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Kugereranya Genomika
Kugereranya genomics mubyukuri bisobanura kugereranya genome yuzuye hamwe nuburyo bwubwoko butandukanye.Iyi disipuline igamije kwerekana ubwihindurize bwibinyabuzima, imikorere ya gene, uburyo bwo kugenzura imiterere ya gen mu rwego rwo kumenya imiterere n'ibintu byakurikiranye cyangwa bitandukanya amoko atandukanye.Ubushakashatsi bugereranya genomics burimo gusesengura mumuryango wa gene, iterambere ryubwihindurize, kwigana genome yose, igitutu cyo guhitamo, nibindi.
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Urutonde rwa ADN / RNA -PacBio Ikurikirana
Urubuga rwa PacBio rukurikirana ni urubuga rwasomwe igihe kirekire, ruzwi kandi nka bumwe mu buhanga bwa gatatu (TGS).Tekinoroji yibanze, molekile imwe-nyayo-nyayo (SMRT), iha imbaraga ibisekuruza byasomwe hamwe na kilo-shingiro muburebure.Hishimikijwe "Gukurikirana-by-Synthesis", gukemura nucleotide imwe bigerwaho na Zero-mode waveguide (ZMW), aho ijwi rito gusa hepfo (site ya synthesis ya molekile), rimurikirwa.Mubyongeyeho, urutonde rwa SMRT rwirinda ahanini kubogama kubogamye muri sisitemu ya NGS, kuberako intambwe nyinshi zo kongera PCR zidasabwa mubikorwa byo kubaka isomero.
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Isesengura ryinshi rya Segregant
Isesengura ryinshi (BSA) nubuhanga bukoreshwa mukumenya byihuse ibimenyetso bya genotype.Ibikorwa nyamukuru bya BSA bikubiyemo gutoranya amatsinda abiri yabantu bafite fenotipike irwanya cyane, guhuza ADN yabantu bose kugirango babeho ibice bibiri bya ADN, bikerekana itandukaniro riri hagati yibidendezi bibiri.Ubu buhanga bwakoreshejwe cyane mukumenya ibimenyetso bya genetike bifitanye isano na gen zigenewe ibimera / inyamaswa.
