaretsang ea tšebelisano e rarahaneng pakeng tsa lichaba tsa likokoana-hloko le semelo sa tsona sa tikoloho. Qetellong, ho latellana ha metagenomic ho eme e le sesebelisoa sa bohlokoa bakeng sa ho manolla mathata a genomic a lichaba tse fapaneng tsa likokoana-hloko, ho bonesa likamano tse ngata pakeng tsa liphatsa tsa lefutso le tikoloho ka har'a tikoloho ena e rarahaneng.
Li-platform: Illumina NovaSeq le DNBSEQ-T7
Melemo ea Tšebeletso
●Mokhoa oa ho itšehla thajana le ho se leme bakeng sa ho hlahisa liprofaele tsa sechaba sa likokoana-hloko: Ho nolofalletsa ho latellana ha lintho tsa lefutso ho tsoa linthong tse sa lengoang.
●Qeto e Phahameng: Fumana mefuta e mengata haholo disampoleng tsa tikoloho.
●Tlhahlobo e Felletseng ea Bioinformatics:Ha ea ka ea shebana feela le mefuta-futa ea taxonomic empa hape le mefuta-futa ea mesebetsi ea sechaba.
●Phihlelo e pharaletseng:Ka rekoto ea katleho ea ho koala merero e mengata ea metagenomics libakeng tse fapaneng tsa lipatlisiso le ho sebetsana le lisampole tse fetang 200,000, sehlopha sa rona se tlisa boiphihlelo bo bongata morerong o mong le o mong.
Litlhaloso tsa Tšebeletso
| Sethala sa tatellano | Leano la Tatellano | Lintlha tse khothaletsoang | Taolo ea boleng |
| Illumina NovaSeq kapa DNBSEQ-T7 | PE150 | 6-20Gb | Q30≥85% |
Litlhoko tsa Tšebeletso
| Ho tsepamisa maikutlo (ng/µL) | Kakaretso ea chelete (ng) | Bophahamo ba modumo (µL) |
| ≥1 | ≥30 | ≥20 |
● Mobu/seretse: 2-3g
● Bongata ba mala - phoofolo: 0.5-2g
● Dikahare tsa mala - dikokonyana: 0.1-0.25g
● Bokaholimo ba semela (seretse se nontšitsoeng): 0.5-1g
● Moro o nonneng oa ho belisa: 0.2-0.5g
● Mantle (liphoofolo tse khōlō): 0.5-2g
● Mantle (toeba): lithollo tse 3-5
● Mokelikeli oa ho hlapa ka alveolar ea matšoafo: pampiri ea sefe
● Sesepa sa botšehali: lisepa tse 5-6
● Sesepa sa letlalo/setho sa botona/mathe/dinama tse bonolo tsa molomo/sesepa sa pharyngeal/sesepa sa rectal: disepa tse 2-3
● Likokoana-hloko tse ka holimo: li-swab tse 5-6
● 'Metsi/moea/biofilm: pampiri ea sefe
● Li-endophyte: 2-3g
● Letlapa la meno: 0.5-1g
Phallo ea Mosebetsi oa Tšebeletso
Phano ea mohlala
Kaho ea laeborari
Tatellano
Tlhahlobo ea lintlha
Litšebeletso tsa kamora thekiso
E kenyelletsa tlhahlobo e latelang:
● Taolo ea boleng ba data e latellanang
● Kopano ea metagenome le ho bolela esale pele liphatsa tsa lefutso
● Tlhaloso ea liphatsa tsa lefutso
● Tlhahlobo ea mefuta-futa ea taxonomic alpha
● Tlhahlobo ea ts'ebetso ea sechaba: ts'ebetso ea baeloji, metabolism, ho hanyetsa lithibela-mafu
● Tlhahlobo mabapi le mefuta-futa ea mesebetsi le ea taxonomic:
Tlhahlobo ea mefuta-futa ea beta
Tlhahlobo ea lihlopha tse fapaneng
Tlhahlobo ea kamano: lipakeng tsa mabaka a tikoloho le sebopeho le mefuta-futa ea OUT
Tlhahlobo ea ts'ebetso: Khanyetso ea lithibela-mafu ea CARD
Tlhahlobo e fapaneng ea litsela tsa metabolism tsa KEGG: 'mapa oa mocheso oa litsela tsa bohlokoa
Mefuta-futa ea kabo ea taxonomic ea Alpha: Index ea ACE
Mefuta-futa ea beta ea kabo ea taxonomic: PCoA
Hlahloba tsoelo-pele e tsamaisoang ke lits'ebeletso tsa tatellano ea metagenome tsa BMKGene ka Illumina ka pokello ea lingoliloeng tse hlophisitsoeng.
Hai, Q. et al. (2023) 'Tlhahlobo ea metagenomic le metabolomic ea liphetoho tse ka hare ho mala a rainbow trout (Oncorhynchus mykiss) e tšoaelitsoeng ke kokoana-hloko ea hematopoietic necrosis e tšoaetsanoang mochesong o fapaneng oa metsi a setso',Meeli ho Microbiology, 14, leq. 1275649. doi: 10.3389/FMICB.2023.1275649.
Mao, C. et al. (2023) 'Metse ea likokoana-hloko, liphatsa tsa lefutso tsa khanyetso, le likotsi tsa khanyetso matšeng a litoropong a linaha tse fapaneng tsa trophic: Lihokelo tsa ka hare le litšusumetso tsa kantle',Koranta ea Tsoelo-pele ea Lintho tse Kotsi,9, leq. 100233. doi: 10.1016/J.HAZADV.2023.100233.
Su, M. et al. (2022) 'Tlhahlobo ea Metagenomic e Senotse Liphapang tsa Sebopeho le Ts'ebetso Pakeng tsa Likokoana-hloko tse Amanang le Metsi le tse Amanang le Tiile tsa Nku ea Rumen',Meeli ho Microbiology, 13, leq. 851567. doi: 10.3389/FMICB.2022.851567.
Yin, J. et al. (2023) 'Microbiota e nonneng e nkiloeng ke kolobe ea Ningxiang e nchafatsa metabolism ea carnitine ho khothaletsa ho kenngoa ha asiti ea mafura a mesifa likolobeng tse se nang mafura a DLY',Boiqapelo, 4(5), leq. 100486. doi: 10.1016/J.XINN.2023.100486.
Zhao, X. et al. (2023) 'Temohisiso ea metagenomic mabapi le likotsi tse ka bang teng tsa polasetiki e emelang lintho tse phelang/tse sa senyeheng le lithōle tse seng tsa polasetiki libakeng tse ka holimo le tse ka tlase tsa Haihe Estuary, Chaena',Saense ea Tikoloho e Felletseng, 887, leq. 164026. doi: 10.1016/J.SCITOTENV.2023.164026.
