Lihlahisoa

Prokaryotic RNA tatelano

Prokaryotic RNA sequencing Featured Image

Prokaryotic RNA sequencing e sebelisa tatellano ea moloko o latelang (NGS) ho senola boteng le bongata ba RNA ka nako e itseng, ka ho hlahloba phetoho ea cellular transcriptome.K'hamphani ea rona ea prokaryotic RNA sequencing, e ikemiselitse haholo ho li-prokaryotes tse nang le liphatsa tsa lefutso, ho u fa profiling ea transcriptome, tlhahlobo ea sebopeho sa liphatsa tsa lefutso, joalo-joalo. E sebelisitsoe haholo lipatlisisong tsa mahlale a mantlha, lipatlisiso tsa lithethefatsi le nts'etsopele, le tse ling.

Sethala: Illumina NovaSeq Platform

Iteanye le rona

Lintlha tsa Tšebeletso

Litlhaloso tsa Tšebeletso

Sethala: sethala sa Illumina NovaSeq, PE150

Mofuta oa laebrari: 350bp kenya laeborari ea cDNA (e ipapisitse le kabo ea tlhoro)

Leano la tatellano: Paired-End 150 bp

Khothaletsa palo ea data: 2 Gb data/sample

Bioinformatics

(1) Ho latela taolo ea boleng ba data: Kabo ea Nucleotide, Kabo ea boleng ba Base, tlhahlobo ea karolelano ea rRNA;

(2) Tlhahlobo ea tatellano ea tatellano: Lipalo-palo tsa sephetho sa ho lumellana, Saturation ea tatellano, Ho koaheloa ha tatellano;

(3) Tlhaloso ea liphatlalatso tsa genome (NR, Swiss-Prot, Pfam, COG, GO, KEGG);

(4) Tlhaloso ea maikutlo: Kabo ea maikutlo (Ka mehlala e 'meli kapa ho feta), Tlhahlobo ea polelo pakeng tsa mehlala (tlhahlobo ea Venn, tlhahlobo ea Correlation, tlhahlobo ea PCA);

(5) Tlhahlobo ea polelo e fapaneng (Ka mehlala e 'meli kapa ho feta);

(6) Tlhahlobo ea liphatsa tsa lefutso: Tlhahlobo ea Venn, tlhahlobo ea Cluster, Tlhahlobo ea tlhaloso ea mosebetsi (COG, GO, KEGG), Tlhahlobo ea ntlafatso ea mosebetsi (GO, KEGG), graph ea ntlafatso ea mosebetsi, tlhahlobo ea Ipath;

(8) Tlhahlobo ea Sebopeho sa Transcript: Tlhahlobo ea Operon, Transcription ea ho qala le ho qetela ho bolela esale pele libaka, tlhaloso le tlhahlobo ea UTR, Polelo ea tatellano ea Promoter, tlhahlobo ea SNP / InDel (SNP / InDel annotation, SNP / InDel kabo ea libaka, lipalo-palo tsa SNP).