Lilaebrari tse entsoeng esale pele tsa Illumina
Likaroloana
●Li-platform:Illumina NovaSeq 6000 le NovaSeq X Plus
●Mekhoa ea ho latellana ha lintho:PE150 le PE250
●Taolo ea boleng ba lilaebrari pele ho hlophisoa tatellano
●Ho hlophisa QC ea data le ho e isa ka tatellano:ho tlisoa ha tlaleho ea QC le data e tala ka mokhoa oa fastq kamora ho arola le ho sefa lipalo tsa Q30
Melemo ea Tšebeletso
●Ho feto-fetoha ha lits'ebeletso tsa ho latellana ha lintho:moreki a ka khetha ho latellana ka tsela, sele ea phallo, kapa ka bongata ba data bo hlokahalang (ho latellana ha tsela e sa fellang).
●Phihlelo e pharaletseng sethaleng sa tatellano ea Illumina:ka merero e likete e koetsoeng e nang le mefuta e fapaneng.
●Ho tlisoa ha tlaleho ea QC ea tatellano:ka litekanyo tsa boleng, ho nepahala ha data le ts'ebetso ka kakaretso ea projeke ea tatellano.
●Mokhoa oa ho hlophisa lintho tse holileng:ka nako e khutšoanyane ea ho reteleha.
●Taolo e Matla ea Boleng: re kenya tshebetsong ditlhoko tse tiileng tsa QC ho netefatsa hore re fumana diphetho tsa boleng bo hodimo ka dinako tsohle.
Mehlala ea Li-platform
| Sethala | Sele ea Phallo | Mokhoa oa ho latellana | Yuniti | Sephetho se hakantsoeng |
| NovaSeq X | 10B (litsela tse 8) | PE150 | Single Lane Tsela e sa fellang | 375Gb / Tsela |
| 25B (litsela tse 8) | PE150 | Single Lane Tsela e sa fellang | 1000 Gb/Mola | |
| NovaSeq 6000 | Sele ea Phallo ea SP (litsela tse 2) | PE250 | Sele ea Phallo Single Lane Tsela e sa fellang | Lipalo tse 325-400 M / Tsela |
| Sele ea phallo ea S4 (methapo e 4) | PE150 | Sele ea Phallo Single Lane Tsela e sa fellang | ~800 Gb / Tsela |
Litlhoko tsa Sampole
| Palo ea Lintlha (X) | Ho tsepamisa maikutlo (qPCR/nM) | Molumo | |
| Tatellano ea Litsela tse sa fellang
| X ≤ 10 Gb | ≥ 1 nM | ≥ 25 μl |
| 10 Gb < X ≤ 50 Gb | ≥ 2 nM | ≥ 25 μl | |
| 50 Gb < X ≤ 100 Gb | ≥ 3 nM | ≥ 25 μl | |
| X > 100 Gb | ≥ 4 nM | ≥ 25 μl | |
| Tatellano ea Litsela | Ka Tsela | ≥ 1.5 nM / Letangoana la ho sesa la laeborari | ≥ 25 μl / Letangoana la ho sesa la laeborari |
Ntle le ho tsepamisa maikutlo le bongata bohle, mokhoa o loketseng oa tlhoro le oona oa hlokahala.
Hlokomela: Ho latellana ha litsela tsa lilaebrari tsa mefuta e fokolang ho hloka hore PhiX e kene ho netefatsa hore ho na le sebaka se matla sa ho fihlella.
Re khothaletsa ho romela lilaebrari tse kopantsoeng esale pele e le mehlala. Haeba o hloka hore BMKGENE e etse pokello ea lilaebrari, ka kopo sheba
litlhoko tsa laeborari bakeng sa tatellano ea litsela tse sa fellang.
Boholo ba Laeborari ('Mapa oa tlhoro)
Tlhōrō e kholo e lokela ho ba ka hare ho 300-450 bp.
Lilaeborari li lokela ho ba le tlhōrō e le 'ngoe e kholo, ho se silafatsoe ke adaptara le ho se be le li-primer dimers.
Ka kopo ikopanye le rona haeba disampole tsa hau li sa fihlelle litlhoko tsa thepa ea ho qala.
Mokhoa oa Tšebeletso
Taolo ea boleng ba laeborari
Tatellano
Taolo ea boleng ba data
Phepelo ea projeke
Tlaleho ea QC ea Laeborari
Tlaleho ea boleng ba laeborari e fanoa pele ho hlophisoa tatellano, ho lekolwa bongata ba laeborari le ho aroloa ha likarolo.
Tlaleho ea QC ea tatellano
Tafole ea 1. Lipalopalo mabapi le lintlha tsa tatellano.
| ID ea mohlala | BMKID | Libuka tse tala | Lintlha tse tala (bp) | Lipalo tse hloekileng (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Setšoantšo sa 1. Kabo ea boleng hammoho le lipalo sampole ka 'ngoe
Setšoantšo sa 2. Kabo ea motheo ea litaba
Setšoantšo sa 3. Kabo ea litaba tse baliloeng ka tatellano ea lintlha
