BMKGENE e motlotlo ho phatlalatsa boteng ba PacBio, Revio, e re thusang ho fana ka litšebeletso tsa tatellano ea boleng bo holimo le tsa boleng bo holimo ho bafuputsi.
*Hobaneng o Khetha PacBio Revio?
* Likopo tsa PacBio Revio
•Tatelano ya genome
1.Ho tloha Novokopano ea genome- Revio e kenya letsoho tatellanong ea boleng bo holimo le ho bokelleng ha liphatsa tsa lefutso tse kholo ka ho fetesisa le tse rarahaneng, e fana ka leseli la bohlokoa mabapi le phapang ea liphatsa tsa lefutso, sebopeho sa liphatsa tsa lefutso le tšebetso.
2.T2T genome- Revio, e kopantsoeng le mekhoa e kang Hi-C, e ka ntlafatsa boleng ba liphatsa tsa lefutso tse tloaelehileng le ho thusa mefuta e mengata ea mefuta e mengata ho fumana genome ea litšupiso tsa boemo ba T2T, e leng ho nolofalletsang bafuputsi ho utloisisa hamolemo mefuta-futa ea liphatsa tsa lefutso ka hare le pakeng tsa baahi.
3.Tatelano ea liphatsa tsa lefutso kaofela- Revio e ka fana ka lintlha tse felletseng mabapi le sebopeho le tšebetso ea liphatsa tsa lefutso kaofela, ho kenyeletsoa SNP, Indel, SV, CNV, joalo-joalo, haholo-holo bakeng sa liphatsa tsa lefutso tse kholo.
4.Pan-genome tatelano- Revio e loketse ho hlophisa le ho kopanya mefuta e mengata ea liphatsa tsa lefutso ka nako e le 'ngoe ho aha pan-genome, e fana ka lintlha tsa bohlokoa mabapi le phapano ea genomic le ho iphetola ha lintho.
•Tatelano ya transcriptome
1.Tatelano ya sengoloa sa bolelele bo felletseng- Ka bolelele ba eona ba ho bala nako e telele, Revio e thusa tatellano ea sengoloa sa boleng bo felletseng, e fana ka leseli le felletseng mabapi le mokhoa o mong oa ho arola, ho kopanya liphatsa tsa lefutso le polelo ea isoform.
2.Tlhaloso ea bolelele bo felletseng ba lisampole tse kholo- Revio e ka fana ka palo e nepahetseng haholo ea maemo a polelo ea liphatsa tsa lefutso ho lisampole tse kholo, e nolofalletsang lintlha tsa bohlokoa mabapi le taolo ea liphatsa tsa lefutso le phapang ea lisele.
3.Tatelano ea transcriptome ea sele e le 'ngoe- Revio e ka fana ka lintlha tse phahameng tsa li-transcriptomics tsa sele e le 'ngoe, e leng ho nolofalletsang bafuputsi ho ithuta heterogeneity ea lisele le ho tseba mefuta e sa tloaelehang ea lisele.
•Tatelano ya microbial
1.16S tatellano ea bolelele bo felletseng- Revio e thusa 16S rRNA tatelano ea boleng bo holimo, e fana ka lintlha tse qaqileng mabapi le sebopeho le tšebetso ea sechaba sa likokoana-hloko.
2.Tatelano ea amplicon ea nako e telele- Revio e loketse hantle tatellano ea amplicon e baloang nako e telele, e nolofalletsang bafuputsi ho ithuta ka mefuta-futa ea likokoana-hloko tikolohong e rarahaneng.